Date published: 2025-9-6

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NT5C1A 抗体 (C-9): sc-377244

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说明书
  • NT5C1A 抗体 C-9 是小鼠单克隆 IgM κ,NT5C1A抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的NT5C1A的内部区域内的氨基酸163-195之间
  • 推荐用于 mouse, rat 和 human 来源的NT5C1A WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: 和 equine and canine
  • 关于如何获取NT5C1A (C-9): sc-377244免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成NT5C1A 抗体 (C-9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

NT5C1A抗体(C-9)是一种IgM κ小鼠单克隆NT5C1A抗体(也称为NT5C1A抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源性NT5C1A蛋白。NT5C1A抗体(C-9)为非偶联型抗NT5C1A抗体。NT5C1A(胞质5'-核苷酸酶1A)是一种属于5'-核苷酸酶3型的由365个氨基酸组成的蛋白质。NT5C1A在ADP的激活下,利用镁和水使脱氧核糖核苷酸的5'和2'(3')-磷酸去磷酸化,生成核糖核苷和磷酸。NT5C1A定位于细胞质,具有广泛的底物特异性。NT5C1A还辅助调节缺血和缺氧时心脏中的腺苷水平。编码NT5C1A的基因位于4号染色体上,该染色体包含近900个基因,约占人类基因组的6%。据报道,4号染色体含有最大的基因沙漠(即基因组中没有蛋白质编码基因的区域),并且是人类染色体中重组频率最低的两条染色体之一。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NT5C1A 抗体 (C-9) 参考文献:

  1. 睾丸单倍体生殖细胞特异基因 Oxct2a 和 Oxct2b 的基因结构和进化。  |  Onishi, M., et al. 2004. Genomics. 83: 647-57. PMID: 15028287
  2. 人类 2 号和 4 号染色体 DNA 序列的生成和注释。  |  Hillier, LW., et al. 2005. Nature. 434: 724-31. PMID: 15815621
  3. 细胞质 IMP-GMP 特异性 5'-核苷酸酶 II(cN-II)结构和功能的最新进展。  |  Ipata, PL. and Tozzi, MG. 2006. Purinergic Signal. 2: 669-75. PMID: 18404470
  4. 人骨骼肌中细胞膜 5'核苷酸酶的表达与氧化代谢标志物肌红蛋白的表达无关。  |  Lechward, K. and Tkacz-Stachowska, K. 2009. Acta Biochim Biophys Sin (Shanghai). 41: 280-4. PMID: 19352542
  5. 瑞舒伐他汀可增加人体内细胞外腺苷的形成:保护心血管的新视角。  |  Meijer, P., et al. 2009. Arterioscler Thromb Vasc Biol. 29: 963-8. PMID: 19359665

订购信息

产品名称产品编号规格价格数量收藏夹

NT5C1A 抗体 (C-9)

sc-377244
200 µg/ml
RMB2377.00

NT5C1A (C-9) 中和勝肽

sc-377244 P
100 µg/0.5 ml
RMB511.00

For human cell lysate which blocking buffer do you suggest for this Ab? And the staining time? I tried 3h staining in either 5% milk or 3% bsa but I don't get the signal. thank you

Asked by: ElenaD
Thank you for your question. We recommend the plant-based UltraCruz® Blocking Reagent: sc-516214. Please also note the antibody's recommended dilution range in its datasheet and feel free to reach out to technical service if the issue persists.
Answered by: Tech Support Europe
Date published: 2021-03-08

I would like to use the NT5c1A (c-9) antibody for frozen human skeletal muscle specimen. Do you have experiences on frozen tissue ? Thank you very much.

Asked by: Endre
Thank you for your question. NT5C1A Antibody (C-9): sc-377244 is recommended for the detection of NT5C1A of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine and canine. Based on our tests with this antibody, we do not recommend it for detection of its epitope in IHC applications.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What application is the blocking peptide sc-377244 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-18
Rated 5 out of 5 by from Produced positive Western Blot data of NT5C1AProduced positive Western Blot data of NT5C1A expression in A549 whole cell lysate. -SCBT QC
Date published: 2014-08-22
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NT5C1A 抗体 (C-9) is rated 5.0 out of 5 by 2.
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