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p-ERK 1/2 Antibody (12D4) - Western Blotting - Image 62925
p-ERK 1/2 抗体 (12D4): sc-81492. 对未受刺激 (A), EGF 刺激 (B) 和过钒酸盐处理 (C) 的 SKOV3 全细胞裂解液中 ERK 1/2 磷酸化的 Western 印迹分析.
p-ERK 1/2 Antibody (12D4) - Western Blotting - Image 62925
p-ERK 1/2 抗体 (12D4): sc-81492. 对未受刺激 (A), EGF 刺激 (B) 和过钒酸盐处理 (C) 的 SKOV3 全细胞裂解液中 ERK 1/2 磷酸化的 Western 印迹分析.
p-ERK 1/2 Antibody (12D4) - Western Blotting - Image 71968
未经处理 (A, D), 经 PMA 处理 (B, E) 以及经 PMA 和 lambda 蛋白磷酸酶处理 (C, F) 的 Jurkat 全细胞裂解液中 ERK 1/2 磷酸化的 Western 印迹分析. 测试的抗体包括 p-ERK 1/2 抗体 (12D4): sc-81492 (A, B, C) 和 ERK 2 (K-23): sc-153 (D, E, F).
p-ERK 1/2 抗体 (12D4): sc-81492. 对未受刺激 (A), EGF 刺激 (B) 和过钒酸盐处理 (C) 的 SKOV3 全细胞裂解液中 ERK 1/2 磷酸化的 Western 印迹分析.

p-ERK 1/2 抗体 (12D4): sc-81492

4.9(11)
寫評論提問

说明书
  • p-ERK 1/2 抗体 12D4 是小鼠单克隆 IgG1, 在254篇文献中引用，提供 50 µg/0.5 ml
  • 针对源自human的ERK1的T-E-Y结构域周围氨基酸残基的合成磷酸化肽段
  • 推荐用于 mouse, rat, human 和 canine 来源的Thr 202 and Tyr 204 dually phosphorylated ERK 1 and ERK 2 WB, IP 和 IF检测; 不会与 non-phosphorylated ERK 1/2产生交联反应; 需要在Thr和Tyr位点进行磷酸化
  • 看 p-ERK (E-4): sc-7383，可查看有标记的 p-ERK 1/2 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取p-ERK 1/2 (12D4): sc-81492的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成p-ERK 1/2 抗体 (12D4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 ERK 抗体包括 ERK 1, ERK 2, ERK 3 和 ERK 5

p-ERK 1/2 抗体（12D4）是一种小鼠单克隆 IgG1 抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF ) 应用。p-ERK 1/2 单克隆抗体 (12D4) 采用非结合形式，专门用于靶向 Thr 202 和 Tyr 204 处 ERK 1 和 ERK 2 的双重磷酸化形式。ERK蛋白是细胞分裂素激活蛋白激酶（MAPK）信号传导通路的一部分，在调节细胞对生长因子、激素和神经递质的反应中起着至关重要的作用。p-ERK 1/2结构的重要性在于其双重磷酸化机制，这是激活的关键。ERK1中Thr 202和Tyr 204以及ERK2中Thr 185和Tyr 187的磷酸化会导致显著的结构变化，包括活性位点的关闭以及与ATP相互作用的催化残基的正确排列。这些结构变化对于ERK蛋白的酶活性至关重要，使ERK蛋白能够对丝氨酸和苏氨酸残基上的下游靶标进行磷酸化，从而传播信号级联。此外，ERK蛋白的调节还涉及上游激活因子，如MAP激酶激酶（MEK）、MEK激酶和Raf-1，这凸显了调控细胞功能的信号通路网络的复杂性。ERK家族还包括另外三个成员： ERK 3、ERK 5和ERK 6，这进一步强调了信号通路在各种生物过程中的复杂性和重要性。抗-p-ERK 1/2抗体（12D4）是研究ERK 1/2在Thr 202和Tyr 204处的双重磷酸化的重要工具，这种关键的修饰可激活并促进MAPK通路中的下游信号传导。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-ERK 1/2 抗体 (12D4) 参考文献:

  1. ERK1和ERK2是两种微管相关蛋白2激酶，它们在原位介导酪氨酸羟化酶丝氨酸-31的磷酸化。  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
  2. 纯化磷酸化并激活 Erk-1 基因产物的鼠蛋白酪氨酸/苏氨酸激酶：与裂殖酵母 byr1 基因产物的关系。  |  Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
  3. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  4. 细胞外信号调节激酶 1（一种胰岛素刺激的微管相关蛋白 2 激酶）的纯化和特性。  |  Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
  5. 确定 pp42/丝裂原活化蛋白激酶（MAP 激酶）的调节磷酸化位点。  |  Payne, DM., et al. 1991. EMBO J. 10: 885-92. PMID: 1849075
  6. ERK：丝氨酸/苏氨酸蛋白激酶家族，在胰岛素和 NGF 的作用下被激活并酪氨酸磷酸化。  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
  7. P-AKT2/SPK1 (P-SPK1)和P-MEK/P-ERK细胞信号传导通路参与LPS诱导的巨噬细胞迁移。  |  Lei, Y., et al. 2019. Am J Transl Res. 11: 2725-2741. PMID: 31217849
  8. IL-6/p-BTK/p-ERK信号传导介导磷酸钙引起的瘙痒。  |  Keshari, S., et al. 2019. FASEB J. 33: 12036-12046. PMID: 31365830
  9. 一种新的人类蛋白激酶信号转导途径的组成部分。  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
  10. 参与 C2C12 肌母细胞分化的有丝分裂原激活蛋白激酶 ERK6  |  Lechner, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 4355-9. PMID: 8633070

订购信息

产品名称产品编号规格价格数量收藏夹

p-ERK 1/2 抗体 (12D4)

sc-81492
50 µg/0.5 ml
RMB2377.00

How many amino acids in length is the antigen phosphopeptide of this antibody?

Asked by: tarotaro27
Thank you for your inquiry. This antibody is raised against synthetic phosphopeptide corresponding to amino acid residues surrounding the T-E-Y motif of ERK1 of human origin. Unfortunately we do not known the exactly amino acids sequence of the antigen. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Sen Li
Date published: 2021-11-23

What is the complete amino acid sequence of the antigen phosphopeptide used as a source of this antibody?

Asked by: tarotaro27
Thank you for your inquiry. this antobody is raised against a short amino acid sequence containing dually phosphorylated Thr 202 and Tyr 204 of ERK 1 of rat origin.
Answered by: Sen Li
Date published: 2021-11-16

Does this antibody (sc-81492) recognize both dually phosphorylated ERK 1 (thr202 and tyr204) and ERK 2 (thr185 and tyr187)? I have seen only one band in the datasheet.

Asked by: Biologist
Thank you for your question. Yes, this antibody, p-ERK 1/2 (12D4): sc-81492 is recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 and ERK 2 of mouse, rat, human and canine origin by WB, IP and IF. It is non cross-reactive with non-phosphorylated ERK 1/2, and requires phosphorylation at both the Thr and Tyr sites for reactivity. ERK1 and ERK 2 are very similar in size, and we do not typically observe a doublet or more than one band when using this antibody in Western blotting.
Answered by: Technical Support
Date published: 2022-03-29

Is p-ERK 1/2 Antibody (12D4): sc-81492 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using p-ERK (E-4), which is available conjugated to AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. (https://www.scbt.com/scbt/product/p-erk-antibody-e-4)
Answered by: Technical Support 2
Date published: 2018-04-22
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Rated 4 out of 5 by from Great antibodyGreat bands and specific signal. But I had to load 80μg protein to detect it. Maybe it was due to my cell line (HaCat cells)
Date published: 2021-03-22
Rated 5 out of 5 by from Great antibodyIt specifically detects the protein of interest. Working dilution:1:200.
Date published: 2019-11-11
Rated 5 out of 5 by from great antibodyI like to work with santa cruz antibodies. They work great!
Date published: 2019-11-05
Rated 5 out of 5 by from Excellent antibodyExcellent antibody for detection of phosphorylated Erk. No background even with higher dilution
Date published: 2019-03-19
Rated 5 out of 5 by from OutstandingVery clear phospho- specific results, low background
Date published: 2017-01-07
Rated 5 out of 5 by from Dynamite in your WBThis antibody offers tried and true results. It will present clear and specific bands of p-ERK. The data is convincing, but over 30 citations are even more so.
Date published: 2016-12-29
Rated 5 out of 5 by from Good Western blot data in analyzing ERK 1/2Good Western blot data in analyzing ERK 1/2 phosphorylation in untreated, PMA treated, and PMA and lambda protein phosphatase treated Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Published with this antibody for WesternPublished with this antibody for Western blot in C4-2B cells. -SCBT Publication Review
Date published: 2015-03-29
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p-ERK 1/2 抗体 (12D4) is rated 4.9 out of 5 by 11.
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