Date published: 2025-9-7
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OPG/Osteoprotegerin Antibody (E-10) - Western Blotting - Image 361683
OPG 抗体 (E-10): sc-390518. HeLa (A), Neuro-2A (B) 和 C6 (C) 全细胞裂解液中 OPG 表达的 Western 印迹分析.
OPG 抗体 (E-10): sc-390518. HeLa (A), Neuro-2A (B) 和 C6 (C) 全细胞裂解液中 OPG 表达的 Western 印迹分析.

OPG/Osteoprotegerin 抗体 (E-10): sc-390518

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说明书
  • OPG/Osteoprotegerin 抗体 E-10 是小鼠单克隆 IgG1 κ，OPG/Osteoprotegerin抗体, 在32篇文献中引用，规格为200 µg/ml
  • 免疫human物种的OPG的氨基酸153-401
  • OPG/Osteoprotegerin 抗体 (E-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 OPG
  • 抗OPG/Osteoprotegerin抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-390518 X, 200 µg/0.1 ml)
  • 关于如何获取OPG/Osteoprotegerin (E-10): sc-390518的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是OPG/Osteoprotegerin Antibody (E-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与OPG/Osteoprotegerin Antibody (E-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

OPG抗体（E-10）是一种IgG1κ小鼠单克隆OPG抗体（也称为TNFRSF11B抗体或骨保护素抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的OPG蛋白。OPG抗体（E-10）既可作为非偶联抗OPG抗体形式提供，也可作为多种偶联形式的抗OPG抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。骨形态发生和重塑涉及成骨细胞形成骨骼和破骨细胞吸收骨骼。已知细胞因子骨保护素（OPG），也称为破骨细胞生成抑制因子（OCIF），可抑制破骨细胞的形成。作为一种分泌型糖蛋白，OPG是增加骨密度和体积的TNF受体家族成员。OPG被认为通过破坏成骨基质细胞与破骨细胞前体之间的细胞间信号传导来抑制破骨细胞生成。已知OPG与含有死亡结构域的蛋白TRAIL结合，并抑制Jurkat细胞中的TRAIL凋亡。OPG还与破骨细胞分化因子（ODF）结合，后者也称为TRANCE/RANKL，是一种属于TNF配体家族的膜结合蛋白。TNFα和TNFβ均可上调OPG表达，而骨吸收剂前列腺素E2则下调OPG。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

OPG/Osteoprotegerin 抗体 (E-10) 参考文献:

  1. RANKL-OPG和RAGE在血管钙化和糖尿病中的调节：新的治疗靶点。  |  Ndip, A., et al. 2014. Diabetologia. 57: 2251-60. PMID: 25112376
  2. RANKL/OPG平衡对减少植入物并发症的影响。  |  Kapasa, ER., et al. 2017. J Funct Biomater. 8: PMID: 28937598
  3. 快速上颌骨扩展中的RANK/RANKL/OPG表达。  |  Arnez, MFM., et al. 2017. Braz Dent J. 28: 296-300. PMID: 29297549
  4. RANKL-RANK-OPG对T细胞相关组织和T细胞活化的调节。  |  Walsh, MC. and Choi, Y. 2021. J Bone Miner Metab. 39: 54-63. PMID: 33438173
  5. 使用高频超声、血清RANKL和OPG检测筛查早期类风湿性关节炎。  |  Mo, H., et al. 2024. Clin Rheumatol. 43: 159-166. PMID: 38051416
  6. 骨蛋白gerin：一种参与调节骨密度的新型分泌蛋白。  |  Simonet, WS., et al. 1997. Cell. 89: 309-19. PMID: 9108485
  7. 破骨细胞生成抑制因子（OCIF）和骨保护蛋白（OPG）的特性：OPG/OCIF 在体外抑制破骨细胞生成的机制。  |  Yasuda, H., et al. 1998. Endocrinology. 139: 1329-37. PMID: 9492069
  8. 破骨细胞分化因子是骨保护蛋白/破骨细胞生成抑制因子的配体，与 TRANCE/RANKL 相同。  |  Yasuda, H., et al. 1998. Proc Natl Acad Sci U S A. 95: 3597-602. PMID: 9520411
  9. 骨蛋白激酶是细胞毒性配体 TRAIL 的受体。  |  Emery, JG., et al. 1998. J Biol Chem. 273: 14363-7. PMID: 9603945
  10. 前列腺素 E2 在人骨髓基质细胞中对骨保护素 mRNA 水平的调控。  |  Brändström, H., et al. 1998. Biochem Biophys Res Commun. 247: 338-41. PMID: 9642127
  11. 骨骼重塑  |  Hill, PA. 1998. Br J Orthod. 25: 101-7. PMID: 9668992
  12. 肿瘤坏死因子-α和-β能上调人骨肉瘤 MG-63 细胞中的骨保护素 mRNA 水平。  |  Brändström, H., et al. 1998. Biochem Biophys Res Commun. 248: 454-7. PMID: 9703945

订购信息

产品名称产品编号规格价格数量收藏夹

OPG/Osteoprotegerin 抗体 (E-10)

sc-390518
200 µg/ml
RMB2377.00

OPG/Osteoprotegerin (E-10): m-IgG Fc BP-HRP 套装

sc-530178
200 µg Ab; 10 µg BP
RMB2662.00

OPG/Osteoprotegerin (E-10): m-IgGκ BP-HRP 套装

sc-523568
200 µg Ab, 40 µg BP
RMB2662.00

OPG/Osteoprotegerin (E-10): m-IgG1 BP-HRP 套装

sc-543908
200 µg Ab; 20 µg BP
RMB2662.00

OPG/Osteoprotegerin 抗体 (E-10) X

sc-390518 X
200 µg/0.1 ml
RMB2377.00

OPG/Osteoprotegerin 抗体 (E-10) AC

sc-390518 AC
500 µg/ml, 25% agarose
RMB3129.00

OPG/Osteoprotegerin 抗体 (E-10) HRP

sc-390518 HRP
200 µg/ml
RMB2377.00

OPG/Osteoprotegerin 抗体 (E-10) FITC

sc-390518 FITC
200 µg/ml
RMB2482.00

OPG/Osteoprotegerin 抗体 (E-10) PE

sc-390518 PE
200 µg/ml
RMB2580.00

OPG/Osteoprotegerin 抗体 (E-10) Alexa Fluor® 488

sc-390518 AF488
200 µg/ml
RMB2685.00

OPG/Osteoprotegerin 抗体 (E-10) Alexa Fluor® 546

sc-390518 AF546
200 µg/ml
RMB2685.00

OPG/Osteoprotegerin 抗体 (E-10) Alexa Fluor® 594

sc-390518 AF594
200 µg/ml
RMB2685.00

OPG/Osteoprotegerin 抗体 (E-10) Alexa Fluor® 647

sc-390518 AF647
200 µg/ml
RMB2685.00

OPG/Osteoprotegerin 抗体 (E-10) Alexa Fluor® 680

sc-390518 AF680
200 µg/ml
RMB2685.00

OPG/Osteoprotegerin 抗体 (E-10) Alexa Fluor® 790

sc-390518 AF790
200 µg/ml
RMB2685.00

お世話になります。OPG/Osteoprotegerin (E-10): sc-390518のサンプルを頂きたいのですが、可能でしょうか。

Asked by: Ayumu
Thank you very much for your question. Please ask your Japanese dealer to apply for sample products. Website: www.cosmobio.co.jp ， www.iwai-chem.co.jp or www.nacalai.co.jp/ss/Contact/ 質問頂き、誠にありがとうございます。サンプル品の申請は日本のディーラーにお尋ねお願いします。ウェブサイト: www.cosmobio.co.jp ， www.iwai-chem.co.jp 又はwww.nacalai.co.jp/ss/Contact/
Answered by: Jiajun Z
Date published: 2024-10-29

Hej! Jag skall använda antikropp sc-390518 för IHC staining och letar efter en bra secundär antikropp. Vad rekommenderar ni? Andra frågan gäller serum, vad rekommenderas? Tacksam för svar! mvh AN

Asked by: Annb
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

I am seeing 2 bands: at 50 KDa and a stronger one at 65 KDa for fibroblast lysate in WB (USING OPG (E-10) ANTIBODY), Which one is the real OPG, BECAUSE the two bands have different level of expression.

Asked by: hudhud
Thank you for your question. We want you to be successful in your research. Please reach out to your local distributor or contact Technical Service directly. We are happy to help you determine the accuracy of the bands you're seeing and help optimize and troubleshoot to make sure you have success with the antibody.
Answered by: Tech Service
Date published: 2019-10-14

Can sc-422063-HDR be used in IHC with mouse tissue? Is there any successful picture to show us ? Thank you!

Asked by: sumer
Thank you for your question. sc-422063-HDR consists of a pool of 2-3 homologous directed repair (HDR) plasmids which are only used when co-transfected with CRISPR/Cas9 KO Plasmids for gene knockout purpose. For IHC application, we recommend using a directly conjugated antibody. OPG Antibody (E-10): sc-390518 is available conjugated to HRP (sc-390518 HRP) for WB, IHC and ELISA. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website. Best of luck with your research!
Answered by: Technical Support 14
Date published: 2017-07-10

Can OPG (E-10): sc-390518 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, OPG (E-10): sc-390518 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Which OPG antibody is recommended for histopathology? Is a sample available of the recommended one as is is on your homepage at the goat or rabbit ab against OPG? Thank you, Jolan Harsfalvi, MSc, senior adviser

Asked by: Jolan
Thank you for your question. OPG Antibody (E-10): sc-390518 is a mouse monoclonal antibody which is currently recommended for use in WB, IP, IF (immunoperoxidase, or immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone) and ELISA. This antibody has not yet been tested for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections. Please contact our Technical Service Department to request a free 10 µg sample if you would like to test the antibody in your intended research application. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-12
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Rated 5 out of 5 by from Excellent western blot !Got the sample one month ago. Excellent western blot !
Date published: 2017-08-25
Rated 5 out of 5 by from clear bandWB experiments are very successful, there can always be a clear band, WB effect is very good.
Date published: 2017-05-31
Rated 5 out of 5 by from Clean bands with backgroundWe used OPG antibody Western blot analysis of expression in rat spleen tissue extract,the effect is quite good, the quality is stable.
Date published: 2017-04-16
Rated 5 out of 5 by from recommended ！！！Strong and specific band in western blot for human recombinant OPG fusion protein, Do not hesitate, strongly recommended!
Date published: 2017-02-07
Rated 5 out of 5 by from Nice detection of OPG expressionWe used this antibody for a western blot and got a very nice band at the correct molecular weight in HeLa cells
Date published: 2017-01-27
Rated 5 out of 5 by from 功能很出色我在两周前申请了sc-390518的抗体，做了免疫组化的实验，染色结果很好，抗体特异性好，很少背景着色。
Date published: 2016-09-22
Rated 5 out of 5 by from Great Western Blot data of OPG expression in nonGreat Western Blot data of OPG expression in non-transfected and human OPG transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-05-02
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OPG/Osteoprotegerin抗体 (E-10) is rated 5.0 out of 5 by 7.
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