Date published: 2025-9-11
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Oval Cell Marker Antibody (OV-6) - Western Blotting - Image 411230
Oval Cell Marker Antibody (OV-6): sc-101863. Western blot analysis of Oval Cell Marker expression in HeLa whole cell lysate. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.
Oval Cell Marker Antibody (OV-6): sc-101863. Western blot analysis of Oval Cell Marker expression in HeLa whole cell lysate. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.

Oval Cell Marker 抗体 (OV-6): sc-101863

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说明书
  • Oval Cell Marker 抗体 OV-6 是小鼠单克隆 IgG1 κ，Oval Cell Marker抗体, 在8篇文献中引用，规格为200 µg/ml
  • 针对rat来源的经致癌物质处理的结节性肝细胞
  • Oval Cell Marker 抗体 (OV-6) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 OV-6 antigen
  • 抗Oval Cell Marker抗体（OV-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Oval Cell Marker (OV-6): sc-101863的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Oval Cell Marker Antibody (OV-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Oval Cell Marker Antibody (OV-6) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

Oval细胞标记物抗体（OV-6）是一种IgG1 κ型小鼠单克隆Oval细胞标记物抗体（也称为Oval细胞标记物抗体），可通过IF和FCM检测到小鼠、大鼠和人类来源的Oval细胞标记物蛋白。 Oval细胞标记物抗体（OV-6）既有非偶联型抗Oval细胞标记物抗体形式，也有多种偶联形式的抗Oval细胞标记物抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。干细胞几乎存在于所有多细胞生物中，其特点是具有自我更新和分化成多种不同细胞类型的能力。干细胞主要有两种类型，即成体干细胞和胚胎干细胞。成体干细胞在组织修复和器官维持中发挥重要作用，而胚胎干细胞则存在于囊胚中，可以分化形成任何和所有胚胎组织。由于干细胞具有独特的分化成任何细胞类型的能力，因此在治疗人类疾病方面特别受关注，包括癌症、肌肉损伤、脊髓损伤、帕金森病、肌萎缩性侧索硬化症和器官再生。Oval细胞是肝干细胞（肝脏特异性干细胞），可分化成多种细胞类型，参与肝脏发育以及慢性肝病的发病机理。Oval细胞标记物，也称为OV-6，可用于检测特定样品中Oval细胞的存在。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Oval Cell Marker 抗体 (OV-6) 参考文献:

  1. 干细胞的潜力  |  Pfendler, KC. and Kawase, E. 2003. Obstet Gynecol Surv. 58: 197-208. PMID: 12612460
  2. 胚胎干细胞作为治疗帕金森病的细胞来源。  |  Fukuda, H. and Takahashi, J. 2005. Expert Opin Biol Ther. 5: 1273-80. PMID: 16197334
  3. 干细胞--临床更新。  |  Tuch, BE. 2006. Aust Fam Physician. 35: 719-21. PMID: 16969445
  4. 胚胎干细胞移植：细胞替代疗法和再生药物的潜在适用性。  |  Wu, DC., et al. 2007. Front Biosci. 12: 4525-35. PMID: 17485394
  5. 回顾：利用成体干细胞进行活组织体外工程。  |  Barrilleaux, B., et al. 2006. Tissue Eng. 12: 3007-19. PMID: 17518617
  6. 治疗帕金森病的干细胞疗法。  |  Takahashi, J. 2007. Expert Rev Neurother. 7: 667-75. PMID: 17563250
  7. 干细胞：神奇的酿造  |  Rossant, J. 2007. Nature. 448: 260-2. PMID: 17637646
  8. 干细胞移植：治疗帕金森病的一种前景广阔的疗法。  |  Wang, Y., et al. 2007. J Neuroimmune Pharmacol. 2: 243-50. PMID: 18040857
  9. ENGOT-ov-6/TRINOVA-2：聚乙二醇化脂质体阿霉素联合trebananib或安慰剂治疗部分对铂敏感或耐药的复发性卵巢癌患者的随机、双盲、三期研究。  |  Marth, C., et al. 2017. Eur J Cancer. 70: 111-121. PMID: 27914241
  10. 肝细胞癌中高迁移率组蛋白盒1和OV-6表达组合的预后意义。  |  Zhu, J., et al. 2018. Sci China Life Sci. 61: 912-923. PMID: 29441453
  11. 人类胎儿和患病儿童肝脏中假定的祖细胞标记物OV-6的免疫定位。  |  Crosby, HA., et al. 1998. Hepatology. 28: 980-5. PMID: 9755234
  12. 从人类胚泡中提取的胚胎干细胞系。  |  Thomson, JA., et al. 1998. Science. 282: 1145-7. PMID: 9804556

订购信息

产品名称产品编号规格价格数量收藏夹

Oval Cell Marker 抗体 (OV-6)

sc-101863
200 µg/ml
RMB2377.00

Oval Cell Marker (OV-6): m-IgG Fc BP-HRP 套装

sc-528725
200 µg Ab; 10 µg BP
RMB2662.00

Oval Cell Marker (OV-6): m-IgGκ BP-HRP 套装

sc-521203
200 µg Ab, 40 µg BP
RMB2662.00

Oval Cell Marker (OV-6): m-IgG1 BP-HRP 套装

sc-543093
200 µg Ab; 20 µg BP
RMB2662.00

Oval Cell Marker 抗体 (OV-6) AC

sc-101863 AC
500 µg/ml, 25% agarose
RMB3129.00

Oval Cell Marker 抗体 (OV-6) HRP

sc-101863 HRP
200 µg/ml
RMB2377.00

Oval Cell Marker 抗体 (OV-6) FITC

sc-101863 FITC
200 µg/ml
RMB2482.00

Oval Cell Marker 抗体 (OV-6) PE

sc-101863 PE
200 µg/ml
RMB2580.00

Oval Cell Marker 抗体 (OV-6) Alexa Fluor® 488

sc-101863 AF488
200 µg/ml
RMB2685.00

Oval Cell Marker 抗体 (OV-6) Alexa Fluor® 546

sc-101863 AF546
200 µg/ml
RMB2685.00

Oval Cell Marker 抗体 (OV-6) Alexa Fluor® 594

sc-101863 AF594
200 µg/ml
RMB2685.00

Oval Cell Marker 抗体 (OV-6) Alexa Fluor® 647

sc-101863 AF647
200 µg/ml
RMB2685.00

Oval Cell Marker 抗体 (OV-6) Alexa Fluor® 680

sc-101863 AF680
200 µg/ml
RMB2685.00

Oval Cell Marker 抗体 (OV-6) Alexa Fluor® 790

sc-101863 AF790
200 µg/ml
RMB2685.00

Who knows which gene transcript OV6? I did not find the gene symbol OV6 in gene expression profiles

Asked by: drwang
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-10-06

I'm getting unspecificity from this in mouse liver samples. I have tried diluting it more, different antigen retrievals and longer blocking times. Had a sample of this before which worked fine. Anyone know how to troubleshoot this?

Asked by: Sian
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-09-22

Heloo. Please, write me what material can be used as a positive control of the product Oval Cell Marker Antibody (OV-6): sc-101863. Thanks.

Asked by: Nino
Thank you for your question. Liver tissues and cells (hepatic samples) are considered the best positive controls. This antibody is specific for bile ducts, oval cells, some nodular hepatocytes and hepatomas.
Answered by: Tech Service
Date published: 2019-10-03

Which antigen retrieval technique is needed for IHC-P ? thanks

Asked by: SophiePaquet
This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections, or for IHC(P). This antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-06-16

What negative control should be used when using the PE conjugated version of this antibody (sc-101863 PE) in flow cytometry?

Asked by: TinTin
Great question! We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. So if you are using sc-101863 PE, then you would want to use normal mouse IgG1 PE, sc-2866.
Answered by: Technical Support 8
Date published: 2021-02-18
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Rated 5 out of 5 by from Published for IF and IHCThis mouse monoclonal Oval Cell Marker Antibody (OV-6) is featured in recent publications for use in IHC and IF research applications.
Date published: 2017-01-21
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Oval Cell Marker 抗体 (OV-6) is rated 4.5 out of 5 by 2.
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