Date published: 2025-9-8

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ORC1 抗体 (7A7): sc-23887

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说明书
  • ORC1 抗体 7A7 是大鼠单克隆 IgG1ORC1抗体, 在7篇文献中引用,规格为200 µg/ml
  • 针对部分纯化的His标签细菌表达融合蛋白(对应于human ORC1
  • ORC1 抗体 (7A7) 推荐用于 WB 和 IP,检测mouse, rat 和human 来源的 ORC1
  • 抗ORC1抗体(7A7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-23887 X, 200 µg/0.1 ml)
  • 关于如何获取ORC1 (7A7): sc-23887免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成ORC1 抗体 (7A7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

ORC1抗体(7A7)是一种鼠单克隆IgG1抗体,可通过蛋白质印迹(WB)和免疫沉淀(IP)检测小鼠、大鼠和人类来源的ORC1蛋白。ORC1 (7A7) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ORC1在DNA复制的启动中起着关键作用,而DNA复制对于细胞分裂和基因组完整性的维持至关重要。ORC1是起源识别复合物(ORC)的关键组成部分,它与复制起点处的特定DNA序列结合,作为组装其他启动因子所需的支架,这些因子对于形成复制前复合物是必不可少的。ORC1的正常运作可确保DNA复制在细胞周期的正确时间准确进行。ORC1在增殖细胞(包括睾丸等组织中的细胞)的细胞核中表达,ORC1与其他ORC亚基(尤其是ORC2)的相互作用对于ORC复合物在整个细胞周期中的稳定性和功能性至关重要。ORC蛋白(包括ORC1、ORC2、ORC3、ORC4、ORC5和ORC6)的动态组装凸显了它们在调节G1-S转换中的重要性,而G1-S转换是细胞周期进展的关键阶段。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ORC1 抗体 (7A7) 参考文献:

  1. Orc1蛋白的选择性不稳定性是哺乳动物在M-G(1)转变过程中缺乏功能性起源识别复合体的原因。  |  Natale, DA., et al. 2000. EMBO J. 19: 2728-38. PMID: 10835370
  2. 人类 ORC6 同源物的鉴定和特征描述。  |  Dhar, SK. and Dutta, A. 2000. J Biol Chem. 275: 34983-8. PMID: 10945994
  3. 人类起源识别复合体(ORC)亚基在非增殖细胞中表达,并与非 ORC 蛋白缔合作用。  |  Thome, KC., et al. 2000. J Biol Chem. 275: 35233-41. PMID: 10954718
  4. 细胞周期中人类起源识别复合体, cdc6 和迷你染色体维护蛋白的染色质关联:有丝分裂后期复制前复合体的组装。  |  Méndez, J. and Stillman, B. 2000. Mol Cell Biol. 20: 8602-12. PMID: 11046155
  5. 人类起源识别复合体的组装。  |  Vashee, S., et al. 2001. J Biol Chem. 276: 26666-73. PMID: 11323433
  6. 人类起源识别综合体的结构。  |  Dhar, SK., et al. 2001. J Biol Chem. 276: 29067-71. PMID: 11395502
  7. 鉴定人类复制起源识别复合体成员 HsORC4。  |  Quintana, DG., et al. 1997. J Biol Chem. 272: 28247-51. PMID: 9353276
  8. 人类起源识别复合蛋白 1 在 HeLa 细胞的 S 期从染色质中解离。  |  Kreitz, S., et al. 2001. J Biol Chem. 276: 6337-42. PMID: 11102449

订购信息

产品名称产品编号规格价格数量收藏夹

ORC1 抗体 (7A7)

sc-23887
200 µg/ml
RMB2377.00

ORC1 抗体 (7A7) X

sc-23887 X
200 µg/0.1 ml
RMB2377.00

ORC1 抗体 (7A7) AC

sc-23887 AC
500 µg/ml, 25% agarose
RMB3129.00

ORC1 抗体 (7A7) HRP

sc-23887 HRP
200 µg/ml
RMB2377.00

ORC1 抗体 (7A7) FITC

sc-23887 FITC
200 µg/ml
RMB2482.00

ORC1 抗体 (7A7) PE

sc-23887 PE
200 µg/ml
RMB2580.00

ORC1 抗体 (7A7) Alexa Fluor® 488

sc-23887 AF488
200 µg/ml
RMB2685.00

ORC1 抗体 (7A7) Alexa Fluor® 546

sc-23887 AF546
200 µg/ml
RMB2685.00

ORC1 抗体 (7A7) Alexa Fluor® 594

sc-23887 AF594
200 µg/ml
RMB2685.00

ORC1 抗体 (7A7) Alexa Fluor® 647

sc-23887 AF647
200 µg/ml
RMB2685.00

ORC1 抗体 (7A7) Alexa Fluor® 680

sc-23887 AF680
200 µg/ml
RMB2685.00

ORC1 抗体 (7A7) Alexa Fluor® 790

sc-23887 AF790
200 µg/ml
RMB2685.00

When should the TransCruz reagent sc-23887 X be used instead of the standard form sc-23887?

Asked by: Germaine
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good for westernDetected ORC1 in mouse tissue samples by western (at 1:1,000 dilution)
Date published: 2017-09-29
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using Ramos nuclear extract. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from Publishable WB and CoIP data with human breastPublishable WB and CoIP data with human breast (MCF10A, MCF10AT, and MCF10AC1a cells) - SCBT Publication Review
Date published: 2015-03-17
Rated 5 out of 5 by from Produced a clear band with HeLa nuclear extractProduced a clear band with HeLa nuclear extract. -SCBT QC
Date published: 2015-02-26
Rated 4 out of 5 by from WB and EMSA publishable data with human HeLaWB and EMSA publishable data with human HeLa, NSF, WI38 and WI38 VA13 2RA - SCBT Publication Review
Date published: 2013-07-14
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ORC1 抗体 (7A7) is rated 4.4 out of 5 by 5.
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