Date published: 2025-9-6
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osteopontin/OPN/SPP1 Antibody (AKm2A1) - Immunohistochemistry - Image 353913
OPN 抗体 (AKm2A1): sc-21742. 福尔马林固定, 石蜡包埋的人胆囊组织的免疫过氧化物酶染色显示腺细胞的胞浆和微弱的核染色.
OPN 抗体 (AKm2A1): sc-21742. 福尔马林固定, 石蜡包埋的人胆囊组织的免疫过氧化物酶染色显示腺细胞的胞浆和微弱的核染色.

osteopontin/OPN/SPP1 抗体 (AKm2A1): sc-21742

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寫評論提問

说明书
  • osteopontin/OPN/SPP1 抗体 AKm2A1 是小鼠单克隆 IgG1 κ，osteopontin/OPN/SPP1抗体, 在369篇文献中引用，规格为200 µg/ml
  • 针对mouse重组骨桥蛋白
  • osteopontin/OPN/SPP1 抗体 (AKm2A1) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 OPN
  • 抗osteopontin/OPN/SPP1抗体（AKm2A1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取osteopontin/OPN/SPP1 (AKm2A1): sc-21742的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 osteopontin/OPN/SPP1 抗体 (AKm2A1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与osteopontin/OPN/SPP1 抗体 (AKm2A1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

OPN抗体（AKm2A1）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类样本中的骨桥蛋白（OPN）。抗骨桥蛋白抗体（AKm2A1）有非结合型和结合型两种形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。骨桥蛋白（Osteopontin）又称骨唾液蛋白1、尿结石蛋白、SPP1、Eta-1、肾桥蛋白和尿桥蛋白，是一种细胞外基质细胞粘附磷糖蛋白，在各种生物过程中发挥着至关重要的作用。OPN的翻译后修饰（如磷酸化与糖基化）会显著影响分子重量和功能特性，使其能够与特定受体和蛋白质相互作用，从而调节细胞粘附、迁移和免疫反应。OPN单克隆抗体（AKm2A1）有助于研究人员研究这种主要由成骨细胞产生并在大脑和肾脏合成的蛋白质，从而证明其在骨骼和非骨骼组织中的重要性。OPN的多种功能因翻译后修饰的结构变化而增强，凸显了其在健康和疾病中的重要性，使得OPN（AKm2A1）抗体对于研究各种生物学背景具有价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

osteopontin/OPN/SPP1 抗体 (AKm2A1) 参考文献:

  1. 骨化蛋白是转谷氨酰胺酶和因子 XIII 活性的底物。  |  Prince, CW., et al. 1991. Biochem Biophys Res Commun. 177: 1205-10. PMID: 1676261
  2. 早期 T 淋巴细胞活化 1（Eta-1）蛋白与小鼠巨噬细胞体外特异性相互作用的定义及其对体内巨噬细胞的影响。  |  Singh, RP., et al. 1990. J Exp Med. 171: 1931-42. PMID: 2351930
  3. 补骨脂素的性质和意义。  |  Butler, WT. 1989. Connect Tissue Res. 23: 123-36. PMID: 2698313
  4. 正常大鼠肾细胞会分泌磷酸化和非磷酸化两种形式的骨化蛋白，它们具有不同的生理特性。  |  Nemir, M., et al. 1989. J Biol Chem. 264: 18202-8. PMID: 2808373
  5. 补骨脂素的结构域和功能域。  |  Butler, WT. 1995. Ann N Y Acad Sci. 760: 6-11. PMID: 7785926
  6. Osteopontin：一种具有多种功能的蛋白质。  |  Denhardt, DT. and Guo, X. 1993. FASEB J. 7: 1475-82. PMID: 8262332
  7. 骨, 牙齿和骨结合植入物中矿化组织界面上的骨素：超微结构分布及其对矿化组织形成, 更替和修复的影响。  |  McKee, MD. and Nanci, A. 1996. Microsc Res Tech. 33: 141-64. PMID: 8845514
  8. Eta-1/osteopontin 的免疫学。  |  Weber, GF. and Cantor, H. 1996. Cytokine Growth Factor Rev. 7: 241-8. PMID: 8971479

订购信息

产品名称产品编号规格价格数量收藏夹

osteopontin/OPN/SPP1 抗体 (AKm2A1)

sc-21742
200 µg/ml
RMB2377.00

osteopontin/OPN/SPP1 (AKm2A1): m-IgG Fc BP-HRP 套装

sc-526606
200 µg Ab; 10 µg BP
RMB2662.00

osteopontin/OPN/SPP1 (AKm2A1): m-IgG1 BP-HRP 套装

sc-531979
200 µg Ab; 20 µg BP
RMB2662.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) AC

sc-21742 AC
500 µg/ml, 25% agarose
RMB3129.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) HRP

sc-21742 HRP
200 µg/ml
RMB2377.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) FITC

sc-21742 FITC
200 µg/ml
RMB2482.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) PE

sc-21742 PE
200 µg/ml
RMB2580.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) Alexa Fluor® 488

sc-21742 AF488
200 µg/ml
RMB2685.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) Alexa Fluor® 546

sc-21742 AF546
200 µg/ml
RMB2685.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) Alexa Fluor® 594

sc-21742 AF594
200 µg/ml
RMB2685.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) Alexa Fluor® 647

sc-21742 AF647
200 µg/ml
RMB2685.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) Alexa Fluor® 680

sc-21742 AF680
200 µg/ml
RMB2685.00

osteopontin/OPN/SPP1 抗体 (AKm2A1) Alexa Fluor® 790

sc-21742 AF790
200 µg/ml
RMB2685.00

Do you offer a carrier protein and azide free version of this antibody?

Asked by: Hanno
Thank you for contacting Santa Cruz Biotechnology. For carrier protein and azide free products please contact our special orders department. Please email [email protected] for a price quote and ship date.
Answered by: BlakeJ
Date published: 2025-04-30

Olá! Qual espectro de excitação e emissão do anticorpo osteopontin/OPN/SPP1 Anticorpo (AKm2A1) Alexa Fluor® 594? What is the excitation and emission spectrum of the osteopontin/OPN/SPP1 Antibody (AKm2A1) Alexa Fluor® 594?

Asked by: Diego
Obrigado pela sua pergunta. O coeficiente de excitação é aproximadamente 91.000 e o máximo de emissão é 617 nm.
Answered by: BlakeJ
Date published: 2025-01-16

I want to use this product for immunofluorescence as I have it in the lab. Can you tell me what the dilution should be?

Asked by: Udipt
Thank you for your question. We list dilution recommendations in the antibody's datasheet https://datasheets.scbt.com/sc-21742.pdf: OPN (AKm2A1) is recommended for detection of OPN of mouse, rat and human origin by immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500).
Answered by: Tech Support Europe
Date published: 2024-10-24

sc-21742抗体可以做流式检测吗

Asked by: 12345
感谢您的提问。sc-21742不推荐做流式检测。 Thank you for your question. sc-21742 is not recommended for FCM detection.
Answered by: Technical Service SH
Date published: 2024-01-29

primary mouse monoclonal antibody to detect mouse protein-that will generate much background after anti-mouse 2nd antibody. Do you have specail prtotocol to avoid the effect? I need to do IHC with ABC method for mouse sample. Thank you! Kai

Asked by: Kal69
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2021-12-15

Hello, Does this antibody recognize the N-terminus celaved fragment of OPN, the C-terminus fragment or both?

Asked by: Chris G
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-10-11

Can I use this antibody for ip injection to neutralize the OPN in mice for long-term study?

Asked by: Yan T
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

How much would it be if I want to order 25 mg, 50 mg and 100 mg of this antibody for animal treatment (long-term)?

Asked by: Yan T
Our antibodies cannot be used for diagnostic or therapeutic purposes.
Answered by: Tech Sales
Date published: 2021-06-25
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Rated 5 out of 5 by from 很好用 借了别人的Santa抗体使用 效果不错很好用 借了别人的Santa抗体使用 效果不错 物美价廉 品质保证 我也来购买啦 出来的条带很干净。
Date published: 2024-08-18
Rated 5 out of 5 by from 很好用效价非常高，非常好用，可以反复回收使用，特别是检测转染的质粒表达，条带非常单一,非常开心，以后还会多多购买
Date published: 2022-11-24
Rated 5 out of 5 by from SK030204V100This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2022-01-05
Rated 5 out of 5 by from 功能很出色我一个月前购买了该抗体，用得很好，老板很满意，还要多写几个字，其实我也不知道写啥好。为了多写一些。 还少啊
Date published: 2022-01-05
Rated 5 out of 5 by from Very good antibodyWorks as we expected. Tested by immunofluorescence. Great
Date published: 2018-12-18
Rated 5 out of 5 by from Works well for IHCThis antibody was generously given to me by the trials program. I found that it works well for IHC at a dilution of 1:50 to 1:250 in whole mount of retinal tissue. It works better with PFA than Methanol fixation.
Date published: 2018-03-14
Rated 5 out of 5 by from 抗体很好用，条带特别单一。申请了试用装，抗体特别好用，特异性很好。从开始用到现在已经有三个月的时间，一直用的是开始配的抗体，用了五六次，抗体效价很高。
Date published: 2018-02-07
Rated 5 out of 5 by from good anti-bodyThis product is used in broiler pulmonary artery smooth muscle cells, the effect is good, 1: 50 dilution
Date published: 2018-01-10
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osteopontin/OPN/SPP1 抗体 (AKm2A1) is rated 4.5 out of 5 by 28.
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