Date published: 2025-9-7
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Ob-R/Leptin Receptor Antibody (B-3) - Western Blotting - Image 376082
Ob-R 抗体 (B-3): sc-8391. MEG-01 全细胞裂解液中 Ob-R 表达的 Western 印迹分析.
Ob-R 抗体 (B-3): sc-8391. MEG-01 全细胞裂解液中 Ob-R 表达的 Western 印迹分析.

Ob-R/Leptin Receptor 抗体 (B-3): sc-8391

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说明书
  • Ob-R/Leptin Receptor 抗体 B-3 是小鼠单克隆 IgG1 κ，Ob-R/Leptin Receptor抗体, 在113篇文献中引用，规格为200 µg/ml
  • 与mouse的870-894氨基酸残基的the short form of Ob-R
  • Ob-R/Leptin Receptor 抗体 (B-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 short and long forms of Ob-R; 也和以下物种反应，包括： canine
  • 抗Ob-R/Leptin Receptor抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ob-R/Leptin Receptor (B-3): sc-8391的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Ob-R/Leptin Receptor 抗体 (B-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ob-R/Leptin Receptor 抗体 (B-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Ob-R抗体（B-3）是一种IgG1 κ小鼠单克隆Ob-R抗体（也称为LEPR抗体或瘦素受体抗体），可通过WB、IP、IF、IHC（P）和ELISA检测来自小鼠、大鼠和人类的Ob-R蛋白。Ob-R抗体（B-3）既可以是非偶联的抗Ob-R抗体形式，也可以是多种偶联形式的抗Ob-R抗体，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。尽管有大量证据表明体重是受生理调节的，但肥胖的分子基础尚不清楚。已经确定了五种导致肥胖表型的小鼠单基因突变。第一个这样的隐性肥胖突变，即肥胖突变（Ob），于1950年被确定。Ob突变会导致严重的肥胖和II型糖尿病，作为人类肥胖的一部分综合征。据推测，Ob基因产物可能作为脂肪组织中一个信号通路的组成部分，用于调节体内脂肪库的大小。最近描述了小鼠Ob基因及其人类同源基因的克隆和序列分析。Ob编码一种专一于脂肪组织的mRNA，具有高度保守的167个氨基酸开放阅读框架。预测的氨基酸序列在人类和小鼠之间相似度为84％，具有分泌蛋白的特征。在原始的C57BL/6J Ob/Ob小鼠株中发现了第105个密码子中的无意义突变。Ob基因编码蛋白瘦素。瘦素受体Ob-R已被证明是一种单膜跨膜受体，最类似于IL-6、G-CSF和LIF受体的gp130信号转导组分。Ob-R mRNA在脉络膜和下丘脑中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ob-R/Leptin Receptor 抗体 (B-3) 参考文献:

  1. 解决重大问题  |  Friedman, JM. and Leibel, RL. 1992. Cell. 69: 217-20. PMID: 1568242
  2. 小鼠 ob 突变的分子图谱。  |  Friedman, JM., et al. 1991. Genomics. 11: 1054-62. PMID: 1686014
  3. 患有干燥综合征的小鼠中瘦素/OB-R信号增强，与疾病发病机制有关。  |  Xu, T., et al. 2017. Biochem Biophys Res Commun. 482: 835-842. PMID: 27889607
  4. 瘦素/OB-R通路促进B淋巴细胞分泌IL-4，并诱导干燥综合征中唾液腺上皮细胞凋亡。  |  Xu, T., et al. 2017. Oncotarget. 8: 63417-63429. PMID: 28969001
  5. 早期乳腺癌患者接受新辅助全身治疗时瘦素受体（Ob-R）过度表达的预测作用。  |  García-Estévez, L., et al. 2021. Cancers (Basel). 13: PMID: 34210055
  6. 遗传学寻找饱腹感因子  |  Rink, TJ. 1994. Nature. 372: 406-7. PMID: 7984230
  7. 小鼠肥胖基因及其人类同源物的定位克隆。  |  Zhang, Y., et al. 1994. Nature. 372: 425-32. PMID: 7984236
  8. 瘦素和OB-R：细胞因子受体对体重的调节。  |  White, DW. and Tartaglia, LA. 1996. Cytokine Growth Factor Rev. 7: 303-9. PMID: 9023054

订购信息

产品名称产品编号规格价格数量收藏夹

Ob-R/Leptin Receptor 抗体 (B-3)

sc-8391
200 µg/ml
RMB2377.00

Ob-R/Leptin Receptor (B-3): m-IgG Fc BP-HRP 套装

sc-526514
200 µg Ab; 10 µg BP
RMB2662.00

Ob-R/Leptin Receptor (B-3): m-IgG1 BP-HRP 套装

sc-531887
200 µg Ab; 20 µg BP
RMB2662.00

Ob-R/Leptin Receptor 抗体 (B-3) AC

sc-8391 AC
500 µg/ml, 25% agarose
RMB3129.00

Ob-R/Leptin Receptor 抗体 (B-3) HRP

sc-8391 HRP
200 µg/ml
RMB2377.00

Ob-R/Leptin Receptor 抗体 (B-3) FITC

sc-8391 FITC
200 µg/ml
RMB2482.00

Ob-R/Leptin Receptor 抗体 (B-3) PE

sc-8391 PE
200 µg/ml
RMB2580.00

Ob-R/Leptin Receptor 抗体 (B-3) Alexa Fluor® 488

sc-8391 AF488
200 µg/ml
RMB2685.00

Ob-R/Leptin Receptor 抗体 (B-3) Alexa Fluor® 546

sc-8391 AF546
200 µg/ml
RMB2685.00

Ob-R/Leptin Receptor 抗体 (B-3) Alexa Fluor® 594

sc-8391 AF594
200 µg/ml
RMB2685.00

Ob-R/Leptin Receptor 抗体 (B-3) Alexa Fluor® 647

sc-8391 AF647
200 µg/ml
RMB2685.00

Ob-R/Leptin Receptor 抗体 (B-3) Alexa Fluor® 680

sc-8391 AF680
200 µg/ml
RMB2685.00

Ob-R/Leptin Receptor 抗体 (B-3) Alexa Fluor® 790

sc-8391 AF790
200 µg/ml
RMB2685.00

Ob-R/Leptin Receptor (B-3) 中和勝肽

sc-8391 P
100 µg/0.5 ml
RMB511.00

Hello, We are looking for an "ANIMAL-FREE" antibody against human Leptin Receptor to use in Immunoblot and/or Immunofluorescence. Is there an animal-free antibody that you would recommend?

Asked by: Rodriguez
Thank you for your question. Please contact technical service (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-03-16

请问有人用这个抗体直接做流式检测吗，效果如何

Asked by: kaia
Thank you for your question. We have not yet tested Ob-R/Leptin Receptor (B-3): sc-8391 on FCM. As a result, FCM is not currently a recommended application for this antibody. 感谢您的提问。我们还没有验证Ob-R/Leptin Receptor 抗体 (B-3): sc-8391在流式中的应用，所以暂时不推荐流式实验方法的检测。
Answered by: Yanru Tao
Date published: 2022-06-08

您好，我想问一下Ob-R (B-3)这个抗体针对的是Ob-R A/B/C/D/E/F的哪种亚型

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Hello, is this the same anti-leptin receptor antibody SC biotech sold in 2001? It worked great but gave conflicting results. I see that several anti-leptin receptor Abs were discontinued by SC, so wondering if this is the same or new?

Asked by: Ray4
Thank you for your question. This mouse monoclonal antibody has been available for a long time and was sold in 2001. It has not changed. Santa Cruz Biotechnology discontinued all of our polyclonal antibodies in 2016 and we now exclusively focus on development high quality monoclonal antibodies.
Answered by: Technical Support
Date published: 2020-07-09

Bonjour, Sur des applications en cytométrie de flux, seriez vous en mesure de me transmettre les conditions dans lesquelles cet anticorps a été utilisé (cellules perméabilisées? cellules figées à 4°C?....) ? Cordialement,

Asked by: AFGAP
Merci pour votre question. Anticorps Ob-R (B-3): sc-8391 n'a pas été testé en cytométrie en flux. Toutefois, vous pouvez trouver des informations sur cette utilisation dans la publication PMID 24098717. "Under what conditions can this antibody be used in flow cytometry?" Thank you for your question. Ob-R Antibody (B-3): sc-8391 has not been tested in flow cytometry. However, you can find information on this use in publication PMID 24098717.
Answered by: Tech Support Europe
Date published: 2019-09-13

Hi, is this antibody neutralizing which means I can use it to block leptin receptor without side effects?

Asked by: 1__1
Thank you for your question. No, sc-8391:Ob-R (B-3) is not recommended for neutralizing proteins.
Answered by: Technical Support
Date published: 2019-01-06

I would like to get a free sample of sc-8391, is that possible? I want to test it on human brain, I can share the pictures with you when it works. Best, Merel Rijnsburger, PhD

Asked by: MErel Rijnsburger
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

可以用作sc-8325的替代品吗与牛反应的做 WB实验

Asked by: meng123
感谢您的提问。关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-05-02
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Rated 2 out of 5 by from din´t work for either WB or IFtryed it for mouse brain tissue and it din´t work for immuno staining /perfused, PFA /Metoh fixed or for WB.
Date published: 2022-06-21
Rated 3 out of 5 by from It worked for WBwe used this antibody for WB 1:500 and worked quite well but with aspecific bands
Date published: 2022-02-07
Rated 4 out of 5 by from Ottimo in diluizione 1:50Ho testato il prodotto in IF su oociti di diverse specie animali. Allego foto di microscopia confocale 40x Protocollo utilizzato: Fissazione in PFA al 4% Pretrattamento in blocking per 4-5 ore (triton-X 0,1%, BSA 0,5%, Glicina 2%, Sodium Azide 0,2 % e Siero 2%) Incubazione primario over night a 4 °C 3 Lavaggi per 30' Incubazione secondario 2 ore a 37° 3 lavaggi per 30' . Montaggio in vetrino
Date published: 2021-08-04
Rated 4 out of 5 by from worked well in western blotthe antidody was tested on rat tissue samples and gave a nice band.
Date published: 2017-12-13
Rated 4 out of 5 by from works for immunohystochemistyI've been using it in both, free floating brain slices and ovary slices on glass slides. Perfect for DAB with ABC amplification: 1:1,000 gives very clear staining for both applications. Fluorescence is little bit trickier. I have to pre-incubate with superblock/serum for at least 10 hours at 0C to get rid of high background. Still, for 1:50 there is some background and 1:100 is too weak.
Date published: 2017-10-12
Rated 4 out of 5 by from 条带挺好的！效价还可以，效价不是很高，条带略微有点模糊，还可以勉强的使用。总体来说还是可以的，值得购买，包你满意！
Date published: 2017-10-01
Rated 3 out of 5 by from IHC Assay Review for Ob-R mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: Weak lymphocyte, neutrophil and monocyte staining at dilution factors 10, 30.
Date published: 2017-08-31
Rated 5 out of 5 by from good workingA month ago applied for this antibody, after preliminary experiments and experimental optimization has been satisfied with the results.
Date published: 2017-06-09
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Ob-R/Leptin Receptor 抗体 (B-3) is rated 4.4 out of 5 by 21.
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