Date published: 2025-9-11
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OctA-Probe Antibody (H-5) - Western Blotting - Image 72807
OctA-Probe 抗体 (H-5): sc-166355. OctA (FLAG®) 标记融合蛋白的 Western 印迹分析.
OctA-Probe Antibody (H-5) - Western Blotting - Image 72807
OctA-Probe 抗体 (H-5): sc-166355. OctA (FLAG®) 标记融合蛋白的 Western 印迹分析.
OctA-Probe Antibody (H-5) - Western Blotting - Image 399489
OctA-Probe 抗体 (H-5) HRP: sc-166355 HRP. 直接 Western 印迹分析 OctA (FLAG®) 标记的融合蛋白.
OctA-Probe 抗体 (H-5): sc-166355. OctA (FLAG®) 标记融合蛋白的 Western 印迹分析.

OctA-Probe 抗体 (H-5): sc-166355

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寫評論提問

说明书
  • OctA-Probe 抗体 H-5 是小鼠单克隆 IgG1 κ，OctA-Probe抗体, 在111篇文献中引用，规格为200 µg/ml
  • 针对OctA（FLAG®）标记的蛋白
  • OctA-Probe 抗体 (H-5) 推荐用于 WB, IP, IF 和 ELISA，检测OctA 和FLAG tagged proteins 来源的 OctA (FLAG®)-tagged fusion proteins
  • 抗OctA-Probe抗体（H-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取OctA-Probe (H-5): sc-166355的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是OctA-Probe Antibody (H-5) for WB applications. 的首选辅助检测试剂，这些试剂现与OctA-Probe Antibody (H-5) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

OctA-Probe抗体（H-5）是一种IgG1 κ小鼠单克隆OctA-Probe抗体（也称为FLAG标签抗体、DYKDDDDK抗体），可通过WB、IP、IF和ELISA检测OctA和FLAG标记的蛋白质起源的OctA-Probe蛋白。OctA-Probe抗体（H-5）既以非偶联抗OctA-Probe抗体形式提供，也以多种偶联形式的抗OctA-Probe抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。质粒载体通常用于在细菌、昆虫和哺乳动物宿主中表达真核基因的编码区；这种表达载体经常用于编码由真核靶蛋白和专门设计的区域组成的杂交融合蛋白，以辅助靶蛋白的纯化和可视化。一个由八个氨基酸组成的小亲水性肽已被工程化到由各种原核和真核表达载体表达的蛋白质的N端。这种小肽已被证明在可视化和免疫亲和纯化表达的融合蛋白方面非常有用，并且由于肽部分的小尺寸和其亲水性质，表达的蛋白质经常保持其高水平的生物活性。此外，这八个氨基酸部分可以通过肠激酶切割去除。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

OctA-Probe 抗体 (H-5) 参考文献:

  1. Sprouty 与 Cbl 直接相互作用的证据  |  Wong, ES., et al. 2001. J Biol Chem. 276: 5866-75. PMID: 11053437
  2. α2整合素亚基细胞质结构域依赖的细胞迁移需要p38 MAPK。  |  Klekotka, PA., et al. 2001. J Biol Chem. 276: 9503-11. PMID: 11121402
  3. 由人类 AR 启动子控制的 CAG 重复扩增的转基因小鼠会出现多聚谷氨酰胺核内含物和神经元功能障碍，但不会导致神经元细胞死亡。  |  Adachi, H., et al. 2001. Hum Mol Genet. 10: 1039-48. PMID: 11331614
  4. 小鼠瞬态受体电位蛋白同源物 TRP1beta 的通道功能所需的结构域。  |  Engelke, M., et al. 2002. FEBS Lett. 523: 193-9. PMID: 12123831
  5. 纹状体神经元中不同信号通路对 ania-6 剪接变体的调控  |  Sgambato, V., et al. 2003. J Neurochem. 86: 153-64. PMID: 12807435
  6. 依赖于分化的脱丝体粘连蛋白desmoglein 1是调节角质形成细胞凋亡的新型caspase-3靶标。  |  Dusek, RL., et al. 2006. J Biol Chem. 281: 3614-24. PMID: 16286477
  7. 在大肠杆菌中表达化学合成的荷尔蒙体生长抑素基因。  |  Itakura, K., et al. 1977. Science. 198: 1056-63. PMID: 412251
  8. 在大肠杆菌中表达化学合成的人胰岛素基因。  |  Goeddel, DV., et al. 1979. Proc Natl Acad Sci U S A. 76: 106-10. PMID: 85300

订购信息

产品名称产品编号规格价格数量收藏夹

OctA-Probe 抗体 (H-5)

sc-166355
200 µg/ml
RMB2377.00

OctA-Probe (H-5): m-IgG Fc BP-HRP 套装

sc-527155
200 µg Ab; 10 µg BP
RMB2662.00

OctA-Probe (H-5): m-IgG1 BP-HRP 套装

sc-532528
200 µg Ab; 20 µg BP
RMB2662.00

OctA-Probe 抗体 (H-5) AC

sc-166355 AC
500 µg/ml, 25% agarose
RMB3129.00

OctA-Probe 抗体 (H-5) HRP

sc-166355 HRP
200 µg/ml
RMB2377.00

OctA-Probe 抗体 (H-5) FITC

sc-166355 FITC
200 µg/ml
RMB2482.00

OctA-Probe 抗体 (H-5) PE

sc-166355 PE
200 µg/ml
RMB2580.00

OctA-Probe 抗体 (H-5) Alexa Fluor® 488

sc-166355 AF488
200 µg/ml
RMB2685.00

OctA-Probe 抗体 (H-5) Alexa Fluor® 546

sc-166355 AF546
200 µg/ml
RMB2685.00

OctA-Probe 抗体 (H-5) Alexa Fluor® 594

sc-166355 AF594
200 µg/ml
RMB2685.00

OctA-Probe 抗体 (H-5) Alexa Fluor® 647

sc-166355 AF647
200 µg/ml
RMB2685.00

OctA-Probe 抗体 (H-5) Alexa Fluor® 680

sc-166355 AF680
200 µg/ml
RMB2685.00

OctA-Probe 抗体 (H-5) Alexa Fluor® 790

sc-166355 AF790
200 µg/ml
RMB2685.00

OctA-Probe (H-5) 中和勝肽

sc-166355 P
100 µg/0.5 ml
RMB511.00

Does the OctA-Probe Antibody (H-5): sc-166355 recognizes the 3x Flag: DYKDHDG-DYKDHDI-DYKDDDDK as it does for Flag: DYKDDDDK ? I could not find the actual amino acid sequence of the Octa (eight) peptide in Santa Cruz site. Thanks!

Asked by: Hect
Thank you for your question. The specific sequences used for our peptide antibodies, such as, sc-166355, is considered proprietary information. Please contact our Technical Service Department and we are happy to address any specific question you may have about the reactivity of this antibody.
Answered by: Dallas Tech Service
Date published: 2024-10-14

Has anyone used it for detection of FLAG-tagged proteins by flow cytometry? I can't find any sample data. I know it works ok for WB, but I would like to see that someone had it working for flow before I buy a PE conjugate...

Asked by: tamois
We are not yet aware of any product citations using this antibody for flow cytometry. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2020-05-15

Where does this protein（FLAG） express in paraffin embedded colon samples when do immune histochemistry？

Asked by: rujingai
Thank you for your question. That is a question we can't answer for you as it depends on the expression of the protein you have tagged with the Flag Tag (OctA probe). Please contact Technical Service if you have any further questions.
Answered by: Tech Service
Date published: 2019-05-10

I was wondering if we could get a sample of this antibody to test for on cell ELISA.

Asked by: DanielH
Thank you for your question. We are currently offering a free 10 µg sample of this antibody, OctA (DYKDDDDK)-Probe (H-5): sc-166355, if you want to test it before you place your order. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2021-08-08

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2017-01-06
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Rated 5 out of 5 by from Strong signal at 1:5,000Even at 1:5,000, the signal was too strong. It is necessary to say that I used overexpressed SOD1-flag system on HEK293T cells, loading less than 1µg of total protein. But it works well. It also works well for immunoprecipitation, I got pretty consistent readouts.
Date published: 2025-06-20
Rated 5 out of 5 by from Great ab for WBIt works very well for Western Blot. Working dilution 1:1000
Date published: 2020-10-07
Rated 5 out of 5 by from Perfect antibody to FLAG (DDK) peptideI used this clone to detect FLAG (DDK)-tagged protein in western blotting and immunocytostaining. This antibody was also used for immunoprecipitation. All application was done by this antibody. Well done.
Date published: 2019-08-28
Rated 4 out of 5 by from Worked fine.We used this antibody for WB for His-tagged proteins in bacteria, and it worked fine.
Date published: 2019-04-26
Rated 4 out of 5 by from Data is not as clear as the old GFP ab which wasData is not as clear as the old GFP ab which was discontinued in Santa cruz
Date published: 2018-11-04
Rated 5 out of 5 by from Excellent for western blotting!It detected Flag-tagged protein in transfected HEK 293T with very little background (1:1000 in 5% milk in TBS-0.1% Tween overnight at 4C).
Date published: 2018-04-09
Rated 3 out of 5 by from Nonspecific band at 75, also detects flag proteinFlag tagged protein detected (~48kDa), and non-specific band seen at 75kDa seen in 3 different human cell lines.
Date published: 2018-04-04
Rated 5 out of 5 by from Great!Great! It worked 1:5000 when I tested in my transfected cells
Date published: 2018-04-03
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OctA-Probe 抗体 (H-5) is rated 4.1 out of 5 by 39.
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