Date published: 2025-9-11
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O-GlcNAc Antibody (CTD110.6) - Western Blotting - Image 17817
O-GlcNAc 抗体 (CTD110. 6): sc-59623. 对 HeLa 核提取物 (A), A549 全细胞裂解液 (B) 和小鼠脑组织提取物 (C) 中的 O-GlcNAc 修饰蛋白进行 Western 印迹分析.
O-GlcNAc Antibody (CTD110.6) - Western Blotting - Image 17817
O-GlcNAc 抗体 (CTD110. 6): sc-59623. 对 HeLa 核提取物 (A), A549 全细胞裂解液 (B) 和小鼠脑组织提取物 (C) 中的 O-GlcNAc 修饰蛋白进行 Western 印迹分析.
O-GlcNAc 抗体 (CTD110. 6): sc-59623. 对 HeLa 核提取物 (A), A549 全细胞裂解液 (B) 和小鼠脑组织提取物 (C) 中的 O-GlcNAc 修饰蛋白进行 Western 印迹分析.

O-GlcNAc 抗体 (CTD110.6): sc-59623

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寫評論提問

说明书
  • O-GlcNAc 抗体 CTD110.6 是小鼠单克隆 IgM κ，O-GlcNAc抗体, 在71篇文献中引用，规格为200 µg/ml
  • 与O-GlcNAc结合，含有丝氨酸-O-连接的N-乙酰葡糖胺
  • 推荐用于 a broad range of species, including mammals, insects, worms, plants 和 filamentous fungi 来源的Ser-O-GlcNAc and Thr-O-GlcNAc WB 和 IP检测; 不会与 peptide determinants or other closely-related carbohydrate antigens产生交联反应
  • 看 O-GlcNAc (RL2): sc-59624，可查看有标记的 O-GlcNAc 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取O-GlcNAc (CTD110.6): sc-59623的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成O-GlcNAc 抗体 (CTD110.6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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说明书与实验方案
研究信息

関連項目

此外 GlcNAc 抗体包括 O-GlcNAc

O-GlcNAc抗体（CTD110.6）是一种IgMκ小鼠单克隆O-GlcNAc抗体（也称为O-连接的β-N-乙酰葡糖胺抗体、O-β-GlcNAc抗体、O-连接的GlcNAb抗体、丝氨酸羟基O-GlcNAc抗体、苏氨酸羟基O-Glc NAc抗体或O-GlcNA酰化特异性抗体，植物和丝状真菌来源于WB和IP。O-GlcNAc抗体（CTD110.6）可作为非偶联的抗O-GlcNAc抗体。O-GlcNAc（O-连接的N-乙酰葡糖胺）是一种仅在真核细胞的细胞核和细胞质中发现的糖基化蛋白质。许多蛋白质的丝氨酸和苏氨酸羟基通过O-GlcNAc的连接而被修饰。调节进出核孔的蛋白质被广泛地O-GlcNA酰化。磷酸化的O-GlcNAc蛋白与其他蛋白形成可逆的多聚体复合物，并且这些结合通常由磷酸化调节。O-GlcNAc蛋白可能在肿瘤和各种癌症细胞的发病机制中发挥关键作用。O-GlcNAc残基调节起始前复合物的组装，因此在转录起始中是重要的。细胞骨架和膜O-GlcNAc蛋白维持红细胞形状，并调节阿尔茨海默病病变蛋白的降解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

O-GlcNAc 抗体 (CTD110.6) 参考文献:

  1. O-GlcNAc转移酶基因位于X染色体上，对胚胎干细胞的活力和小鼠的本体发育至关重要。  |  Shafi, R., et al. 2000. Proc Natl Acad Sci U S A. 97: 5735-9. PMID: 10801981
  2. O-GlcNAc转移酶和O-GlcNAc修饰蛋白在大鼠小脑皮质中的定位。  |  Akimoto, Y., et al. 2003. Brain Res. 966: 194-205. PMID: 12618343
  3. 核蛋白质和细胞质蛋白质的糖基化。尿苷二磷酸-N-乙酰葡糖胺：多肽 beta-N-乙酰葡糖胺基转移酶的纯化和表征。  |  Haltiwanger, RS., et al. 1992. J Biol Chem. 267: 9005-13. PMID: 1533623
  4. 确定参与梅花痘病毒感染的秘密因子为 O-GlcNAc 转移酶。  |  Chen, D., et al. 2005. J Virol. 79: 9381-7. PMID: 16014901
  5. O-GlcNAc 转移酶高通量测定法可检测肽底物的主序列偏好。  |  Leavy, TM. and Bertozzi, CR. 2007. Bioorg Med Chem Lett. 17: 3851-4. PMID: 17531489
  6. 磷酸肌醇信号将 O-GlcNAc 转移酶与胰岛素抵抗联系起来。  |  Yang, X., et al. 2008. Nature. 451: 964-9. PMID: 18288188
  7. O-GlcNAc转移酶：结构特征、催化机理和小分子抑制剂。  |  Ju Kim, E. 2020. Chembiochem. 21: 3026-3035. PMID: 32406185
  8. 蛋白质 O-GlcNAc 糖基化的化学探测进展：结构作用和分子机制》。  |  Saha, A., et al. 2021. Chem Soc Rev. 50: 10451-10485. PMID: 34338261
  9. 将 O-GlcNAc 纳入应激反应途径。  |  Fahie, KMM., et al. 2022. Cells. 11: PMID: 36359905
  10. GSDMD的O-GlcNAc修饰可减轻LPS诱导的内皮细胞脓毒症。  |  Yu, F., et al. 2024. Inflamm Res. 73: 5-17. PMID: 37962578
  11. 核蛋白质和细胞膜蛋白质的动态糖基化。具有多个四肽重复序列的独特 O-GlcNAc 转移酶的克隆和特征描述。  |  Kreppel, LK., et al. 1997. J Biol Chem. 272: 9308-15. PMID: 9083067
  12. O-Linked GlcNAc 转移酶是一种保守的核细胞质蛋白，含有四重肽重复序列。  |  Lubas, WA., et al. 1997. J Biol Chem. 272: 9316-24. PMID: 9083068

订购信息

产品名称产品编号规格价格数量收藏夹

O-GlcNAc 抗体 (CTD110.6)

sc-59623
200 µg/ml
RMB2377.00

I need specifically detect O-GlcNAcylation un plant proteim extractos. We found lack of specificity using CTD110.6. Employment of a secondary anti-IgM is mandatory? It could be conveniente to use RL2?

Asked by: Salamandra
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I am planning to IP this modification after protein digestion to peptides and wonder if the antibody recognise digested peptides carrying this modifications and if you would expect some specificity for certain peptides?

Asked by: Paolo
Thank you for your question. Unfortunately I do not have any information regarding if this antibody can detect digested peptides.
Answered by: Tech Service
Date published: 2019-04-09

What is the best way to avoid background when working on mouse samples with this mouse monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. Our new line of Mouse IgG binding proteins can help reduce non-specific staining with mouse samples. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety You can also use our alternative O-GlcNAc antibody sc-59624 in a variety of direct conjugations to reduce non-specific staining with mouse samples.
Answered by: Technical Service
Date published: 2016-12-22
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Rated 5 out of 5 by from goodvery nice，it work well in WB。Good specificity，nonspecific bands
Date published: 2021-07-31
Rated 4 out of 5 by from clear signal in immunofluorescence, dirty in WBThis antibody worked well in immunofluorescence in cell culture and also in paraffin embedded human brain tissue. In western blot, it needs a lot of washes after primary antibody especially, usually the first time there's a dark smear everywhere and if exposure is less than 5 min there's no signal. Usually after re-application of the secondary the signal gets clearer, perhaps due to longer washing of primary. Primary seems to stick, but this is a problem of this clone, not of this product in particular. Here are the captions again for the vertical (tissue) and horizontal (cells) IF pictures and the western blot since they did not fit in the space given: Cells: Immunofluorescence for O-GlcNAc in human astrocytes. A) no primary antibody. B) DMSO treatment (control). C) 200 uM Voc OGT inhibitor treatment. D) 100 uM 9D OGA inhibitor treatment. Green: O-GlcNAc (Santa Cruz CTD110.6), Blue: DAPI. Primary antibody was used at 1:100 dilution overnight in 5% goat serum in PBS. Secondary antibody was Goat anti-mouse IgM Alexafluor 488 conjugate. Tissue: Immunofluorescence in human tissue. A) IF of formalin fixed paraffin embedded human brain tissue. Control patient in his 60s. B) Alzheimer’s disease patient in his 60s. Green: O-GlcNAc. Blue: DAPI. Tissues were reparaffinized, rehydrated, antigen retrieval was performed in boiling citrate buffer, free formaldehydes were blocked with sodium borohydride. Blocking and antibody incubations were done in 5% goat serum in PBS. Lipofuscin was blocked with Sudan black for 20 min. after DAPI incubation. Western blot: Western blot with human astrocyte lysates. Multiple samples were run on SDS-PAGE gels and transferred to PVDF membrane. Blocking was with 5% milk in TBST. Primary antibody was used at 1:100 dilution in 5% BSA in TBST. Secondary antibody (rabbit anti-mouse IgM HRP) was diluted 1:1000 in 5% milk in TBST. Membrane was washed 2x 5 mins then 30 min in TBST after primary and then 3x 5 min after secondary. Imaging was for 10 mins.
Date published: 2020-12-30
Rated 5 out of 5 by from good result do WBWe used this antibody recenlty and following the struction choose the dilution 1:400(we feel it can diluted more),the band is right size,very good result.
Date published: 2018-05-26
Rated 4 out of 5 by from Worked wellI bought antibody for pilot experiments and it woked well in Western. I will be happy to buy more for future work.
Date published: 2018-02-16
Rated 5 out of 5 by from Really good !I used this antibody several times. It works well on human lung cells. even using a low dilution
Date published: 2017-07-05
Rated 5 out of 5 by from Great for Western blotMonoclonal O-GlcNAc (CTD110.6) antibody has been validated by Western blot. Highly recommended!
Date published: 2016-12-19
Rated 5 out of 5 by from Publishable WBPublishable WB.CoIP data with rat aortic smooth muscle cells - SCBT Publication Review
Date published: 2015-04-10
Rated 4 out of 5 by from Dirty blot but produced bands at expectedDirty blot but produced bands at expected MW in HeLa nuclear extract and A549 whole cell lysate and mouse brain tissue extract. -SCBT QC
Date published: 2015-03-26
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O-GlcNAc 抗体 (CTD110.6) is rated 4.7 out of 5 by 10.
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