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p-EGFR Antibody (12A3) - Western Blotting - Image 62884 — p-EGFR 抗体 (12A3): sc-57542. 非刺激 (A), EGF 刺激 (B) 和普伐那酸处理 (C) 的 A549 全细胞裂解液中表皮生长因子受体磷酸化的 Western 印迹分析.

p-EGFR Antibody (12A3) - Western Blotting - Image 62883 — p-EGFR 抗体 (12A3): sc-57542. 对未受刺激 (A), 受 EGF 刺激 (B) 和经普伐那酸处理 (C) 的 Hep G2 全细胞裂解液中的表皮生长因子受体磷酸化进行 Western 印迹分析.

p-EGFR 抗体 (12A3): sc-57542. 非刺激 (A), EGF 刺激 (B) 和普伐那酸处理 (C) 的 A549 全细胞裂解液中表皮生长因子受体磷酸化的 Western 印迹分析.

p-EGFR 抗体 (12A3): sc-57542

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说明书

p-EGFR 抗体 12A3 是小鼠单克隆 IgG₁, 在19篇文献中引用，提供 50 µg/0.5 ml
针对human来源的EGFR磷酸化肽段
推荐用于 mouse, rat 和 human 来源的Tyr 845 phosphorylated EGFR WB 和 IP检测; 与non-phosphorylated EGFR nor with unrelated tyrosine-phosphorylated proteins不交叉反应
看 EGFR (A-10): sc-373746，可查看有标记的 p-EGFR 抗体，包括AC, HRP, FITC, PE, Alexa Fluor^® 488, 594, 647, 680 和 790
m-IgG Fc BP-HRP和 m-IgG₁ BP-HRP是p-EGFR Antibody (12A3) for WB applications. 的首选辅助检测试剂，这些试剂现与p-EGFR Antibody (12A3) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(17)

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p-EGFR 抗体 (12A3) 参考文献:

表皮生长因子受体、EGFRvIII和磷酸化表皮生长因子受体在大肠癌中的共表达、预后意义和预测价值。 | Cunningham, MP., et al. 2005. Int J Oncol. 27: 317-25. PMID: 16010411
表皮生长因子受体（EGFR）和磷酸化表皮生长因子受体在浸润性乳腺癌中的表达。 | Magkou, C., et al. 2008. Breast Cancer Res. 10: R49. PMID: 18522728
酪氨酸1173位点的磷酸化EGFR与口腔鳞状细胞癌预后不良相关。 | Monteiro, L., et al. 2014. Oral Dis. 20: 178-85. PMID: 23464360
胞浆磷酸化EGFR可预测早期阴茎癌患者的复发：回顾性研究。 | Di Lorenzo, G., et al. 2013. J Transl Med. 11: 161. PMID: 23819610
IFIT1和IFIT3促进口腔鳞状细胞癌转移，并通过增强p-EGFR循环促进吉非替尼的抗肿瘤作用。 | Pidugu, VK., et al. 2019. Oncogene. 38: 3232-3247. PMID: 30626937
依鲁替尼的衍生物Ibr-7通过下调p-EGFR使胰腺癌细胞对放射线敏感。 | Tan, B., et al. 2020. Cancer Cell Int. 20: 458. PMID: 32963499
磷酸化表皮生长因子受体（pEGFR T693）作为非功能性垂体腺瘤复发的新预测因子。 | Rai, A., et al. 2021. Front Endocrinol (Lausanne). 12: 708111. PMID: 34295309
雌激素可减轻创伤后骨关节炎的发展，并降低雌性小鼠软骨中的p-EGFR水平。 | Lu, Z., et al. 2022. BMC Musculoskelet Disord. 23: 685. PMID: 35854298
原位杂交（ISH）与免疫组织化学（IHC）联合检测肺癌组织中的EGFR RNA和磷酸化EGFR蛋白。 | Oliver, A., et al. 2023. Methods Mol Biol. 2593: 221-232. PMID: 36513934
西妥昔单抗通过抑制p-EGFR和p-ERK，通过G2/M周期阻滞和DNA修复延迟来增强食管鳞状细胞癌细胞的放射敏感性。 | Zhao, G., et al. 2023. Thorac Cancer. 14: 2127-2138. PMID: 37337933

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	EGFR	Entrez Gene ID: 1956	7p11.2	NM_001346897, NM_001346898, NM_001346899, NM_001346900, NM_001346941, NM_005228, NM_201282, NM_201283, NM_201284	UniProt ID: P00533	211980
Mouse	Egfr	Entrez Gene ID: 13649	11 A2	NM_007912, NM_207655	UniProt ID: Q01279	N/A

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
p-EGFR 抗体 (12A3)	sc-57542	50 µg/0.5 ml	CNY2422.00
p-EGFR (12A3): m-IgG Fc BP-HRP 套装	sc-539059	50 µg Ab; 10 µg BP	CNY2715.00
p-EGFR (12A3): m-IgG₁ BP-HRP 套装	sc-541234	50 µg Ab; 20 µg BP	CNY2715.00

At what dilution should the antibody be used for a western blot (in 5% non-fat dry milk/TBST) ?

Asked by: MmeSprits

Thank you for your question. The recommended starting dilution for this antibody is 1:100 for Western blot applications.

Answered by: Tech Service

Date published: 2020-02-28

Is p-EGFR (12A3): sc-57542 monoclonal antibody specific for the phosphorylated protein, and will not cross-react with the non-phosphorylated form of the protein?

Asked by: jerojero

Yes, p-EGFR (12A3): sc-57542 is specific for p-EGFR phosphorylated at Tyrosine 845. The antibody has been validated through a series Western blot experiments using stimulated, non-stimulated and pervanadate treated lysates to demonstrate phospho-specificity.

Answered by: Technical Support

Date published: 2017-04-05

Rated 5 out of 5 by MuGu from Works great!Highly recommend. used at 1:200 for human and mouse samples and worked great but I recommend longer exposure times.

Date published: 2018-03-22

Rated 5 out of 5 by cjMara from Great for Tyr 845 phosphorylated EGFRThis monoclonal, p-EGFR (12A3): sc-57542, is specific for Tyr 845 phosphorylated EGFR and we induced phosphorylation using both EGF-stimulated, and pervanadate treated A549 and Hep G2 whole cell lysates.

Date published: 2017-02-01

Rated 4 out of 5 by SF from Works well with a little background using EGFWorks well with a little background using EGF-stimulated and pervanadate treated A549 whole cell lysates by WB. - SCBT QC

Date published: 2015-02-09

Rated 4 out of 5 by AB from Good signal strength with a little backgroundGood signal strength with a little background for western blot analysis of EGFR phosphorylation in EGF stimulated and pervanadate treated Hep G2 whole cell lysates.- SCBT QC

Date published: 2014-10-21

p-EGFR 抗体 (12A3) is rated 4.5 out of 5 by 4.

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p-EGFR 抗体 (12A3): sc-57542

p-EGFR 抗体 (12A3) 参考文献:

p-EGFR 抗体 (12A3) 规格

替代产品名称: EGFR

产品克隆名称: 12A3

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁

氨基酸: Tyr 845

抗原表位靶标: p-EGFR

抗原表位种属: human

产品反应性/检测: Tyr 845 phosphorylated EGFR

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP

瓶的信息: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

研究引文: 19篇各种科学出版物的引文

p-EGFR 抗体 (12A3)可选的偶联物/形式: N/A

優選的 EGFR 抗體是 EGFR 抗體 (528): sc-120.