Date published: 2025-9-7
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p-4E-BP1/2/3 Antibody (A-10) - Western Blotting - Image 374935
p-4E-BP1/2/3 抗体 (A-10): sc-271947. MDA-MB-231 (A), K-562 (B), HL-60 (C), F9 (D), NIH/3T3 (E) 和 C6 (F) 全细胞裂解液中 4E-BP1/2/3 磷酸化的 Western 印迹分析.
p-4E-BP1/2/3 抗体 (A-10): sc-271947. MDA-MB-231 (A), K-562 (B), HL-60 (C), F9 (D), NIH/3T3 (E) 和 C6 (F) 全细胞裂解液中 4E-BP1/2/3 磷酸化的 Western 印迹分析.

p-4E-BP1/2/3 抗体 (A-10): sc-271947

4.3(9)
寫評論提問

说明书
  • p-4E-BP1/2/3(A-10)是一种小鼠单克隆 IgG2a κ，在7篇出版物中被引用，在200 µg/ml处提供
  • 建议在mouse, rat和human中用WB, IP, IF和ELISA检测p-4E-BP1/2/3(A-10)。
  • 抗p-4E-BP1/2/3抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-4E-BP1/2/3 (A-10): sc-271947的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是p-4E-BP1/2/3 Antibody (A-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p-4E-BP1/2/3 Antibody (A-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加4E-BP抗体，包括4E-BP1, 4E-BP2和4E-BP3

p-4E-BP1/2/3抗体（A-10）是一种IgG2a κ小鼠单克隆p-4E-BP1/2/3抗体（也称为p-4E-BP1/2/3抗体或4EBP1、4EBP2、4EB3抗体），可通过WB、IP、IF和ELISA检测苏氨酸45磷酸化的4E-BP1以及相应磷酸化的小鼠、大鼠和人类的4E-BP2和4E-BP3。p-4E-BP1/2/3抗体（A-10）既可以是非偶联的抗p-4E-BP1/2/3抗体形式，也可以是多种偶联形式的抗p-4E-BP1/2/3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。多亚基真核翻译起始因子（eIF）4F将40S核糖体亚基募集到mRNA的5'端。eIF4F亚基eIF4E直接与mRNA 5'帽结构相互作用。eIF4F复合物的组装被一系列阻遏多肽抑制，即eIF4E结合蛋白（4E-BPs）。4E-BP1（也称为PHAS-1）通常与eIF4E结合，抑制帽依赖性翻译。4E-BP1的超磷酸化破坏了这种结合，激活了帽依赖性翻译。PI3激酶/Akt通路和FRAP/mTOR激酶调节4E-BP1。4E-BP1在体内被多个残基磷酸化，FRAP/mTOR在人4E-BP1的苏氨酸37和苏氨酸46上的磷酸化可能使其随后在包括丝氨酸65和苏氨酸70的位点上进行磷酸化。相应的大鼠残基包括苏氨酸36、苏氨酸45、丝氨酸64和苏氨酸69。在体外，4E-BP1还响应胰岛素水平的增加而在人丝氨酸112（大鼠丝氨酸111）处被共济失调毛细血管扩张（ATM）磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-4E-BP1/2/3 抗体 (A-10) 参考文献:

  1. 4E-BP3调节eIF4E介导的核mRNA出口，并与复制蛋白A2相互作用。  |  Chen, CC., et al. 2012. FEBS Lett. 586: 2260-6. PMID: 22684010
  2. 尽管在植入过程中猪子宫组织中的4E-BP1降解，但eIF4E抑制蛋白4E-BP2仅被截短。  |  Wollenhaupt, K., et al. 2012. Mol Reprod Dev. 79: 767-76. PMID: 22968905
  3. 4E-BP3 的转录诱导延长了翻译抑制。  |  Tsukumo, Y., et al. 2016. Cell Cycle. 15: 3325-3326. PMID: 27565030
  4. 磷酸化4E-BP1与结直肠癌的肿瘤进展和不良预后相关。  |  Chen, Y., et al. 2017. Neoplasma. 64: 787-794. PMID: 28592131
  5. 4E-BP1和4E-BP2对Ccl5和Cxcl10的翻译抑制抑制了小鼠巨噬细胞诱导活化T细胞迁移的能力。  |  William, M., et al. 2019. Eur J Immunol. 49: 1200-1212. PMID: 31032899
  6. 磷酸化翻译抑制因子 4E-BP2 及其突变体的多通道能量景观。  |  Kang, W., et al. 2020. J Chem Theory Comput. 16: 800-810. PMID: 31774674
  7. 多位点磷酸化与结合改变了4E-BP2蛋白的构象动力学。  |  Smyth, S., et al. 2022. Biophys J. 121: 3049-3060. PMID: 35841142
  8. 二氢嘧啶酶相关蛋白2是4E-BP2和eIF4E之间结合的新伙伴 与脑缺血后神经元死亡有关  |  Martínez-Alonso, E., et al. 2023. Int J Mol Sci. 24: PMID: 37175950
  9. 通过综合建模和聚类确定 4E-BP2 蛋白的结构倾向性  |  Tsangaris, TE., et al. 2023. J Phys Chem B. 127: 7472-7486. PMID: 37595014
  10. 真核生物启动因子 4E 结合蛋白家族的新成员 4E-BP3  |  Poulin, F., et al. 1998. J Biol Chem. 273: 14002-7. PMID: 9593750

订购信息

产品名称产品编号规格价格数量收藏夹

p-4E-BP1/2/3 抗体 (A-10)

sc-271947
200 µg/ml
$316.00

p-4E-BP1/2/3 (A-10): m-IgG Fc BP-HRP 套装

sc-529291
200 µg Ab; 10 µg BP
$354.00

p-4E-BP1/2/3 (A-10): m-IgGκ BP-HRP 套装

sc-522107
200 µg Ab, 40 µg BP
$354.00

p-4E-BP1/2/3 (A-10): m-IgG2a BP-HRP 套装

sc-547295
200 µg Ab; 10 µg BP
$354.00

p-4E-BP1/2/3 抗体 (A-10) AC

sc-271947 AC
500 µg/ml, 25% agarose
$416.00

p-4E-BP1/2/3 抗体 (A-10) HRP

sc-271947 HRP
200 µg/ml
$316.00

p-4E-BP1/2/3 抗体 (A-10) FITC

sc-271947 FITC
200 µg/ml
$330.00

p-4E-BP1/2/3 抗体 (A-10) PE

sc-271947 PE
200 µg/ml
$343.00

p-4E-BP1/2/3 抗体 (A-10) Alexa Fluor® 488

sc-271947 AF488
200 µg/ml
$357.00

p-4E-BP1/2/3 抗体 (A-10) Alexa Fluor® 546

sc-271947 AF546
200 µg/ml
$357.00

p-4E-BP1/2/3 抗体 (A-10) Alexa Fluor® 594

sc-271947 AF594
200 µg/ml
$357.00

p-4E-BP1/2/3 抗体 (A-10) Alexa Fluor® 647

sc-271947 AF647
200 µg/ml
$357.00

p-4E-BP1/2/3 抗体 (A-10) Alexa Fluor® 680

sc-271947 AF680
200 µg/ml
$357.00

p-4E-BP1/2/3 抗体 (A-10) Alexa Fluor® 790

sc-271947 AF790
200 µg/ml
$357.00

p-4E-BP1/2/3 (A-10) 中和勝肽

sc-271947 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-271947 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Good for WBWork fine in western blot analysis in A498 ccRCC cells
Date published: 2024-11-26
Rated 1 out of 5 by from ちょっと残念ラット骨格筋組織ライゼートでWBしましたが、ラダー状のバンドが出てしまい、同定不可能でした。IP-WBの必要があります。
Date published: 2023-05-18
Rated 5 out of 5 by from Very good antibodyReceived a sample and used it to probe by western blotting lysates of activated human leukocytes. Antibody worked very well. We detected two strong bands, and one faint third band. The same bands were also detected using the 4E-BP1 (sc-81149) antibody.
Date published: 2020-03-11
Rated 4 out of 5 by from hardit's hard for me to detect this protein by using this antibody.
Date published: 2017-10-05
Rated 5 out of 5 by from western bloti asked the sample for 3T3-l1 cell western blot , work very well, clear band.
Date published: 2017-09-27
Rated 5 out of 5 by from Good resultsThis antibody gave satisfying results in wetern blot.
Date published: 2017-09-05
Rated 5 out of 5 by from ExcellentThis antibody works well when we use 1:500 dilution in WB, and our results shows a beautiful brand.
Date published: 2017-03-21
Rated 5 out of 5 by from Very potent and specific antibodyThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of 4E-BP1 in overnight serum-starved human A431 cells treated for 5 minutes with EGF on Western blots, and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2017-03-14
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p-4E-BP1/2/3 抗体 (A-10) is rated 4.3 out of 5 by 9.
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