Date published: 2025-9-8

021-6093-6350

SCBT Portrait Logo
Seach Input

ORC6 抗体 (3A4): sc-32735

4.3(6)
寫評論提問

说明书
  • ORC6 抗体 3A4 是大鼠单克隆 IgG2aORC6抗体, 在10篇文献中引用,规格为200 µg/ml
  • 针对human重组ORC6蛋白的抗原性氨基酸
  • ORC6 抗体 (3A4) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 ORC6
  • 抗ORC6抗体(3A4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-32735 X, 200 µg/0.1 ml)
  • 关于如何获取ORC6 (3A4): sc-32735免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成ORC6 抗体 (3A4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

関連項目

ORC6抗体(3A4)是一种鼠单克隆IgG2a抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠和人类来源的ORC6蛋白。抗ORC6抗体(3A4)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。ORC6是起源识别复合物(ORC)的重要组成部分,而ORC对于DNA复制的启动至关重要。ORC6在形成复制前复合物方面发挥着关键作用,而复制前复合物对于准确、及时的基因组复制必不可少。ORC6与其他ORC亚基结合到特定的复制起源,作为其他启动因子组装的支架。这种结合调节细胞周期,特别是在G1-S转换期间,细胞准备进行DNA合成。ORC6存在于增殖细胞核中,包括睾丸等组织,这表明其在细胞增殖和基因组稳定性方面的重要性。ORC6在整个细胞周期中与其他ORC蛋白一起表达,可确保DNA复制的高效性和准确性,防止潜在的基因组不稳定,从而避免癌症等疾病的发生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ORC6 抗体 (3A4) 参考文献:

  1. Orc1蛋白的选择性不稳定性是哺乳动物在M-G(1)转变过程中缺乏功能性起源识别复合体的原因。  |  Natale, DA., et al. 2000. EMBO J. 19: 2728-38. PMID: 10835370
  2. 人类 ORC6 同源物的鉴定和特征描述。  |  Dhar, SK. and Dutta, A. 2000. J Biol Chem. 275: 34983-8. PMID: 10945994
  3. 人类起源识别复合体(ORC)亚基在非增殖细胞中表达,并与非 ORC 蛋白缔合作用。  |  Thome, KC., et al. 2000. J Biol Chem. 275: 35233-41. PMID: 10954718
  4. 细胞周期中人类起源识别复合体, cdc6 和迷你染色体维护蛋白的染色质关联:有丝分裂后期复制前复合体的组装。  |  Méndez, J. and Stillman, B. 2000. Mol Cell Biol. 20: 8602-12. PMID: 11046155
  5. 人类起源识别复合蛋白 1 在 HeLa 细胞的 S 期从染色质中解离。  |  Kreitz, S., et al. 2001. J Biol Chem. 276: 6337-42. PMID: 11102449
  6. 人类起源识别复合体的组装。  |  Vashee, S., et al. 2001. J Biol Chem. 276: 26666-73. PMID: 11323433
  7. 人类起源识别综合体的结构。  |  Dhar, SK., et al. 2001. J Biol Chem. 276: 29067-71. PMID: 11395502
  8. 鉴定人类复制起源识别复合体成员 HsORC4。  |  Quintana, DG., et al. 1997. J Biol Chem. 272: 28247-51. PMID: 9353276

订购信息

产品名称产品编号规格价格数量收藏夹

ORC6 抗体 (3A4)

sc-32735
200 µg/ml
RMB2377.00

ORC6 抗体 (3A4) X

sc-32735 X
200 µg/0.1 ml
RMB2377.00

ORC6 抗体 (3A4) AC

sc-32735 AC
500 µg/ml, 25% agarose
RMB3129.00

ORC6 抗体 (3A4) HRP

sc-32735 HRP
200 µg/ml
RMB2377.00

ORC6 抗体 (3A4) FITC

sc-32735 FITC
200 µg/ml
RMB2482.00

ORC6 抗体 (3A4) PE

sc-32735 PE
200 µg/ml
RMB2580.00

ORC6 抗体 (3A4) Alexa Fluor® 488

sc-32735 AF488
200 µg/ml
RMB2685.00

ORC6 抗体 (3A4) Alexa Fluor® 546

sc-32735 AF546
200 µg/ml
RMB2685.00

ORC6 抗体 (3A4) Alexa Fluor® 594

sc-32735 AF594
200 µg/ml
RMB2685.00

ORC6 抗体 (3A4) Alexa Fluor® 647

sc-32735 AF647
200 µg/ml
RMB2685.00

ORC6 抗体 (3A4) Alexa Fluor® 680

sc-32735 AF680
200 µg/ml
RMB2685.00

ORC6 抗体 (3A4) Alexa Fluor® 790

sc-32735 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-32735 X be used instead of the standard form sc-32735?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_32735, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 107ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good antibodyAntibody works well for WB. It gives strong signal at dilution 1:3000 whole cell lysates of HCT116 cells. Protein was detected around 30kDa.
Date published: 2018-07-23
Rated 4 out of 5 by from Publishable data in ChIP using human HeLaPublishable data in ChIP using human HeLa, NSF,WI38 and WI38 VA13 2RA cell lines. -SCBT Publication Review
Date published: 2015-06-23
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear stainingExceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-05-16
Rated 4 out of 5 by from Publishable data in CoIP using human breastPublishable data in CoIP using human breast (MCF10A, MCF10AT, and MCF10AC1a cells) -SCBT Publication Review
Date published: 2015-03-21
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-01-19
Rated 4 out of 5 by from Nice bands seen in WB using UNice bands seen in WB using U-2 OS, SW480 and F9 whole cell lysates. -SCBT QC
Date published: 2015-01-04
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_32735, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
ORC6 抗体 (3A4) is rated 4.3 out of 5 by 6.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_32735, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT