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OGG1/2 Antibody (G-5) - Western Blotting - Image 356643
OGG1/2 抗体 (G-5): sc-376935. HeLa (A) 和 Jurkat (B) 全细胞裂解液中 OGG1/2 表达的 Western 印迹分析.
OGG1/2 抗体 (G-5): sc-376935. HeLa (A) 和 Jurkat (B) 全细胞裂解液中 OGG1/2 表达的 Western 印迹分析.

OGG1/2 抗体 (G-5): sc-376935

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说明书
  • OGG1/2 抗体 G-5 是小鼠单克隆 IgG1 κ，OGG1/2抗体, 在19篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的OGG1/2的N-terminus附近的氨基酸13-49之间
  • OGG1/2 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 OGG1 and OGG2
  • 抗OGG1/2抗体（G-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取OGG1/2 (G-5): sc-376935的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是OGG1/2 Antibody (G-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与OGG1/2 Antibody (G-5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加OGG抗体，包括OGG1和OGG2

OGG1/2抗体（G-5）是一种IgG1 κ小鼠单克隆OGG1/2抗体（也称为8-氧鸟嘌呤DNA糖基化酶抗体、MUTM抗体、OGH1抗体、8-羟基鸟嘌呤DNA糖基化酶抗体、N-糖基化酶/DNA裂解酶抗体、HOGG1抗体、HMMH抗体、DNA-无嘌呤裂解酶抗体或DNA-无嘧啶裂解酶抗体），它能够通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的OGG1/2蛋白。OGG1/2抗体（G-5）既有非偶联形式的抗OGG1/2抗体，也有多种偶联形式的抗OGG1/2抗体，包括琼脂糖、HRP、PE、FITC以及多种Alexa Fluor®偶联物。8-氧鸟嘌呤（8-oxoG）是鸟嘌呤的氧化形式，在正常衰老过程中由活性氧在DNA和核苷酸池中产生。8-oxoG的积累增加了A:T到C:G或G:C到T:A颠换突变的发生，因为8-oxoG与腺嘌呤以及胞嘧啶形成了稳定的碱基对。OGG1（代表8-oxoG DNA糖基化酶），也称为MMH，是一种DNA修复酶，可以纠正这些突变。OGG1基因的失活会导致突变表型，其特征在于G:C到T:A颠换的增加。OGG1基因通过单个信使RNA的选择性剪接编码8种同工型（OGG1A-C、OGG2A-E）。OGG1A剪接变体是最普遍的形式，定位于细胞核，而OGG2A剪接变体则靶向线粒体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

OGG1/2 抗体 (G-5) 参考文献:

  1. 由交替剪接的 OGG1 mRNA 编码的两种主要形式的人类 8-氧代鸟嘌呤 DNA 糖基化酶的表达和细胞内定位差异。  |  Nishioka, K., et al. 1999. Mol Biol Cell. 10: 1637-52. PMID: 10233168
  2. Mmh/Ogg1 基因失活会导致小鼠体内 8-羟基鸟嘌呤的积累。  |  Minowa, O., et al. 2000. Proc Natl Acad Sci U S A. 97: 4156-61. PMID: 10725358
  3. 人类 OGG1 基因：结构, 功能及其在致癌过程中的影响。  |  Boiteux, S. and Radicella, JP. 2000. Arch Biochem Biophys. 377: 1-8. PMID: 10775435
  4. 人类2型糖尿病中胰岛DNA修复酶8-氧代鸟嘌呤DNA糖基化酶（Ogg1）的表达。  |  Tyrberg, B., et al. 2002. BMC Endocr Disord. 2: 2. PMID: 12003641
  5. 8-羟基鸟嘌呤是 DNA 氧化损伤的一种常见形式，会导致 G----T 和 A----C 的置换。  |  Cheng, KC., et al. 1992. J Biol Chem. 267: 166-72. PMID: 1730583
  6. OGG1基因中的Ser326Cys多态性与2型DM的关系。  |  Daimon, M., et al. 2009. Biochem Biophys Res Commun. 386: 26-9. PMID: 19486888
  7. DNA 合成过程中特定碱基的插入经过了氧化损伤碱基 8-oxodG。  |  Shibutani, S., et al. 1991. Nature. 349: 431-4. PMID: 1992344
  8. 线粒体靶向Ogg1和aconitase-2可防止氧化剂诱导的肺泡上皮细胞线粒体DNA损伤。  |  Kim, SJ., et al. 2014. J Biol Chem. 289: 6165-76. PMID: 24429287
  9. 长非编码RNA NONHSAT143692.2通过调节miR‑4728‑5p/OGG1轴参与晶状体氧化DNA损伤修复。  |  Zhou, T., et al. 2020. Int J Mol Med. 46: 1838-1848. PMID: 33000245
  10. MRC-5人肺成纤维细胞通过OGG1/2依赖性修复机制减轻Fe-N共掺二氧化钛纳米粒子的基因毒性效应  |  Miu, BA., et al. 2023. Int J Mol Sci. 24: PMID: 37047374
  11. 氧化剂, 抗氧化剂和衰老退化性疾病。  |  Ames, BN., et al. 1993. Proc Natl Acad Sci U S A. 90: 7915-22. PMID: 8367443
  12. 与年龄相关的人类心脏线粒体 DNA 损伤。  |  Hayakawa, M., et al. 1993. Mol Cell Biochem. 119: 95-103. PMID: 8455592

订购信息

产品名称产品编号规格价格数量收藏夹

OGG1/2 抗体 (G-5)

sc-376935
200 µg/ml
RMB2377.00

OGG1/2 (G-5): m-IgG Fc BP-HRP 套装

sc-529951
200 µg Ab; 10 µg BP
RMB2662.00

OGG1/2 (G-5): m-IgGκ BP-HRP 套装

sc-523137
200 µg Ab, 40 µg BP
RMB2662.00

OGG1/2 (G-5): m-IgG1 BP-HRP 套装

sc-543781
200 µg Ab; 20 µg BP
RMB2662.00

OGG1/2 抗体 (G-5) AC

sc-376935 AC
500 µg/ml, 25% agarose
RMB3129.00

OGG1/2 抗体 (G-5) HRP

sc-376935 HRP
200 µg/ml
RMB2377.00

OGG1/2 抗体 (G-5) FITC

sc-376935 FITC
200 µg/ml
RMB2482.00

OGG1/2 抗体 (G-5) PE

sc-376935 PE
200 µg/ml
RMB2580.00

OGG1/2 抗体 (G-5) Alexa Fluor® 488

sc-376935 AF488
200 µg/ml
RMB2685.00

OGG1/2 抗体 (G-5) Alexa Fluor® 546

sc-376935 AF546
200 µg/ml
RMB2685.00

OGG1/2 抗体 (G-5) Alexa Fluor® 594

sc-376935 AF594
200 µg/ml
RMB2685.00

OGG1/2 抗体 (G-5) Alexa Fluor® 647

sc-376935 AF647
200 µg/ml
RMB2685.00

OGG1/2 抗体 (G-5) Alexa Fluor® 680

sc-376935 AF680
200 µg/ml
RMB2685.00

OGG1/2 抗体 (G-5) Alexa Fluor® 790

sc-376935 AF790
200 µg/ml
RMB2685.00

OGG1/2 (G-5) 中和勝肽

sc-376935 P
100 µg/0.5 ml
RMB511.00

Can this antibody be used for immunohistochemical paraffin sections of tissue? I am looking forward to your reply. Thank you!

Asked by: July Wang
The HRP-conjugate or fluorchrome-conjugated versions of this product would be appropriate for immunohistochemistry on paraffin-embedded tissue sections. We recommend including antigen retrieval steps for any protocol that includes fixation with paraformaldehyde or formalin. Please refer to Section C in the following link. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Help
Date published: 2018-01-23

Can the blocking peptide sc-376935 P be used as a positive control in Western Blot?

Asked by: AbPolly
Thank you for your question. The blocking peptide is a short synthetic peptide sequence and is not appropriate to be used as a positive control in Western Blot. It is intended as a negative control by preadsorbing the antibody prior to application.
Answered by: Technical Support 1
Date published: 2017-01-16
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Rated 4 out of 5 by from Good band but at a different MWThe ab gives a great band in WB but not at 43 kDa but much lower at around 30 kDa.
Date published: 2021-07-07
Rated 4 out of 5 by from Excellent for IP-WBMitochondrial OGG1/2 protein from neuronal cells was detected by co-immunoprecipitation with proteinL-conjugate
Date published: 2021-03-24
Rated 5 out of 5 by from Sensitive in western blotI used this antibody in co-immunoprecipitation to detect OGG1 in A549 cell lysates. Excellent features.
Date published: 2017-12-01
Rated 5 out of 5 by from Great antibodyI really recommend this antibody for WB detection. I got very nice and strong bands for MCF7 cells lines.
Date published: 2017-11-29
Rated 5 out of 5 by from Excellent for western blotSensitive detection of OGG1 in cell lysate (THP1) even at low protein concentration
Date published: 2017-10-09
Rated 5 out of 5 by from ExcellentI really recommend this antibody for WB, I got very nice and strong band with little to no background!
Date published: 2017-03-30
Rated 5 out of 5 by from Great AntibodyOGG1/2 (G-5) gives a beautiful band in Hela whole cell lyate.
Date published: 2016-12-21
Rated 5 out of 5 by from Our WB data looked goodOur WB data looked good.
Date published: 2015-04-08
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OGG1/2 抗体 (G-5) is rated 4.8 out of 5 by 9.
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