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p-Bad 抗体 (C-10): sc-166932

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说明书
  • p-Bad抗体(C-10)是小鼠单克隆IgG2b κ, 在2篇文献中引用,规格为200 µg/ml
  • 抗-p-Bad 抗体 (C-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Ser 112 phosphorylated Bad
  • 抗p-Bad抗体(C-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Bad (C-10): sc-166932免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是p-Bad Antibody (C-10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与p-Bad Antibody (C-10) 搭配使用(请参阅下面的订购信息)。

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関連項目

p-Bad 抗体(C-10)是一种 IgG2b κ 小鼠单克隆 p-Bad 抗体,可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类来源的 Ser 112 磷酸化 Bad。p-Bad 抗体(C-10)既可以是非偶联的抗 p-Bad 抗体形式,也可以是多种偶联形式的抗 p-Bad 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。BAD 是 Bcl-2 蛋白家族中的促凋亡成员,其在丝氨酸 112 或丝氨酸 136 上的磷酸化被认为是生长因子促进细胞存活所必需且充分的。丝氨酸 155 是蛋白激酶 A (PKA) 和血清诱导激酶的主要磷酸化位点。丝氨酸 155 的磷酸化需要丝氨酸 136 的先前磷酸化,后者会募集 14-3-3 蛋白,然后发挥作用以增加丝氨酸 155 对促存活激酶的可及性。与丝氨酸 112 和丝氨酸 136 一样,丝氨酸 155 的磷酸化会抑制 BAD 的促凋亡功能。丝氨酸 155 的磷酸化会破坏 BAD 与促存活 Bcl-2 蛋白的结合,从而促进细胞存活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p-Bad 抗体 (C-10)

sc-166932
200 µg/ml
RMB2377.00

p-Bad (C-10): m-IgG Fc BP-HRP 套装

sc-525775
200 µg Ab; 10 µg BP
RMB2662.00

p-Bad 抗体 (C-10) AC

sc-166932 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Bad 抗体 (C-10) HRP

sc-166932 HRP
200 µg/ml
RMB2377.00

p-Bad 抗体 (C-10) FITC

sc-166932 FITC
200 µg/ml
RMB2482.00

p-Bad 抗体 (C-10) PE

sc-166932 PE
200 µg/ml
RMB2580.00

p-Bad 抗体 (C-10) Alexa Fluor® 488

sc-166932 AF488
200 µg/ml
RMB2685.00

p-Bad 抗体 (C-10) Alexa Fluor® 546

sc-166932 AF546
200 µg/ml
RMB2685.00

p-Bad 抗体 (C-10) Alexa Fluor® 594

sc-166932 AF594
200 µg/ml
RMB2685.00

p-Bad 抗体 (C-10) Alexa Fluor® 647

sc-166932 AF647
200 µg/ml
RMB2685.00

p-Bad 抗体 (C-10) Alexa Fluor® 680

sc-166932 AF680
200 µg/ml
RMB2685.00

p-Bad 抗体 (C-10) Alexa Fluor® 790

sc-166932 AF790
200 µg/ml
RMB2685.00

p-Bad (C-10) 中和勝肽

sc-166932 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-166932 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Too much bandsI had tested it in C42B and VCaP cell lines.It has too much bands, can't find out which band it is.
Date published: 2017-10-11
Rated 5 out of 5 by from 蛋白表达很清晰Good antibody for western and clean background. I bought it two month ago.
Date published: 2017-08-29
Rated 5 out of 5 by from good antibodyI got a nice double band in westren blot, Thank you!
Date published: 2017-08-25
Rated 5 out of 5 by from ExcellentGreat antibody, works perfectly, got exact band size. Thanks Korey and Santa Cruz .
Date published: 2017-01-07
Rated 5 out of 5 by from Good WB dataGood p-Bad bands in WB, at 1:1000 dilution, with Cal27, scc25 whole cell lysates. Thanks Thanks Santa Cruz for the sample!
Date published: 2016-12-14
Rated 5 out of 5 by from Good Western Blot data of Bad phosphorylationGood Western Blot data of Bad phosphorylation in non-transfected untreated human Bad transfected, PMA treated human Bad transfected and PMA and lambda protein phosphatase treated human Bad transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-10-28
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p-Bad 抗体 (C-10) is rated 4.8 out of 5 by 6.
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