Date published: 2025-9-6
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NPR-A Antibody (G-2) - Western Blotting - Image 55420
NPR-A 抗体 (G-2): sc-137041. KNRK 全细胞裂解液中 NPR-A 表达的 Western 印迹分析.
NPR-A 抗体 (G-2): sc-137041. KNRK 全细胞裂解液中 NPR-A 表达的 Western 印迹分析.

NPR-A 抗体 (G-2): sc-137041

3.5(6)
寫評論提問

说明书
  • NPR-A 抗体 G-2 是小鼠单克隆 IgG1 κ，NPR-A抗体, 在6篇文献中引用，规格为200 µg/ml
  • 免疫human物种的NPR-A的氨基酸141-265
  • NPR-A 抗体 (G-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 NPR-A
  • 抗NPR-A抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NPR-A (G-2): sc-137041的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 NPR-A 抗体 (G-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与NPR-A 抗体 (G-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Natriuretic Peptide Receptor 抗体包括 NPR-A, NPR-B 和 NPR-C

NPR-A抗体（G-2）是一种IgG1 κ小鼠单克隆NPR-A抗体（也称为NPR1抗体、鸟苷酸环化酶A（GC-A）抗体或ANPRA抗体），它通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的NPR-A蛋白。NPR-A抗体（G-2）既可作为非共轭抗NPR-A抗体形式，也可作为多种共轭形式的抗NPR-A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。钠尿肽是一组结构相似但遗传上不同的肽，在心血管、肾脏和内分泌稳态等多个过程中发挥作用。心房钠尿肽（ANP）和脑钠尿肽（BNP）来源于心肌细胞，是分别由心脏的心房和心室分泌的心脏激素。C型钠尿肽（CNP）来源于内皮细胞，起内皮源性舒张因子（EDRF）的作用。这些肽通过三种受体介导其作用。NPR-A（也称为GC-A）与ANP和BNP结合，刺激3′，5′-环鸟苷酸（cGMP）介导钠尿、血管扩张、肾素抑制、抗有丝分裂和lusitropic特性。NPR-B（也称为GC-B）与CNP结合，也刺激cGMP以促进血管扩张和生长抑制。NPR-C，也称为"清除"受体，清除随后被外切酶中性内肽酶降解的所有三种肽。钠尿肽系统在高血压、充血性心力衰竭、动脉粥样硬化和肾脏疾病中起重要作用，可能是这些疾病治疗的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NPR-A 抗体 (G-2) 参考文献:

  1. 对苯甲酰基[N,C,rANP(1-28)]pBNP32（pBNP1）衍生物的开发及牛 NPR-A 受体的亲和光标记。  |  Coupal, M., et al. 1999. Biochem Biophys Res Commun. 258: 81-6. PMID: 10222239
  2. 心力衰竭中的利钠肽：诊断和治疗潜力。  |  Chen, HH. and Burnett, JC. 1999. Proc Assoc Am Physicians. 111: 406-16. PMID: 10519161
  3. 鸟苷酸环化酶-B 是松果体中表达水平极高的主要利钠肽受体。  |  Müller, D., et al. 2000. Brain Res Mol Brain Res. 75: 321-9. PMID: 10686354
  4. 充血性心力衰竭病理生理学中的钠尿肽。  |  Chen, HH. and Burnett, JC. 2000. Curr Cardiol Rep. 2: 198-205. PMID: 10980893
  5. [钠尿肽系统的分子生物学和药理学]。  |  Itoh, H., et al. 1993. Nihon Rinsho. 51: 1548-56. PMID: 8100590
  6. 高血压中的心房利钠肽-C 受体和膜信号传导。  |  Anand-Srivastava, MB. 1997. J Hypertens. 15: 815-26. PMID: 9280203
  7. [钠尿肽系统]。  |  Itoh, H. and Nakao, K. 1997. Nihon Rinsho. 55: 1923-36. PMID: 9284403

订购信息

产品名称产品编号规格价格数量收藏夹

NPR-A 抗体 (G-2)

sc-137041
200 µg/ml
RMB2377.00

NPR-A (G-2): m-IgG1 BP-HRP 套装

sc-531671
200 µg Ab; 20 µg BP
RMB2662.00

NPR-A 抗体 (G-2) AC

sc-137041 AC
500 µg/ml, 25% agarose
RMB3129.00

NPR-A 抗体 (G-2) HRP

sc-137041 HRP
200 µg/ml
RMB2377.00

NPR-A 抗体 (G-2) FITC

sc-137041 FITC
200 µg/ml
RMB2482.00

NPR-A 抗体 (G-2) PE

sc-137041 PE
200 µg/ml
RMB2580.00

NPR-A 抗体 (G-2) Alexa Fluor® 488

sc-137041 AF488
200 µg/ml
RMB2685.00

NPR-A 抗体 (G-2) Alexa Fluor® 546

sc-137041 AF546
200 µg/ml
RMB2685.00

NPR-A 抗体 (G-2) Alexa Fluor® 594

sc-137041 AF594
200 µg/ml
RMB2685.00

NPR-A 抗体 (G-2) Alexa Fluor® 647

sc-137041 AF647
200 µg/ml
RMB2685.00

NPR-A 抗体 (G-2) Alexa Fluor® 680

sc-137041 AF680
200 µg/ml
RMB2685.00

NPR-A 抗体 (G-2) Alexa Fluor® 790

sc-137041 AF790
200 µg/ml
RMB2685.00

What are the conditions for Western Blot electrophoresis and electroporation of NPR-A antibody ? How much is the general concentration ratio suitable ? Why do I buy antibodies according to 1 : 1000 and 1 ; 100 have tried, the result is blank ?

Asked by: z wen
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-08-29

Can NPR-A (G-2): sc-137041 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, NPR-A (G-2): sc-137041 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Did not work in WesternThis antibody did not work in a Western on full-length recombinant protein. In contrast, sc-100380 and sc-377494 worked on that protein in the same Western. Secondary antibody was HRP-goat anti-mouse IgG (H+L) polyclonal.
Date published: 2018-07-20
Rated 1 out of 5 by from could not detectI received this as a sample to test, but could not detect in mouse brown adipose tissue lysates (20 ug/lane). Above 50 kD was blank on the blot. Run under reducing and denaturing conditions. Probed at 1:100 dilution overnight at 4C. Imaged with Li-Cor Odyssey system, which typically has difficulty picking up low signal. Perhaps HRP secondary method could work.
Date published: 2017-10-31
Rated 4 out of 5 by from Well done!I am surprised to see a beautiful band！！！！thanks a lot
Date published: 2017-03-15
Rated 5 out of 5 by from Good for IHCThis antibody provides strong staining in IHC tissue samples, even paraffin-embedded sections.
Date published: 2017-01-18
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from Nice Western Blot data of NPRNice Western Blot data of NPR-A expression in KNRK whole cell lysate. -SCBT QC
Date published: 2014-03-07
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NPR-A 抗体 (G-2) is rated 3.5 out of 5 by 6.
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