Date published: 2025-9-6
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p-Histone H3 Antibody (C-2) - Western Blotting - Image 155750
未经处理 (A, D), 钙霉素 A 处理 (B, E) 和钙霉素 A 及λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 Jurkat 全细胞裂解液中组蛋白 H3 磷酸化的 Western 印迹分析. 测试的抗体包括 p-组蛋白 H3 抗体 (C-2): sc-374669 (A, B, C) 和组蛋白 H3 (FL-136): sc-10809 (D, E, F).
p-Histone H3 Antibody (C-2) - Western Blotting - Image 155750
未经处理 (A, D), 钙霉素 A 处理 (B, E) 和钙霉素 A 及λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 Jurkat 全细胞裂解液中组蛋白 H3 磷酸化的 Western 印迹分析. 测试的抗体包括 p-组蛋白 H3 抗体 (C-2): sc-374669 (A, B, C) 和组蛋白 H3 (FL-136): sc-10809 (D, E, F).
未经处理 (A, D), 钙霉素 A 处理 (B, E) 和钙霉素 A 及λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 Jurkat 全细胞裂解液中组蛋白 H3 磷酸化的 Western 印迹分析. 测试的抗体包括 p-组蛋白 H3 抗体 (C-2): sc-374669 (A, B, C) 和组蛋白 H3 (FL-136): sc-10809 (D, E, F).

p-Histone H3 抗体 (C-2): sc-374669

4.3(10)
寫評論提問

说明书
  • p-Histone H3抗体(C-2)是小鼠单克隆IgG2b κ, 在30篇文献中引用，规格为200 µg/ml
  • p-Histone H3 抗体 (C-2) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的Ser 28 phosphorylated Histone H3; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗p-Histone H3抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-374669 X, 200 µg/0.1 ml)
  • 关于如何获取p-Histone H3 (C-2): sc-374669的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是p-Histone H3 Antibody (C-2) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与p-Histone H3 Antibody (C-2) 打包提供（请参阅下面的订购信息）。

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说明书与实验方案
研究信息

関連項目

附加Histone抗体，包括Histone cluster 1 H1, Histone H2A, Histone H2B, Histone H3, Histone H4和Histone cluster 1 H3A

p-Histone H3抗体（C-2）是一种IgG2b κ小鼠单克隆p-Histone H3抗体（也称为磷酸化组蛋白H3抗体），它通过WB, IP, IF和ELISA检测小鼠、大鼠和人类来源的Ser 28磷酸化组蛋白H3。p-Histone H3抗体（C-2）可以非偶联形式的抗p-Histone H3抗体，也可以是多种偶联形式的抗p-Histone H3抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。在真核生物中，DNA缠绕在组蛋白八聚体上，形成染色质结构的基本单位。八聚体由组蛋白H2A、H2B、H3和H4组成，它与大约200个碱基对的DNA结合形成核小体。DNA与组蛋白的结合导致染色质密集包装，这限制了参与基因转录的蛋白质与DNA的结合。核小体的核心蛋白组蛋白H3在前期结束时被磷酸化。磷酸化的两个主要位点是丝裂原特异性位点Ser 10和Ser 28，两者在DNA结合形式的组蛋白H3和核小体组蛋白H3中都广泛磷酸化。组蛋白H3的核小体结构在体外促进了N末端磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Histone H3 抗体 (C-2) 参考文献:

  1. 中心异染色质的减数分裂事件：组蛋白 H3 磷酸化, 拓扑异构酶 II alpha 定位和染色体凝聚。  |  Cobb, J., et al. 1999. Chromosoma. 108: 412-25. PMID: 10654080
  2. ERKs 和 p38 激酶介导紫外线 B 诱导的组蛋白 H3 丝氨酸 10 磷酸化。  |  Zhong, SP., et al. 2000. J Biol Chem. 275: 20980-4. PMID: 10806218
  3. 肝细胞癌中Aurora-B激酶和磷酸化组蛋白H3的表达。  |  Sistayanarain, A., et al. 2006. Anticancer Res. 26: 3585-93. PMID: 17094487
  4. 有丝分裂中CHO细胞中Thr3-磷酸化组蛋白H3的动态分布。  |  Zhou, H., et al. 2006. Folia Biol (Praha). 52: 156-60. PMID: 17116287
  5. 良性及非典型颗粒细胞肿瘤中的磷酸化组蛋白 H3、Ki-67、p21、脂肪酸合成酶和裂解的半胱氨酸蛋白酶-3 表达。  |  Kapur, P., et al. 2007. Arch Pathol Lab Med. 131: 57-64. PMID: 17227124
  6. 通过组合修饰模式调节 14-3-3 与磷酸化组蛋白 H3 的相互作用。  |  Winter, S., et al. 2008. Cell Cycle. 7: 1336-42. PMID: 18418070
  7. 体外核小体结构中有丝分裂特异性组蛋白 H3 磷酸化。  |  Shibata, K., et al. 1990. Eur J Biochem. 192: 87-93. PMID: 2401299
  8. H1 和核心组蛋白亚型：不同的基因表达和不同的一级结构。  |  Doenecke, D., et al. 1988. Adv Enzyme Regul. 27: 107-20. PMID: 3250226
  9. 使用Ki-67增殖指数、磷酸化组蛋白H3（PHH3）和BCL-2，改进细针抽吸术以预测胰腺神经内分泌肿瘤的肿瘤生物学侵袭性。  |  Zhao, CL., et al. 2023. Ann Diagn Pathol. 65: 152149. PMID: 37119647
  10. 组蛋白 H1。  |  Wolffe, AP. 1997. Int J Biochem Cell Biol. 29: 1463-6. PMID: 9570139

订购信息

产品名称产品编号规格价格数量收藏夹

p-Histone H3 抗体 (C-2)

sc-374669
200 µg/ml
RMB2377.00

p-Histone H3 (C-2): m-IgG Fc BP-HRP 套装

sc-525987
200 µg Ab; 10 µg BP
RMB2662.00

p-Histone H3 (C-2): m-IgG2b BP-HRP 套装

sc-549654
200 µg Ab; 10 µg BP
RMB2662.00

p-Histone H3 抗体 (C-2) X

sc-374669 X
200 µg/0.1 ml
RMB2377.00

p-Histone H3 抗体 (C-2) AC

sc-374669 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Histone H3 抗体 (C-2) HRP

sc-374669 HRP
200 µg/ml
RMB2377.00

p-Histone H3 抗体 (C-2) FITC

sc-374669 FITC
200 µg/ml
RMB2482.00

p-Histone H3 抗体 (C-2) PE

sc-374669 PE
200 µg/ml
RMB2580.00

p-Histone H3 抗体 (C-2) Alexa Fluor® 488

sc-374669 AF488
200 µg/ml
RMB2685.00

p-Histone H3 抗体 (C-2) Alexa Fluor® 546

sc-374669 AF546
200 µg/ml
RMB2685.00

p-Histone H3 抗体 (C-2) Alexa Fluor® 594

sc-374669 AF594
200 µg/ml
RMB2685.00

p-Histone H3 抗体 (C-2) Alexa Fluor® 647

sc-374669 AF647
200 µg/ml
RMB2685.00

p-Histone H3 抗体 (C-2) Alexa Fluor® 680

sc-374669 AF680
200 µg/ml
RMB2685.00

p-Histone H3 抗体 (C-2) Alexa Fluor® 790

sc-374669 AF790
200 µg/ml
RMB2685.00

p-Histone H3 (C-2) 中和勝肽

sc-374669 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-374669 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good product!This antibody works well on western of rat tissue.
Date published: 2019-04-22
Rated 4 out of 5 by from worth to buy itAfter I bought it and used it for a pretty long time, and I think this antibody is worth to be used for my expertiments. The signals was strong, I could read it very easy, the most important thing the backgroud of my membranes are much more clean than other antibody.
Date published: 2017-12-22
Rated 5 out of 5 by from Great antibody for IFWorks well for IF in hunam HT1080 cells at 1:400 dilution.
Date published: 2017-09-27
Rated 3 out of 5 by from Not compatible with our second antibodyI was planning to use this antibody for western blotting control and just realized that our secondary antibody was anti-mouse M2 conjugated to Cy3. So basically these antibodies were not compatible and I didn't see any histone signals. We are now looking for another way to see histone signals as a loading control.
Date published: 2017-09-18
Rated 5 out of 5 by from So nice band.I am satisfied with this antibody because I got the band that I want.
Date published: 2017-08-31
Rated 5 out of 5 by from Great antibody!works really good for IF on mouse brain tissue. Dilution is 1:200.
Date published: 2017-02-22
Rated 4 out of 5 by from An useful antibody!P-histone H3 (SER28) antibody is suitable for western blot analysis in AGS cells，we obtained results
Date published: 2017-02-10
Rated 4 out of 5 by from Worked fineIt worked fine for western blotting. I did see some nonspecific signal but not near the main signal
Date published: 2016-12-20
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p-Histone H3 抗体 (C-2) is rated 4.3 out of 5 by 10.
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