Date published: 2025-9-7
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Nrf2 Antibody (437C2a) - Western Blotting - Image 16723
Nrf2 抗体 (437C2a): sc-81342 人重组 Nrf2 融合蛋白的 Western 印迹分析.
Nrf2 Antibody (437C2a) - Western Blotting - Image 16723
Nrf2 抗体 (437C2a): sc-81342 人重组 Nrf2 融合蛋白的 Western 印迹分析.
Nrf2 抗体 (437C2a): sc-81342 人重组 Nrf2 融合蛋白的 Western 印迹分析.

Nrf2 抗体 (437C2a): sc-81342

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说明书
  • Nrf2抗体(437C2a)是小鼠单克隆IgG1 κ, 在29篇文献中引用，规格为100 µg/ml
  • 针对与human起源的Nrf2的内部区域对应的重组蛋白
  • 推荐用于 human 来源的Nrf2 WB 和 IP检测
  • 看 Nrf2 (A-10): sc-365949，可查看有标记的 Nrf2 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成Nrf2 抗体 (437C2a)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

附加Nuclear Factor Erythroid 2-Related Factor抗体，包括Nrf1和Nrf2

NF-E2 DNA结合蛋白由两个亚基p45和MafK组成。它通过与Maf识别元件(Mares)相互作用调节发育中红细胞中珠蛋白基因的表达。NF-E2相关蛋白家族统称为Cap 'n' collar (CNC)家族，包括Nrf1（也称为TCF11）、Nrf2和Nrf3，它们是bZIP转录因子，与Maf蛋白异二聚体结合以结合Mare序列。Nrf蛋白还结合抗氧化反应元件(ARE)，并涉及解毒酶和氧化应激反应的调节。它们通过与Jun家族成员（c-Jun、Jun B和Jun D）异二聚体化来激活基因表达，特别是解毒酶NQO1。Nrf2广泛表达，在用异生素和抗氧化剂处理后，被认为会转移到细胞核中，这会刺激其从阻遏蛋白Keap1中释放。在免疫印迹应用中，Nrf2抗体（A-10）可检测全细胞裂解液和核提取物中的Nrf2蛋白。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Nrf2 抗体 (437C2a)

sc-81342
100 µg/ml
RMB2504.00

At which band is Nrf2 supposed to appear? is it 40KD (according to the figure) or 61KD (according to the molecular weight)??

Asked by: Khal24
Thank you for your question. The Western blot data published at 40 kDa with this antibody is of a human recombinant Nrf2 fusion protein and is not indicative of the endogenous molecular weight of Nrf2. Please see a useful reference for the molecular weight of Nrf2 below. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503463/
Answered by: Technical Support
Date published: 2019-02-10

I am using Nrf2 (437C2a): sc-81342 antibody for immunoprecipitation, how much antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 2 out of 5 by from ConfusingIts not showing the band at the correct molecular weight
Date published: 2018-03-07
Rated 2 out of 5 by from Bandas inespecíficasProbé el anticuerpo Nrf2 437C2a en un WB con la línea celular HEPG2,al mismo tiempo que el anticuerpo Nrf2 (A-10): sc-365949. Con el 437C2a aparecen muchas bandas inespecíficas, es mejor el A-10
Date published: 2018-02-22
Rated 5 out of 5 by from Beautiful strong single bandOur laboratory got this in replacement of the old ab we had. Glad we got this, did it once and didn't have to repeat the experiment.
Date published: 2017-11-12
Rated 5 out of 5 by from Reliable resultReliable result by western blot detect Nrf2 ,I suggest dilution 1:800,strong band wasdeteced at 40kda, I suggest use positive control for detection.
Date published: 2017-09-09
Rated 5 out of 5 by from very good antibody for human samplesThis is a very good antibody for human samples, and we will use it for human applications.
Date published: 2017-02-12
Rated 5 out of 5 by from 1:1000 for best resultsFor best results dilute primary antibody 1:1000 and secondary antibody 1:5000 and incubate for just 1 hour with each.
Date published: 2017-02-12
Rated 5 out of 5 by from GreatThis production is very great with high quanlity and high specificity.
Date published: 2016-11-19
Rated 3 out of 5 by from Strong band in WB with human recombinantStrong band in WB with human recombinant Nrf2 fusion protein. -SCBT QC
Date published: 2014-11-02
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Nrf2 抗体 (437C2a) is rated 4.0 out of 5 by 8.
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