Date published: 2025-9-7

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nucleobindin 2 抗体 (D-10): sc-376947

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说明书
  • nucleobindin 2 抗体 D-10 是小鼠单克隆 IgG1 κ,nucleobindin 2抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列347-420位于human物种的nucleobindin 2的C-terminus
  • nucleobindin 2 抗体 (D-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 nucleobindin 2
  • 抗nucleobindin 2抗体(D-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取nucleobindin 2 (D-10): sc-376947免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 nucleobindin 2 抗体 (D-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与nucleobindin 2 抗体 (D-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Nucleobindin 2抗体(D-10)是一种IgG1 κ小鼠单克隆Nucleobindin 2抗体(也称为Nucleobindin 2抗体),能够通过蛋白质印迹法(WB)、免疫沉淀法(IP)、免疫荧光法(IF)、免疫组织化学法(IHC(P))和酶联免疫吸附测定(ELISA)检测人类来源的Nucleobindin 2蛋白。Nucleobindin 2抗体(D-10)既有非偶联形式的抗Nucleobindin 2抗体,也有多种偶联形式的抗Nucleobindin 2抗体,包括琼脂糖、HRP、PE、FITC以及多种Alexa Fluor®偶联物。Nucleobindin 2,也称为NUCB2或NEFA,是一种含有420个氨基酸的蛋白质,主要在脾脏、睾丸和胃中表达。它定位于高尔基体和内质网(ER)的扁平囊,以及大脑中神经元的核膜上。Nucleobindin 2包含亮氨酸拉链和EF手性模体、两个螺旋-环-螺旋区域,以及一个碱性和一个酸性氨基酸区域。亮氨酸拉链结构和富含碱性氨基酸的区域负责DNA结合。它是一种高电荷蛋白质,通过其EF手性结构域结合Ca2+。Nucleobindin 2也在大鼠的下丘脑核中表达,这可能表明它在食欲控制中起作用。大脑中Nucleobindin 2向nesfatin-1的转化会减少大鼠的食物摄入量。Nesfatin-1被确定为一种饱腹分子,参与下丘脑中的黑皮质素信号传导。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

nucleobindin 2 抗体 (D-10) 参考文献:

  1. 人类 NEFA 的异源过表达以及对两个 EF 手钙质结合位点的研究。  |  Kroll, KA., et al. 1999. Biochem Biophys Res Commun. 260: 1-8. PMID: 10381334
  2. 凋亡后生长抑制因子 necdin 与神经细胞胞质中的钙结合蛋白(NEFA)相互作用。  |  Taniguchi, N., et al. 2000. J Biol Chem. 275: 31674-81. PMID: 10915798
  3. 绘制人类染色体 11p15.1-->p14 上基因丰富的 400-kb 区域的基因和转录序列图。  |  Caldwell, GM., et al. 2001. Cytogenet Cell Genet. 92: 103-7. PMID: 11306805
  4. 人类蛋白质 NEFA 在高尔基体中的保留是由其 N 端富含 Leu/Ile 的区域介导的。  |  Nesselhut, J., et al. 2001. FEBS Lett. 509: 469-75. PMID: 11749975
  5. 鉴定下丘脑中的饱腹感分子内司蛋白-1。  |  Oh-I, S., et al. 2006. Nature. 443: 709-12. PMID: 17036007
  6. [硬皮病患者的转运核糖核酸相关抗-Wa 抗体与目标抗原 NEFA/Nucleobindin-2].  |  Imura, Y. and Mimori, T. 2007. Nihon Rinsho Meneki Gakkai Kaishi. 30: 151-5. PMID: 17603255
  7. 大鼠下丘脑室旁核和视上核中的 Nesfatin-1 神经元共同表达催产素和血管加压素,并在进食后被激活。  |  Kohno, D., et al. 2008. Endocrinology. 149: 1295-301. PMID: 18048495
  8. Caspases 对 nucleobindin-1 和 nucleobindin-2 的调节。  |  Valencia, CA., et al. 2008. FEBS Lett. 582: 286-90. PMID: 18154733
  9. 核仁蛋白-2可增强肾细胞癌的上皮-间质转化。  |  Tao, R., et al. 2020. Oncol Lett. 19: 3653-3664. PMID: 32391090
  10. 核结合蛋白-2在癌变中的多面性。  |  Skorupska, A., et al. 2021. Int J Mol Sci. 22: PMID: 34073612
  11. 核结合蛋白-2在胶质母细胞瘤的临床发病机理和治疗耐受性中的作用。  |  Lin, IC., et al. 2023. Cells. 12: PMID: 37830634
  12. 人类蛋白质 NEFA,一种新型 DNA 结合/EF-手/亮氨酸拉链蛋白。cDNA 的分子克隆和序列分析, 蛋白质的分离和表征。  |  Barnikol-Watanabe, S., et al. 1994. Biol Chem Hoppe Seyler. 375: 497-512. PMID: 7811391

订购信息

产品名称产品编号规格价格数量收藏夹

nucleobindin 2 抗体 (D-10)

sc-376947
200 µg/ml
RMB2377.00

nucleobindin 2 (D-10): m-IgG Fc BP-HRP 套装

sc-527555
200 µg Ab; 10 µg BP
RMB2662.00

nucleobindin 2 (D-10): m-IgG1 BP-HRP 套装

sc-532928
200 µg Ab; 20 µg BP
RMB2662.00

nucleobindin 2 抗体 (D-10) AC

sc-376947 AC
500 µg/ml, 25% agarose
RMB3129.00

nucleobindin 2 抗体 (D-10) HRP

sc-376947 HRP
200 µg/ml
RMB2377.00

nucleobindin 2 抗体 (D-10) FITC

sc-376947 FITC
200 µg/ml
RMB2482.00

nucleobindin 2 抗体 (D-10) PE

sc-376947 PE
200 µg/ml
RMB2580.00

nucleobindin 2 抗体 (D-10) Alexa Fluor® 488

sc-376947 AF488
200 µg/ml
RMB2685.00

nucleobindin 2 抗体 (D-10) Alexa Fluor® 546

sc-376947 AF546
200 µg/ml
RMB2685.00

nucleobindin 2 抗体 (D-10) Alexa Fluor® 594

sc-376947 AF594
200 µg/ml
RMB2685.00

nucleobindin 2 抗体 (D-10) Alexa Fluor® 647

sc-376947 AF647
200 µg/ml
RMB2685.00

nucleobindin 2 抗体 (D-10) Alexa Fluor® 680

sc-376947 AF680
200 µg/ml
RMB2685.00

nucleobindin 2 抗体 (D-10) Alexa Fluor® 790

sc-376947 AF790
200 µg/ml
RMB2685.00

可以用于小鼠吗

Asked by: sssssss
感谢您的提问。sc-376947 暂不推荐用于小鼠来源的样本,但是您可以申请sc-376947 的试用装试试看。更多问题,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. sc-376947 is recommended for detection of nucleobindin 2 of human origin by WB, IP, IF, IHC(P) and ELISA now, but you can request for the free sample of this antibody to have a test. For more questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-11-15

I am using nucleobindin 2 (D-10): sc-376947 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25

请问到货大概需要多长时间?有没有试用装?

Asked by: 梁 运来
您好。中国大陆地区的产品订购及相关问题,请联系我们的上海分公司:电话(+86) 021-60936351,邮箱[email protected]。谢谢。 For questions regarding ordering in Mainland China, please contact our Shanghai branch at (+86) 021-60936351 or [email protected]. Thank you.
Answered by: Technical Support 14
Date published: 2017-02-06

抗体价格是279RMB1ml?

Asked by: 梁 运 来
您好。该抗体的人民币价格为RMB 2099,抗体为1 ml装。您可以在网页右上角选择货币形式。更多问题,请联系我们的上海分公司:电话(+86) 021-60936351,邮箱[email protected]。 Thank you for your question. 279 is the US dollar price of this antibody. You can select the preferable currency form in the upper right corner of the webpage. If you have further questions, please contact us at (+86) 021-60936351 or [email protected].
Answered by: Technical Support 14
Date published: 2017-02-06
  • y_2025, m_9, d_7, h_4CST
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Rated 5 out of 5 by from Excellent functionThis product is very good and very helpful to my experiment.I would recommend my colleague to buy this product too
Date published: 2017-08-07
Rated 5 out of 5 by from quite usefulI bought this antibody by January. It's quite useful.
Date published: 2017-05-15
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human upper stomach tissue. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Satisfactory Western Blot data of nucleobindinSatisfactory Western Blot data of nucleobindin 2 expression in HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2014-06-19
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nucleobindin 2 抗体 (D-10) is rated 5.0 out of 5 by 4.
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