Date published: 2026-10-24

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p-EGFR 抗体 (9H2): sc-57545

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说明书
  • p-EGFR 抗体 9H2 是小鼠单克隆 IgG1, 在12篇文献中引用,提供 50 µg/0.5 ml
  • 针对human来源的EFGR磷酸化肽段
  • 推荐用于 mouse, rat, human 和 canine 来源的Tyr 1173 phosphorylated EGFR WB, IP 和 IF检测; 与non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins不交叉反应
  • 看 EGFR (A-10): sc-373746,可查看有标记的 p-EGFR 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是p-EGFR Antibody (9H2) for WB applications. 的首选辅助检测试剂,这些试剂现与p-EGFR Antibody (9H2) 打包提供(请参阅下面的订购信息)。
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p-EGFR 抗体 (9H2) 是一种小鼠单克隆 IgG1 抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP) 和免疫荧光 (IF) 等应用检测小鼠、大鼠、人和犬样本中 Tyr 1173 磷酸化的表皮生长因子受体。表皮生长因子受体(EGFR)在细胞信号传导中起着至关重要的作用,它介导细胞对生长因子的反应,而生长因子对细胞增殖、分化和存活等过程至关重要。表皮生长因子受体主要位于细胞表面,与 EGF 或 TGFα 等配体结合后会被激活,从而引发一连串对细胞正常功能和发育至关重要的细胞内信号通路。特定酪氨酸残基(尤其是 Tyr 1173)的磷酸化对表皮生长因子受体的激活和后续信号传导至关重要,因为磷酸化有利于下游信号蛋白的招募,包括 GRB2 的磷酸酪氨酸结合域。这种相互作用是激活 Ras 信号通路的关键,而 Ras 信号通路是细胞生长和分裂不可或缺的。表皮生长因子受体(EGFR)信号传导失调往往与各种癌症有关,因此 p-EGFR (9H2) 抗体是研究癌症生物学和治疗干预的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-EGFR 抗体 (9H2) 参考文献:

  1. 磷酸化EGFR的核表达与食管鳞状细胞癌患者的预后不良有关。  |  Hoshino, M., et al. 2007. Pathobiology. 74: 15-21. PMID: 17496429
  2. 在吉非替尼敏感的非小细胞肺癌细胞系中,EGFR有效磷酸化以及磷酸化EGFR通过早期/晚期内吞途径快速内吞的证据。  |  Nishimura, Y., et al. 2008. Mol Cancer. 7: 42. PMID: 18492291
  3. 磷酸化EGFR和PI3K/Akt信号激酶在乳腺癌患者的循环肿瘤细胞中表达。  |  Kallergi, G., et al. 2008. Breast Cancer Res. 10: R80. PMID: 18822183
  4. 表皮生长因子受体(EGFR)、磷酸化EGFR、环加氧酶-2的表达与鼻咽癌的临床病理变量和治疗结果之间的相关性。  |  Huang, TL., et al. 2010. Chang Gung Med J. 33: 619-27. PMID: 21199607
  5. 在MET扩增的肺癌中,作为MET异二聚体化伴侣的转磷酸化的EGFR、HER2、HER3和RET的不同作用。  |  Tanizaki, J., et al. 2011. Br J Cancer. 105: 807-13. PMID: 21847121
  6. Rho激酶在人类纤维肉瘤细胞中通过早期/晚期内吞途径调节配体诱导的磷酸化EGFR内吞的新作用。  |  Nishimura, Y., et al. 2011. J Mol Histol. 42: 427-42. PMID: 21847509
  7. 前列腺癌中磷酸化Akt的免疫反应性:与疾病严重程度和结果、Ki67和磷酸化EGFR表达的关系。  |  Hammarsten, P., et al. 2012. PLoS One. 7: e47994. PMID: 23133535
  8. 使用在同一宿主物种中产生的原发性抗体进行磷酸化EGFR和H2AX的双色IHC检测的半抗原-抗半抗原技术。  |  Hagen, J., et al. 2017. Methods Mol Biol. 1554: 155-160. PMID: 28185188
  9. 芹菜素与吉非替尼联合使用,通过抑制EGFR L858R+T790M突变H1975细胞中的HIF-1α、c-Myc、p-EGFR和葡萄糖代谢,阻断自噬通量并诱导细胞凋亡。  |  Chen, Z., et al. 2019. Front Pharmacol. 10: 260. PMID: 30967777
  10. 原位杂交(ISH)与免疫细胞化学(ICC)联合检测A431培养细胞中磷酸化EGFR。  |  Hagen, J., et al. 2023. Methods Mol Biol. 2593: 213-220. PMID: 36513933

订购信息

产品名称产品编号规格价格数量收藏夹

p-EGFR 抗体 (9H2)

sc-57545
50 µg/0.5 ml
CNY2422.00

p-EGFR (9H2): m-IgG Fc BP-HRP 套装

sc-539060
50 µg Ab; 10 µg BP
CNY2715.00

p-EGFR (9H2): m-IgG1 BP-HRP 套装

sc-541235
50 µg Ab; 20 µg BP
CNY2715.00

How much protein do you recommend loading on the gel for Western blot detection using p-EGFR (9H2): sc-57545 monoclonal antibody?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-02-02
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Rated 5 out of 5 by forest from very good antibodyI can detect band! The antibody was used for Western Blot in hepa1c1c7 cells, it works at a concentration of 1: 100 ~ 200.
Date published: 2022-12-07
Rated 4 out of 5 by Giovanni_ from GoodThe antibody was used for Western Blot in breast carcinoma cells, it works at a concentration of 1: 100.
Date published: 2020-10-16
Rated 4 out of 5 by MuGu from Works wellused for WB looking at mouse tissue samples using 1:200. Provides a faint but clear band.
Date published: 2018-03-22
Rated 5 out of 5 by Angelabaik from Great antibodyi got this antibody as free trial size and worked so well on mouse mammary tissue lysates.
Date published: 2017-12-15
Rated 5 out of 5 by Sweetums from Great phospho-detectionGreat, published antibody for p-EGFR detection recommended for use in mouse, rat, human, and canine. Strong bands in WB for phosphorylated samples.
Date published: 2017-01-30
Rated 5 out of 5 by Nina from Working great with dSTORM IF with HeLa andWorking great with dSTORM IF with HeLa and Cos7 cells.
Date published: 2015-03-30
Rated 5 out of 5 by JM from Good Western blot analysis of EGFR phosphorylationGood Western blot analysis of EGFR phosphorylation in non-stimulated, EGF stimulated and pervanadate stimulated A549 whole cell lysates. - SCBT QC
Date published: 2014-02-09
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p-EGFR 抗体 (9H2) is rated 4.8 out of 5 by 8.
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