Date published: 2025-9-6
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NY-ESO-1 Antibody (E978) - Western Blotting - Image 353989
NY-ESO-1 抗体 (E978): sc-53869. U266 (A) 和 MDA-MB-435S (B) 全细胞裂解液中 NY-ESO-1 表达的 Western 印迹分析.
NY-ESO-1 抗体 (E978): sc-53869. U266 (A) 和 MDA-MB-435S (B) 全细胞裂解液中 NY-ESO-1 表达的 Western 印迹分析.

NY-ESO-1 抗体 (E978): sc-53869

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说明书
  • NY-ESO-1 抗体 E978 是小鼠单克隆 IgG1 κ，NY-ESO-1抗体, 在41篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组NY-ESO-1
  • NY-ESO-1 抗体 (E978) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 NY-ESO-1
  • 抗NY-ESO-1抗体（E978）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NY-ESO-1 (E978): sc-53869的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 NY-ESO-1 抗体 (E978) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NY-ESO-1 抗体 (E978) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 NY-ESO 抗体包括 NY-ESO-1

NY-ESO-1 抗体（E978）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学方法检测人源 NY-ESO-1 蛋白。NY-ESO-1 (E978) 抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 共轭物。NY-ESO-1 又称纽约食管鳞状细胞癌 1，是肿瘤相关抗原癌症/睾丸（CT）家族的成员，主要在睾丸和卵巢中表达，因此 NY-ESO-1 是癌症免疫疗法的独特靶点。NY-ESO-1 在激发免疫反应方面起着至关重要的作用，因为 NY-ESO-1 经常被癌症患者的免疫系统识别，从而产生抗体并激活 CD8+ T 细胞对抗 HLA-A2 限制性表位。这种双重免疫反应意义重大，因为NY-ESO-1有可能成为治疗策略的靶点，旨在利用人体的免疫系统对抗各种癌症。NY-ESO-1 存在于多种肿瘤类型中，而在正常组织中的表达量有限，这突出了 NY-ESO-1 作为开发创新癌症治疗方法的候选靶点的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NY-ESO-1 抗体 (E978) 参考文献:

  1. NY-ESO-1 肿瘤缔合抗原是一种细胞质蛋白，可通过细胞系和临床标本中的特异性单克隆抗体检测到。  |  Schultz-Thater, E., et al. 2000. Br J Cancer. 83: 204-8. PMID: 10901371
  2. 监测任何 HLA I 类等全基因患者的 T 细胞对 NY-ESO-1 反应的策略。  |  Gnjatic, S., et al. 2000. Proc Natl Acad Sci U S A. 97: 10917-22. PMID: 11005863
  3. 恶性黑色素瘤疫苗接种：最新进展。  |  Jäger, D., et al. 2001. Oncology. 60: 1-7. PMID: 11150901
  4. 人类肝细胞癌中癌睾抗原的表达。  |  Chen, CH., et al. 2001. Cancer Lett. 164: 189-95. PMID: 11179834
  5. 使用在羧基末端修饰的 NY-ESO-1 多肽诱导肿瘤反应性细胞毒性 T 淋巴细胞，以增强 HLA-A2.1 在溶液中的结合亲和力和稳定性。  |  Bownds, S., et al. 2001. J Immunother. 24: 1-9. PMID: 11211143
  6. CD4(+) T 细胞识别由流行的 HLA DP4 等位基因呈现的 NY-ESO-1 的 MHC II 类限制表位：与 NY-ESO-1 抗体的产生有关。  |  Zeng, G., et al. 2001. Proc Natl Acad Sci U S A. 98: 3964-9. PMID: 11259659
  7. NY-ESO-1特异性TCR工程T细胞在骨髓瘤中介导持续的抗原特异性抗肿瘤效应。  |  Rapoport, AP., et al. 2015. Nat Med. 21: 914-921. PMID: 26193344
  8. 基于 NY-ESO-1 的癌症免疫疗法：当前视角。  |  Thomas, R., et al. 2018. Front Immunol. 9: 947. PMID: 29770138
  9. 内源性TCR敲除的NY-ESO-1特异性重定向T细胞介导肿瘤反应和细胞因子释放综合征。  |  Ishihara, M., et al. 2022. J Immunother Cancer. 10: PMID: 35768164
  10. NY-ESO-1特异性TCR工程化T细胞疗法联合淋巴结靶向纳米颗粒肽疫苗治疗晚期软组织肉瘤的1期试验。  |  Ishihara, M., et al. 2023. Int J Cancer. 152: 2554-2566. PMID: 36727538
  11. 评估 NY-ESO-1 特异性 TCR T 细胞对 HLA-A∗02:01 晚期软组织肉瘤患者的安全性和有效性的 1 期临床试验。  |  Pan, Q., et al. 2023. Cell Rep Med. 4: 101133. PMID: 37586317
  12. 癌症患者对一组人类肿瘤抗原的体液免疫反应调查。  |  Stockert, E., et al. 1998. J Exp Med. 187: 1349-54. PMID: 9547346

订购信息

产品名称产品编号规格价格数量收藏夹

NY-ESO-1 抗体 (E978)

sc-53869
200 µg/ml
RMB2377.00

NY-ESO-1 (E978): m-IgG Fc BP-HRP 套装

sc-528582
200 µg Ab; 10 µg BP
RMB2662.00

NY-ESO-1 (E978): m-IgGκ BP-HRP 套装

sc-520993
200 µg Ab, 40 µg BP
RMB2662.00

NY-ESO-1 (E978): m-IgG1 BP-HRP 套装

sc-543008
200 µg Ab; 20 µg BP
RMB2662.00

NY-ESO-1 抗体 (E978) AC

sc-53869 AC
500 µg/ml, 25% agarose
RMB3129.00

NY-ESO-1 抗体 (E978) HRP

sc-53869 HRP
200 µg/ml
RMB2377.00

NY-ESO-1 抗体 (E978) FITC

sc-53869 FITC
200 µg/ml
RMB2482.00

NY-ESO-1 抗体 (E978) PE

sc-53869 PE
200 µg/ml
RMB2580.00

NY-ESO-1 抗体 (E978) Alexa Fluor® 488

sc-53869 AF488
200 µg/ml
RMB2685.00

NY-ESO-1 抗体 (E978) Alexa Fluor® 546

sc-53869 AF546
200 µg/ml
RMB2685.00

NY-ESO-1 抗体 (E978) Alexa Fluor® 594

sc-53869 AF594
200 µg/ml
RMB2685.00

NY-ESO-1 抗体 (E978) Alexa Fluor® 647

sc-53869 AF647
200 µg/ml
RMB2685.00

NY-ESO-1 抗体 (E978) Alexa Fluor® 680

sc-53869 AF680
200 µg/ml
RMB2685.00

NY-ESO-1 抗体 (E978) Alexa Fluor® 790

sc-53869 AF790
200 µg/ml
RMB2685.00

Has anyone used this for flow cytometry and if so what dilution did you use?

Asked by: MrsCMJ
Thank you for your question. NY-ESO-1 Antibody (E978): sc-53869 is recommended for detection of NY-ESO-1 of human origin by WB, IP, IF and IHC(P). There are a couple of publications in which this antibody has been used to detect human NY-ESO-1. Their PMIDs are 20008301, 20730489, and 9505109. Feel free to contact our technical service directly if you require further assistance.
Answered by: Technical Support Europe
Date published: 2025-09-02

If I freeze this antibody, will it remain viable for a longer period of time?

Asked by: Trav11
Thank you for your question. We do not run long term storage testing, but warranty all of our products for 1 year from the date of purchase. Freezing the antibody will actually void the warranty as this harms the antibody due to the presence of gelatin in the suspension mixture. The recommended storage temperature is 4ºC and many times the antibody will remain viable under these conditions for much longer than the 1 year warranty date. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2016-12-29
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Rated 5 out of 5 by from Great functioning antibodyWe have tested the sample of this antibody in a section of formalin fixed, paraffin-embedded human testis tissue. Heat Induced Epitope Retrieval for 20 minutes at °C in TrisEDTA pH 9.0 Buffer with a 1:50 dilution of the antibody gave a clear contrast of positive germ line cells (see enclosed image).
Date published: 2018-04-11
Rated 5 out of 5 by from AlexaFluor 645 Conjugated PrimaryI used the NYESO1 AlexaFluor 645 Conjugated Primary to stain fixed 22Rv1 cells in suspension. It has worked great in a 1:50 dilution with binding buffer. I imaged with the Evos FL Auto 2 microscopy platform. Try it out!
Date published: 2017-09-29
Rated 5 out of 5 by from WB analysisWe detected the expression of NY-ESO-1 protein in 20 cases of Human liver cancer tissues by WB. This antibody is very helpful to our experimental analysis, and the antibody specificity and sensitive is very good . According to the literature reports, we used the fresh tissue of liver cancer as the positive control, and the sample 40ug was used,we obtained the target band at 22kDa .
Date published: 2017-09-09
Rated 5 out of 5 by from Excellent in many applicationsGreat, well-cited antibody that produces strong signals in multiple applications.
Date published: 2017-01-06
Rated 5 out of 5 by from Works very well by IHC(P)Works very well by IHC(P) - Published data
Date published: 2015-08-04
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear andExceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-05-21
Rated 4 out of 5 by from Strong band seen in WB using U266 nuclear extractStrong band seen in WB using U266 nuclear extract. -SCBT QC
Date published: 2015-04-29
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NY-ESO-1 抗体 (E978) is rated 4.9 out of 5 by 7.
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