Date published: 2025-9-8
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Orexin-A Antibody (KK09) - Western Blotting - Image 404626
Orexin-A 抗体 (KK09): sc-80263. 人重组 Orexin-A/B 融合蛋白的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP (Cruz Marker): sc-516102-CM.
Orexin-A Antibody (KK09) - Western Blotting - Image 404626
Orexin-A 抗体 (KK09): sc-80263. 人重组 Orexin-A/B 融合蛋白的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP (Cruz Marker): sc-516102-CM.
Orexin-A 抗体 (KK09): sc-80263. 人重组 Orexin-A/B 融合蛋白的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP (Cruz Marker): sc-516102-CM.

Orexin-A 抗体 (KK09): sc-80263

4.5(4)
寫評論提問

说明书
  • Orexin-A 抗体 KK09 是小鼠单克隆 IgG1 κ，Orexin-A抗体, 在22篇文献中引用，规格为50 µg/0.5 ml
  • 针对氨基酸序列34-66内的合成肽，在Orexin-A的内部区域human起源
  • 推荐用于 mouse, rat 和 human 来源的Orexin-A WB, IP, IF 和 IHC(P)检测; 不会与 Orexin-B产生交联反应
  • 关于如何获取Orexin-A (KK09): sc-80263的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Orexin-A 抗体 (KK09) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Orexin-A 抗体 (KK09) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Orexin 抗体包括 Orexin R-1, Orexin R-2 和 Orexin-A

Orexin-A抗体（KK09）是一种小鼠单克隆IgG1抗体，旨在通过蛋白质印迹（WB）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测小鼠、大鼠和人类中的Orexin-A。抗Orexin-A抗体（KK09）靶向与人类Orexin-A内部区域中34-66个氨基酸相对应的合成肽，确保选择性结合，而不会与Orexin-B发生交叉反应。Orexin-A，也称为Hypocretin-1、HCRT和PPOX，是一种重要的下丘脑神经肽，由前体prepro-orexin衍生而来，通过调节温度、口渴和饥饿来维持体内平衡。Orexin-A通过与Orexin受体-1和Orexin受体-2结合而发挥功效，这两种受体属于G蛋白偶联受体，参与内分泌系统和神经系统的整合。Orexin-A主要定位于被称为"进食中枢"的下丘脑外侧部的神经元，在禁食期间表达量会升高，这凸显了其在控制进食行为的中枢反馈机制中的重要性。此外，Orexin-A可促进GABA和谷氨酸等神经递质的释放，从而促进下丘脑区域的突触活动。Orexin-A单克隆抗体（KK09）是研究人员研究食欲素在能量平衡、睡眠调节和神经退行性疾病中的生理和病理作用的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Orexin-A 抗体 (KK09) 参考文献:

  1. Orexin-A在高血压和肥胖症中的作用。  |  Imperatore, R., et al. 2017. Curr Hypertens Rep. 19: 34. PMID: 28353077
  2. 双向肠道-大脑交流：Orexin-A的作用。  |  Mediavilla, C. 2020. Neurochem Int. 141: 104882. PMID: 33068686
  3. 嗜睡症中食欲素-A的测量：稳定性研究以及LC-MS/MS和免疫分析的比较。  |  Lindström, M., et al. 2021. Clin Biochem. 90: 34-39. PMID: 33539807
  4. 改良的苏安唑仑汤通过调节Orexin-A和HPA轴稳态，对失眠小鼠模型产生镇静作用。  |  Dong, YJ., et al. 2021. Biomed Pharmacother. 143: 112141. PMID: 34509822
  5. 路易体病患者中的食欲素-A：系统评价和荟萃分析。  |  Gan, J., et al. 2021. Front Endocrinol (Lausanne). 12: 765701. PMID: 34867809
  6. Orexin A与癫痫发作的关系。  |  Arslan, GA., et al. 2022. Epilepsy Res. 184: 106972. PMID: 35772324
  7. Orexin A通过JNK介导的自动降解抑制巨噬细胞活化，从而减轻LPS引起的急性肺损伤。  |  Nie, Y., et al. 2023. Int Immunopharmacol. 124: 111018. PMID: 37801969
  8. 食欲素-A对小鼠败血症相关性脑病的作用。  |  Guo, J., et al. 2024. J Neuroinflammation. 21: 131. PMID: 38760784
  9. 促肾上腺皮质激素：具有神经兴奋活性的下丘脑特异性肽。  |  de Lecea, L., et al. 1998. Proc Natl Acad Sci U S A. 95: 322-7. PMID: 9419374
  10. 奥曲肽和奥曲肽受体：调节摄食行为的下丘脑神经肽和 G 蛋白偶联受体家族。  |  Sakurai, T., et al. 1998. Cell. 92: 573-85. PMID: 9491897
  11. 催乳素：新发现的下丘脑食物摄入调节器家族。  |  Wolf, G. 1998. Nutr Rev. 56: 172-3. PMID: 9656726
  12. 一种新的下丘脑多肽--视网膜下视素/orexin--对神经内分泌神经元的突触前和突触后作用及调制。  |  van den Pol, AN., et al. 1998. J Neurosci. 18: 7962-71. PMID: 9742163

订购信息

产品名称产品编号规格价格数量收藏夹

Orexin-A 抗体 (KK09)

sc-80263
50 µg/0.5 ml
RMB2377.00

Orexin-A (KK09): m-IgG Fc BP-HRP 套装

sc-539559
50 µg Ab; 10 µg BP
RMB2662.00

Orexin-A (KK09): m-IgG1 BP-HRP 套装

sc-541556
50 µg Ab; 20 µg BP
RMB2662.00

(KK09): m-IgGκ BP-HRP 套装

sc-551569
50 µg Ab; 40 µg BP
RMB2662.00

How can this be specific for Orexin A when the pre-pro hormone Ox A/B is simply cleaved to produce mature Ox A and Ox B? Won't an Ox A antibody also react with the pre-pro hormone and therefore also show Ox B (and vice versa for any Ox B antibody)?

Asked by: Doug V
Thank you for your question. We understand where you are coming from and we prefer to have a discussion by phone so we can cover all of the intricate details. Please give us a call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-08-09

Re: Anti-Orexin-A Antibody (KK09): sc-80263 Does this product come from the 8G22 mouse hybridoma clone?

Asked by: NickolasU
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-09-07

Can this antibody be used in avian species for IF purpose

Asked by: undergrad
This antibody has not been tested on Avian species.
Answered by: Tech Sales
Date published: 2021-04-29

What is the recommended fixation method for immunofluorescence staining with Orexin-A (KK09): sc-80263 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-10-16
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Rated 5 out of 5 by from Great expression with limited background stainingOur lab tested this antibody on free floating rat brain sections. We did a DAB stain (1:2000) and fluorescent stain (1:1000). We had very good expression in later hypothalamus and almost no background staining.
Date published: 2024-05-24
Rated 5 out of 5 by from Cited Monoclonal AntibodyOrexin-A Antibody (KK09) has been cited in multiple publications for immunostaining
Date published: 2017-01-21
Rated 4 out of 5 by from Antibody was published for use in IHC (pfa)Antibody was published for use in IHC (pfa) on rat brains - SCBT Publication Review
Date published: 2015-04-26
Rated 4 out of 5 by from Good publishable data in IF using rat ratGood publishable data in IF using rat rat lateral hypothalamic area -SCBT Publication Review
Date published: 2014-04-14
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Orexin-A 抗体 (KK09) is rated 4.5 out of 5 by 4.
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