Date published: 2025-9-8
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Nogo Antibody (C-4) - Western Blotting - Image 137676
Nogo 抗体 (C-4): sc-271878. 非转染 293: sc-110760 (A), 人 Nogo 转染 293: sc-111336 (B), TE671 (C) 和 HeLa (D) 全细胞裂解液中 Nogo 表达的 Western 印迹分析.
Nogo 抗体 (C-4): sc-271878. 非转染 293: sc-110760 (A), 人 Nogo 转染 293: sc-111336 (B), TE671 (C) 和 HeLa (D) 全细胞裂解液中 Nogo 表达的 Western 印迹分析.

Nogo 抗体 (C-4): sc-271878

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说明书
  • Nogo 抗体 C-4 是小鼠单克隆 IgM κ，Nogo抗体, 在27篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Nogo的N-terminus的氨基酸18-41之间
  • 推荐用于 mouse, rat 和 human 来源的Nogo A, Nogo B and foocen WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： porcine
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取Nogo (C-4): sc-271878的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Nogo Antibody (C-4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与Nogo Antibody (C-4) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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研究信息

関連項目

此外 Nogo 抗体包括 Nogo A

Nogo抗体（C-4）是一种IgM κ小鼠单克隆Nogo抗体（也称为神经突生长抑制剂（Nogo）抗体，或神经内分泌分泌调节蛋白抗体），可通过WB、IP、IF、IHC（P）和ELISA检测来自小鼠、大鼠和人类的Nogo蛋白。Nogo抗体（C-4）既有非偶联抗Nogo抗体形式，也有多种偶联形式的抗Nogo抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。中枢神经系统白质选择性抑制轴突生长。Nogo（也称为NI250和Reticulon 4-A）是Reticulon家族的少突胶质细胞特异性成员，是抑制轴突生长的中枢神经系统白质成分，可诱导鸡背根神经节细胞生长锥的塌陷，并抑制3T3成纤维细胞的扩散。网状蛋白家族的其他成员不抑制轴突延伸，被认为在ER功能中发挥作用。Nogo由少突胶质细胞表达，而不由雪旺细胞表达，主要与内质网相关。Nogo有三种不同的剪接形式，Nogo-A、Nogo-B和Nogo-C。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nogo 抗体 (C-4) 参考文献:

  1. 鉴定 Nogo 轴突再生抑制因子为网状结构蛋白  |  GrandPré, T., et al. 2000. Nature. 403: 439-44. PMID: 10667797
  2. Nogo-A/Nogo-A受体在阿尔茨海默病中的作用。  |  Xu, YQ., et al. 2015. CNS Neurosci Ther. 21: 479-85. PMID: 25732725
  3. Nogo-A抗体用于进行性多发性硬化症。  |  Ineichen, BV., et al. 2017. CNS Drugs. 31: 187-198. PMID: 28105588
  4. RTN4/NoGo-受体与BAI粘附-GPCR的结合调节神经元发育。  |  Wang, J., et al. 2021. Cell. 184: 5869-5885.e25. PMID: 34758294
  5. RTN4/NoGo-受体与BAI粘附-GPCR的结合调节神经元发育。  |  Wang, J., et al. 2022. Cell. 185: 218. PMID: 34995515

订购信息

产品名称产品编号规格价格数量收藏夹

Nogo 抗体 (C-4)

sc-271878
200 µg/ml
RMB2377.00

Nogo (C-4): m-IgGκ BP-HRP 套装

sc-522088
200 µg Ab, 40 µg BP
RMB2662.00

Nogo 抗体 (C-4) AC

sc-271878 AC
500 µg/ml, 25% agarose
RMB3129.00

Nogo 抗体 (C-4) Alexa Fluor® 488

sc-271878 AF488
200 µg/ml
RMB2685.00

Nogo 抗体 (C-4) Alexa Fluor® 647

sc-271878 AF647
200 µg/ml
RMB2685.00

Nogo 抗体 (C-4) FITC

sc-271878 FITC
200 µg/ml
RMB2482.00

Nogo 抗体 (C-4) HRP

sc-271878 HRP
200 µg/ml
RMB2377.00

Nogo (C-4) 中和勝肽

sc-271878 P
100 µg/0.5 ml
RMB511.00

Nogo 抗体 (C-4) PE

sc-271878 PE
200 µg/ml
RMB2580.00

What region of Nogo-A, B, and C does this antibody recognize? I have a KO mouse with a flxed region of Exon 4/5 ( first two common exons between all Nogo proteins). I am still seeing Nogo expression in cells where Nogo should be KO. Confirmed KO via PCR 

Asked by: elixir_w
Thank you for your question. Nogo Antibody (C-4): sc-271878 is specific for an epitope mapping between amino acids 18-41 at the N-terminus of Nogo of human origin.
Answered by: Tech Support Europe
Date published: 2022-11-30

The sample blot shows a protein of about 55 kDa, corresponding to the Nogo B isoform, but no band around 125 kDa, the molecular weight of the Nogo A isoform. Is this antibody preference or differential expression of the isoforms in HEK cells?

Asked by: shoran
Thank you for your question. The Western blot data shown on our website is representative of what you can expect when using this antibody. We haven't tested for differential isoform expression in HEK cells. There is Western blot data in Neuro-2A and EOC20 whole cell lysates showing a signal detected around 220 kDa, the expected observed molecular weight for Nogo A.
Answered by: Technical Support
Date published: 2019-02-25

What is the Molecular Weight of the Nogo Antibody (C-4)?

Asked by: asj64
We do not determine the exact molecular weight for each individual antibody. However, the expected molecular weight detected by this Nogo Antibody (C-4): sc-271878 can be found on our product datasheet. A link is provided below. https://datasheets.scbt.com/sc-271878.pdf
Answered by: Technical Support
Date published: 2018-01-10

What application is the blocking peptide sc-271878 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from very strong band!I bought this antibody two weeks for WB, then I got a strong band.
Date published: 2019-07-16
Rated 5 out of 5 by from Great Antibody!I tested 30-32 antibodies from Santa Cruz. I used the Nogo Antibody (C-4) to detect RTN4 from mouse brain lysates. I detected a single band in WB (1:200).
Date published: 2018-07-05
Rated 5 out of 5 by from Excellent for NogoA & B, but not for NoGo CIt's an excellent antibody for detection of NogoA and NogoB in western blot and IF. I used 1:60 dilution of this antibody in IF, for staining Nogo A & B in PFA fixed HeLa cells. I used 1:200 dilution in western blot for detecting NogoA & B in HeLa cell lysate. There was a strong band for NogoB and a faint band for NogoA, but no band for NogoC.
Date published: 2018-02-23
Rated 5 out of 5 by from RecommendedNogo Antibody are involved in some advantages, such as high specificity, sensitivity, convenience, low cost and short cycle.
Date published: 2017-10-18
Rated 5 out of 5 by from very good antibodyI have tried twoNogo A antibodies of the other companies for several times, but the results are disappointed. This one is good, you can easily get strong and clear bands with this antibody.
Date published: 2017-09-27
Rated 5 out of 5 by from Great antibodyWorked well for IF staining of retinal cross sections from mouse.
Date published: 2017-09-18
Rated 3 out of 5 by from Not usable on J774A.1 cellsProduces only very dim diffuse staining of J774A.1 cells. On the other hand, it was a free sample, so no harm done.
Date published: 2017-05-11
Rated 5 out of 5 by from Produced publishable WB data in human and ratThis Nogo monoclonal antibody was referenced in the literature for the use of WB on human and mouse samples
Date published: 2017-02-09
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Nogo 抗体 (C-4) is rated 4.8 out of 5 by 11.
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