Date published: 2025-9-12
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Omni-probe Antibody (D-8) - Western Blotting - Image 5607
全能探针抗体 (D-8): sc-7270. 全能标签融合蛋白的 Western 印迹分析, Syk (257-352): sc-4063 WB (A) 和 ATF-1(39-271): sc-4006 WB (B).
Omni-probe Antibody (D-8) - Western Blotting - Image 5607
全能探针抗体 (D-8): sc-7270. 全能标签融合蛋白的 Western 印迹分析, Syk (257-352): sc-4063 WB (A) 和 ATF-1(39-271): sc-4006 WB (B).
全能探针抗体 (D-8): sc-7270. 全能标签融合蛋白的 Western 印迹分析, Syk (257-352): sc-4063 WB (A) 和 ATF-1(39-271): sc-4006 WB (B).

Omni-probe 抗体 (D-8): sc-7270

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说明书
  • Omni-probe 抗体 D-8 是小鼠单克隆 IgG2a κ，Omni-probe抗体, 在59篇文献中引用，规格为200 µg/ml
  • 特定于（His）6和多连接子序列内8-30氨基酸之间的表位图谱
  • Omni-probe 抗体 (D-8) 推荐用于 WB 和 IP，检测 fusion proteins encoded by the prokaryotic pRSET A, B, C and pTrc His A, B, C; baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors
  • 抗Omni-probe抗体（D-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Omni-probe (D-8): sc-7270的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是Omni-probe Antibody (D-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Omni-probe Antibody (D-8) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

Omni-probe抗体（D-8）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测Omni-probe。Omni-probe (D-8)抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。Omni-probe在鉴定和纯化混合融合蛋白方面发挥着至关重要的作用，这些蛋白通常是通过质粒载体产生的，该载体在各种宿主系统（如大肠杆菌、昆虫和哺乳动物细胞）中表达真核基因的编码区域。这些载体通常编码结合原核和真核元素的混合蛋白，从而能够高效地生产和纯化所需蛋白。这些融合蛋白中存在聚组氨酸序列，因此可以使用带镍的琼脂糖树脂快速纯化，这使得Omni-probe (D-8)单克隆抗体成为研究表达融合蛋白的科研人员的必备工具。Omni-probe (D-8)抗体通过靶向聚组氨酸标签和cDNA插入物之间的特定载体序列，可以方便地识别不同宿主系统中的表达蛋白，从而简化研究过程并提高实验结果。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Omni-probe 抗体 (D-8) 参考文献:

  1. 用于多调控多基因代谢工程的多功能大环内酯响应哺乳动物表达载体。  |  Weber, W., et al. 2002. Biotechnol Bioeng. 80: 691-705. PMID: 12378611
  2. 将外来蛋白质纳入病毒闭锁体的杆状病毒表达载体。  |  Je, YH., et al. 2003. Biotechniques. 34: 81-7. PMID: 12545544
  3. 利用杆状病毒表达载体在昆虫细胞中组装功能 GABAA 受体。  |  Atkinson, AE., et al. 1992. Neuroreport. 3: 597-600. PMID: 1330062
  4. 利用表达增强序列元件（EASE）改进哺乳动物双组分表达载体。  |  Aldrich, TL., et al. 1998. Cytotechnology. 28: 9-17. PMID: 19003402
  5. 制备含有特定位点板层 DNA 病变的哺乳动物表达载体。  |  Ang, WH., et al. 2009. Bioconjug Chem. 20: 1058-63. PMID: 19351185
  6. 昆虫细胞培养中使用杆状病毒表达载体的共表达与共感染：优点与缺点。  |  Sokolenko, S., et al. 2012. Biotechnol Adv. 30: 766-81. PMID: 22297133
  7. 哺乳动物表达载体，通过顺式共同表达哺乳动物密码子优化的 BirA 生物素连接酶，实现代谢生物素化串联亲和标记。  |  Ioannou, M., et al. 2018. BMC Res Notes. 11: 390. PMID: 29898783
  8. 使用杆状病毒表达载体的最新进展。  |  Possee, RD., et al. 2020. Curr Issues Mol Biol. 34: 215-230. PMID: 31167962
  9. 构建网关兼容的杆状病毒表达载体，用于高通量蛋白质表达和体内微晶筛选。  |  Tang, Y., et al. 2020. Sci Rep. 10: 13323. PMID: 32770037
  10. 由 GAGA 转录因子结合介导的热休克启动子的 ATP 依赖性核小体破坏。  |  Tsukiyama, T., et al. 1994. Nature. 367: 525-32. PMID: 8107823
  11. p42 微管相关蛋白 2 激酶和蛋白激酶 C 对高分子量形式的磷脂酶 A2 进行磷酸化和激活。  |  Nemenoff, RA., et al. 1993. J Biol Chem. 268: 1960-4. PMID: 8380583
  12. 中性粒细胞磷脂酶 D 是由膜缔合作用的 Rho 家族小分子量 GTP 结合蛋白激活的。  |  Bowman, EP., et al. 1993. J Biol Chem. 268: 21509-12. PMID: 8408000

订购信息

产品名称产品编号规格价格数量收藏夹

Omni-probe 抗体 (D-8)

sc-7270
200 µg/ml
RMB2377.00

Omni-probe (D-8): m-IgG Fc BP-HRP 套装

sc-525432
200 µg Ab; 10 µg BP
RMB2662.00

Omni-probe (D-8): m-IgG2a BP-HRP 套装

sc-546253
200 µg Ab; 10 µg BP
RMB2662.00

Omni-probe 抗体 (D-8) AC

sc-7270 AC
500 µg/ml, 25% agarose
RMB3129.00

Omni-probe 抗体 (D-8) HRP

sc-7270 HRP
200 µg/ml
RMB2377.00

Omni-probe 抗体 (D-8) FITC

sc-7270 FITC
200 µg/ml
RMB2482.00

Omni-probe 抗体 (D-8) PE

sc-7270 PE
200 µg/ml
RMB2580.00

Omni-probe 抗体 (D-8) Alexa Fluor® 488

sc-7270 AF488
200 µg/ml
RMB2685.00

Omni-probe 抗体 (D-8) Alexa Fluor® 546

sc-7270 AF546
200 µg/ml
RMB2685.00

Omni-probe 抗体 (D-8) Alexa Fluor® 594

sc-7270 AF594
200 µg/ml
RMB2685.00

Omni-probe 抗体 (D-8) Alexa Fluor® 647

sc-7270 AF647
200 µg/ml
RMB2685.00

Omni-probe 抗体 (D-8) Alexa Fluor® 680

sc-7270 AF680
200 µg/ml
RMB2685.00

Omni-probe 抗体 (D-8) Alexa Fluor® 790

sc-7270 AF790
200 µg/ml
RMB2685.00

Omni-probe (D-8) 中和勝肽

sc-7270 P
100 µg/0.5 ml
RMB511.00

What is your recommended western blotting secondary antibody for use with Omni-probe (D-8): sc-7270?

Asked by: jenniferc
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-02-04
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Rated 5 out of 5 by from Excellent choice for Omni-probe detectionThis mouse monoclonal (D-8) clone is an excellent choice for detection of Omni-probe labeled proteins. It has been featured in over 30 journal articles!
Date published: 2017-01-19
Rated 5 out of 5 by from Good Western bands detected with OmniGood Western bands detected with Omni-tag fusion proteins -SCBT QC
Date published: 2015-07-05
Rated 4 out of 5 by from Nice publishable data on Western blot onNice publishable data on Western blot on canine MDCK, D-17 and Cf2Th cells . -SCBT Publication Review
Date published: 2015-06-30
Rated 5 out of 5 by from Published data for IF in human and mousePublished data for IF in human and mouse HEK293 and MCF-7 cells. -SCBT Publication Review
Date published: 2015-06-30
Rated 5 out of 5 by from Published IHC(P) data for use in mouse C4Published IHC(P) data for use in mouse C4-HD and C4-HI tumors. -SCBT Publication Review
Date published: 2015-06-19
Rated 5 out of 5 by from Published data for IP and WB in canine MKN28Published data for IP and WB in canine MKN28, AGS, COS-7, and MDCK (MDCK II) cells. -SCBT Publication Review
Date published: 2015-04-17
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with human and mouse BP-A31 and COS-7 cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 4 out of 5 by from WB: Worked well in mouse NIH 3T3 cellsWB: Worked well in mouse NIH 3T3 cells. -SCBT Publication Review
Date published: 2015-03-26
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Omni-probe 抗体 (D-8) is rated 4.6 out of 5 by 10.
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