Date published: 2025-9-10

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OctA-Probe 抗体 (F-tag-01): sc-51590

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说明书
  • OctA-Probe抗体(F-tag-01)是小鼠单克隆IgG1 κ, 在19篇文献中引用,规格为100 µg/ml
  • 针对OctA(DYKDDDDK)的合成肽
  • 推荐用于WB, IP和IF检测
  • 看 OctA-Probe (H-5): sc-166355,可查看有标记的 OctA-Probe 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取OctA-Probe (F-tag-01): sc-51590免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是OctA-Probe Antibody (F-tag-01) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与OctA-Probe Antibody (F-tag-01) 打包提供(请参阅下面的订购信息)。

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関連項目

OctA-Probe抗体(F-tag-01)是一种IgG1 κ小鼠单克隆OctA-Probe抗体(也称为OctA-Probe抗体或FLAG标签抗体),可通过WB、IP和IF检测带有OctA(FLAG®)标签的融合蛋白。OctA-Probe抗体(F-tag-01)以非偶联抗OctA-Probe抗体形式提供。质粒载体用于在细菌、昆虫和哺乳动物宿主中表达真核基因的编码区,这是常见的用法;这种表达载体经常用于编码由真核靶蛋白和专门设计的区域组成的杂交融合蛋白,以辅助靶蛋白的纯化和可视化。一个由八个氨基酸组成的小亲水性肽已被工程化到由各种原核和真核表达载体表达的蛋白质的N端。这种小肽已被证明在可视化和免疫亲和纯化表达的融合蛋白方面非常有用,并且由于肽部分的小尺寸和其亲水性质,表达的蛋白质经常保持其高水平的生物活性。此外,这八个氨基酸部分可以通过肠激酶切割去除。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

OctA-Probe 抗体 (F-tag-01)

sc-51590
100 µg/ml
RMB2377.00

OctA-Probe (F-tag-01): m-IgG Fc BP-HRP 套装

sc-536849
100 µg Ab; 10 µg BP
RMB2662.00

OctA-Probe (F-tag-01): m-IgGκ BP-HRP 套装

sc-534016
100 µg Ab; 40 µg BP
RMB2662.00

OctA-Probe (F-tag-01): m-IgG1 BP-HRP 套装

sc-544856
100 µg Ab; 20 µg BP
RMB2662.00

At what molecular weight should the band be seen in Western Blot with OctA-Probe (F-tag-01): sc-51590 monoclonal antibody?

Asked by: jenni
Thank you for your question. The molecular weight expected varies depending on the fusion protein you are trying to detect, equal to the protein of interest plus the weight of the OctA tag that it is fused to.
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 3 out of 5 by from Poor signal in yeast lysateThis did not work as well as the commercial Ab I use from a different company against a FLAG-tagged protein in budding yeast lysate. A very faint band could be detected but was not as bright as what I see with the other company's Ab.
Date published: 2018-02-12
Rated 5 out of 5 by from Hight titer antibodyThis antibody reacts specific to OctA-Probe.We uset 1:800 dilution by loading cell lysates 60ug.
Date published: 2018-01-27
Rated 3 out of 5 by from Western BlotUsed trial in Western blot looking at transfected HeLa lysate, but found non-specific band a little bit above 75kDa.
Date published: 2017-10-19
Rated 5 out of 5 by from Good Probe AntibodyOctA-Probe (F-tag-01) works great for Western blot (WB) in detecting OctA tagged recombinant fusion protein.
Date published: 2017-01-14
Rated 5 out of 5 by from Get good resultsGet good results for WB and IP in 293T cells. And the price was acceptable.
Date published: 2017-01-10
Rated 5 out of 5 by from Published data for WB in Dictyostelium discoideumPublished data for WB in Dictyostelium discoideum vegetative cells. -SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Published data for IP in HEK293 cellsPublished data for IP in HEK293 cells. -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by from Published data for WB in mouse bone marrowPublished data for WB in mouse bone marrow. -SCBT Publication Review
Date published: 2015-02-19
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OctA-Probe 抗体 (F-tag-01) is rated 4.4 out of 5 by 9.
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