Date published: 2025-9-7

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Nrf1 抗体 (G-5): sc-515360

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说明书
  • Nrf1 抗体 G-5 是小鼠单克隆 IgG2b, 在5篇文献中引用,提供 200 µg/ml
  • 特异性抗原位于human物种的Nrf1的C-terminus的氨基酸747-772之间
  • 抗-Nrf1 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Nrf1
  • 抗Nrf1抗体(G-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-515360 X, 200 µg/0.1 ml)
  • 关于如何获取Nrf1 (G-5): sc-515360免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是Nrf1 Antibody (G-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Nrf1 Antibody (G-5) 打包提供(请参阅下面的订购信息)。

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関連項目

Nrf1抗体(G-5)是一种小鼠单克隆IgG2b抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的Nrf1。Nrf1单克隆抗体(G-5)特异性识别人类Nrf1 C端747-772氨基酸之间的表位。抗Nrf1抗体(G-5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Nrf1(也称为TCF11)通过调节解毒酶和抗氧化蛋白的表达,在细胞防御机制中发挥着关键作用,从而促进细胞对氧化应激的反应。Nrf1属于Cap 'n' collar(CNC)转录因子家族,该家族还包括Nrf2和Nrf3,其特点是具有碱性亮氨酸拉链(bZIP)结构域,可促进与Maf蛋白的二聚化,从而与特定的DNA序列结合,这些序列被称为Maf识别元件(MAREs)和抗氧化反应元件(AREs)。Nrf1可与Jun家族成员(如c-Jun、JunB和JunD)形成异源二聚体,从而增强基因表达的激活,特别是解毒酶NAD(P)H:醌氧化还原酶1(NQO1)的激活。了解Nrf1的功能至关重要,因为这种蛋白质不仅调节参与解毒的基因,还在维持细胞稳态和保护细胞免受氧化损伤方面发挥重要作用,而氧化损伤与包括癌症和神经退行性疾病在内的多种疾病有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Nrf1 抗体 (G-5)

sc-515360
200 µg/ml
RMB2377.00

Nrf1 (G-5): m-IgG2b BP-HRP 套装

sc-548817
200 µg Ab; 10 µg BP
RMB2662.00

Nrf1 抗体 (G-5) X

sc-515360 X
200 µg/0.1 ml
RMB2377.00

Nrf1 抗体 (G-5) AC

sc-515360 AC
500 µg/ml, 25% agarose
RMB3129.00

Nrf1 抗体 (G-5) HRP

sc-515360 HRP
200 µg/ml
RMB2377.00

Nrf1 抗体 (G-5) FITC

sc-515360 FITC
200 µg/ml
RMB2482.00

Nrf1 抗体 (G-5) PE

sc-515360 PE
200 µg/ml
RMB2580.00

Nrf1 抗体 (G-5) Alexa Fluor® 488

sc-515360 AF488
200 µg/ml
RMB2685.00

Nrf1 抗体 (G-5) Alexa Fluor® 546

sc-515360 AF546
200 µg/ml
RMB2685.00

Nrf1 抗体 (G-5) Alexa Fluor® 594

sc-515360 AF594
200 µg/ml
RMB2685.00

Nrf1 抗体 (G-5) Alexa Fluor® 647

sc-515360 AF647
200 µg/ml
RMB2685.00

Nrf1 抗体 (G-5) Alexa Fluor® 680

sc-515360 AF680
200 µg/ml
RMB2685.00

Nrf1 抗体 (G-5) Alexa Fluor® 790

sc-515360 AF790
200 µg/ml
RMB2685.00

Nrf1 (G-5) 中和勝肽

sc-515360 P
100 µg/0.5 ml
RMB511.00

Is the blocking peptide sc-515360P equivalent to the peptide against which the antibody was raised or is it a truncation thereof? If the latter is the case, which sequence does peptide sc-515360P have?

Asked by: Questionasker01
Thank you for your question. The sc-516360P are peptides comprised the sequences used to generate the antibody and are incubated with the antibody in great excess. The exact sequence is considered to be proprietary information. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

What application is the blocking peptide sc-515360 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from 良く染まります!IFに使いました。核内移行についての評価をしていたのですが、 期待以上に良く染まり評価がしやすかったです。今後も継続して 使用させて頂きたいと思います。
Date published: 2018-02-08
Rated 4 out of 5 by from works well for western blottingrecognized a single band of the right size on western blotting of mouse whole cell extract at 1:1000 dilution with 3% BSA in PBST.
Date published: 2017-12-06
Rated 4 out of 5 by from great AntibodyAmount used WB was 1:500 in BSA and 1:7500 of secondary antibody on mitochondrial extracts
Date published: 2017-08-16
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Nrf1 抗体 (G-5) is rated 4.3 out of 5 by 3.
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