Date published: 2025-9-8

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NRAMP 2 抗体 (G-5): sc-166884

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说明书
  • NRAMP 2 抗体 G-5 是小鼠单克隆 IgG1 κ,NRAMP 2抗体, 在19篇文献中引用,规格为200 µg/ml
  • 针对氨基酸461-568的抗原,在NRAMP 2的C-terminus处映射,human来源
  • NRAMP 2 抗体 (G-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 NRAMP 2
  • 抗NRAMP 2抗体(G-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NRAMP 2 (G-5): sc-166884免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 NRAMP 2 抗体 (G-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NRAMP 2 抗体 (G-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

NRAMP 2抗体(G-5)是一种IgG1 κ小鼠单克隆NRAMP 2抗体(也称为NRAMP 2抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的NRAMP 2蛋白。 NRAMP 2抗体(G-5)可作为非偶联抗NRAMP 2抗体形式以及多种偶联形式的抗NRAMP 2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。天然抗性相关巨噬细胞蛋白(NRAMP)属于高度保守的膜内蛋白超家族。 NRAMP 1是一种位于吞噬体膜上的细胞内巨噬细胞蛋白,其功能是作为Fe2+、Zn2+和Mn2+的二价阳离子转运蛋白。 NRAMP 1是一种pH依赖性逆向转运蛋白,可逆向运输金属离子进出吞噬体。编码人NRAMP 1的基因位于染色体2q35。在人类中,NRAMP 1基因的多态性与结核分枝杆菌和麻风病的易感性有关。 NRAMP 2是另一种广泛表达的二价阳离子转运蛋白,以两种剪接变异体形式存在,其区别在于铁应答元件的存在(亚型1)或缺失(亚型2)。在小肠的十二指肠中,膳食铁调节刷状缘的NRAMP 2表达。编码人NRAMP 2的基因位于染色体12q13.12。小鼠和大鼠NRAMP 2基因突变导致严重贫血。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NRAMP 2 抗体 (G-5) 参考文献:

  1. 铁转运蛋白NRAMP2是一种整合膜糖蛋白,与转铁蛋白在回收内体中共同定位。  |  Gruenheid, S., et al. 1999. J Exp Med. 189: 831-41. PMID: 10049947
  2. Nramp2 铁转运体在肠刷状边界的细胞和亚细胞定位以及膳食铁的调控。  |  Canonne-Hergaux, F., et al. 1999. Blood. 93: 4406-17. PMID: 10361139
  3. 自然抗性相关巨噬细胞蛋白 1 是一种 H+/二价阳离子反转运体。  |  Goswami, T., et al. 2001. Biochem J. 354: 511-9. PMID: 11237855
  4. 从 Bcg/Lsh/Ity 到 Nramp1:三十年的探索与研究。  |  Buschman, E. and Skamene, E. 2001. Drug Metab Dispos. 29: 471-3. PMID: 11259334
  5. NRAMP1 基因与几内亚-科纳克里结核病易感性之间的等全基因关联。  |  Cervino, AC., et al. 2000. Ann Hum Genet. 64: 507-12. PMID: 11281214
  6. 克隆和鉴定染色体 12q13 上的第二个人类 NRAMP 基因。  |  Vidal, S., et al. 1995. Mamm Genome. 6: 224-30. PMID: 7613023
  7. 人类天然抵抗力相关巨噬细胞蛋白:cDNA 克隆, 染色体图谱, 基因组组织和组织特异性表达。  |  Cellier, M., et al. 1994. J Exp Med. 180: 1741-52. PMID: 7964458
  8. 麻风病的易感性与人类 NRAMP1 基因有关。  |  Abel, L., et al. 1998. J Infect Dis. 177: 133-45. PMID: 9419180
  9. 西非人 NRAMP1 基因变异与结核病易感性。  |  Bellamy, R., et al. 1998. N Engl J Med. 338: 640-4. PMID: 9486992
  10. 人类 Nramp2 基因:基因结构, 替代剪接, 启动子区域和多态性的特征。  |  Lee, PL., et al. 1998. Blood Cells Mol Dis. 24: 199-215. PMID: 9642100
  11. G185R 突变会破坏铁转运体 Nramp2 的功能。  |  Su, MA., et al. 1998. Blood. 92: 2157-63. PMID: 9731075
  12. 人类天然抵抗相关巨噬细胞蛋白2:基因克隆和蛋白质鉴定。  |  Kishi, F. and Tabuchi, M. 1998. Biochem Biophys Res Commun. 251: 775-83. PMID: 9790986

订购信息

产品名称产品编号规格价格数量收藏夹

NRAMP 2 抗体 (G-5)

sc-166884
200 µg/ml
RMB2377.00

NRAMP 2 (G-5): m-IgG Fc BP-HRP 套装

sc-529078
200 µg Ab; 10 µg BP
RMB2662.00

NRAMP 2 (G-5): m-IgGκ BP-HRP 套装

sc-521784
200 µg Ab, 40 µg BP
RMB2662.00

NRAMP 2 (G-5): m-IgG1 BP-HRP 套装

sc-543308
200 µg Ab; 20 µg BP
RMB2662.00

NRAMP 2 抗体 (G-5) AC

sc-166884 AC
500 µg/ml, 25% agarose
RMB3129.00

NRAMP 2 抗体 (G-5) HRP

sc-166884 HRP
200 µg/ml
RMB2377.00

NRAMP 2 抗体 (G-5) FITC

sc-166884 FITC
200 µg/ml
RMB2482.00

NRAMP 2 抗体 (G-5) PE

sc-166884 PE
200 µg/ml
RMB2580.00

NRAMP 2 抗体 (G-5) Alexa Fluor® 488

sc-166884 AF488
200 µg/ml
RMB2685.00

NRAMP 2 抗体 (G-5) Alexa Fluor® 546

sc-166884 AF546
200 µg/ml
RMB2685.00

NRAMP 2 抗体 (G-5) Alexa Fluor® 594

sc-166884 AF594
200 µg/ml
RMB2685.00

NRAMP 2 抗体 (G-5) Alexa Fluor® 647

sc-166884 AF647
200 µg/ml
RMB2685.00

NRAMP 2 抗体 (G-5) Alexa Fluor® 680

sc-166884 AF680
200 µg/ml
RMB2685.00

NRAMP 2 抗体 (G-5) Alexa Fluor® 790

sc-166884 AF790
200 µg/ml
RMB2685.00

For hum placental lysate I obtained 2 bands for DMT1, a strong signal around 50kDa and a weak band between 70 and 60kDa. Was wondering which one to quantify. Expected mol wt is 64kDa, which correspond to the weak signal in my blot betwn 50-70

Asked by: nikhitha
Thank you for your question. Please contact technical support at (800)-457-3801 or email [email protected]
Answered by: BlakeJ
Date published: 2023-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-02-03
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Rated 4 out of 5 by from Works well in WBIt worked well in WB. Bands were seen using human intestinal cell lines.
Date published: 2023-06-21
Rated 5 out of 5 by from Davvero soddisfacente per il wbL'anticorpo è stato testato 1:1000 su cellule Caco-2, le bande sono nette e i segnali ottimi! Lo consiglio
Date published: 2021-09-28
Rated 4 out of 5 by from Good for western blotI tried this antibody with whole cell lysate from human cell line. A clear band was detected by western blot.
Date published: 2017-12-19
Rated 5 out of 5 by from Very clear band and strong signalingI can get specific band with strong signaling in mouse liver samples using 1:2000 dilution
Date published: 2017-08-09
Rated 5 out of 5 by from Strong NRAMP 2 detectionGreat antibody with strong bands in Western blot for multiple cell lines and tissue types. Tested well in IHC(P) as well. Recommended for WB, IP, IF, IHC(P), and ELISA.
Date published: 2017-02-03
Rated 5 out of 5 by from extraordinary workingi brought this antibody 2 months ago i was facing problem with nramp2 antibody other companies now my problem was cleared
Date published: 2017-01-05
Rated 4 out of 5 by from Strong bands detected in WB using J774Strong bands detected in WB using J774.A1, RAW 264.7, NAMALWA and Hep G2 whole cell lysates and human liver tissue extract. -SCBT QC
Date published: 2015-05-21
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-05-08
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NRAMP 2 抗体 (G-5) is rated 4.5 out of 5 by 11.
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