Date published: 2025-10-24

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Nodal 抗体 (WS65): sc-81953

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说明书
  • Nodal抗体(WS65)是小鼠单克隆IgG1 κ, 在7篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组Nodal
  • 推荐用于 mouse, rat 和 human 来源的Nodal WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Nodal Antibody (WS65) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与Nodal Antibody (WS65) 打包提供(请参阅下面的订购信息)。

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関連項目

Nodal抗体(WS65)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的Nodal。抗节点抗体(WS65)以非结合形式提供,可满足各种实验需求。节点是转化生长因子β(TGFβ)超家族的一员,在胚胎发育中起着至关重要的作用,特别是在胚层形成期间。节点在原始条纹中表达,外胚层细胞在此处脱层并分化成中胚层,这凸显了其在早期发育过程中的重要性。此外,Nodal还参与调节发育中胚胎的左右不对称性,影响心脏回旋和胚胎整体旋转的方向。了解Nodal的功能至关重要,因为失调会导致发育障碍和先天性异常。Nodal单克隆抗体(WS65)具有广泛的物种反应性和多种应用,是研究发育生物学和相关领域的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nodal 抗体 (WS65) 参考文献:

  1. 房室结折返性心动过速环路:建议。  |  Katritsis, DG. and Becker, A. 2007. Heart Rhythm. 4: 1354-60. PMID: 17905343
  2. 小鼠子宫中的结点表达受胚胎调控并与植入相关。  |  Park, CB. and Dufort, D. 2011. Biol Reprod. 84: 1103-10. PMID: 21270430
  3. 转化生长因子-β 家族。  |  Massagué, J. 1990. Annu Rev Cell Biol. 6: 597-641. PMID: 2177343
  4. Nodal 可促进 M2 样巨噬细胞的生成,并下调 IL-12 的表达。  |  Wang, XF., et al. 2014. Eur J Immunol. 44: 173-83. PMID: 24114072
  5. 2:1房室传导阻滞伴房室结折返性心动过速。  |  Larroussi, L. and Badhwar, N. 2016. Card Electrophysiol Clin. 8: 51-5. PMID: 26920169
  6. 多种乙型转化生长因子及其受体。  |  Massagué, J., et al. 1987. J Cell Physiol Suppl. Suppl 5: 43-7. PMID: 2890647
  7. 室-希氏和室-结旁路临床表现。  |  Chung, R., et al. 2019. Heart Rhythm. 16: 369-377. PMID: 30103070
  8. 原始条纹  |  Bellairs, R. 1986. Anat Embryol (Berl). 174: 1-14. PMID: 3518538
  9. 超高密度成像用于安全有效地进行典型房室结折返性心动过速消融。  |  Sakamoto, Y. and Osanai, H. 2023. Nagoya J Med Sci. 85: 844-851. PMID: 38155630
  10. Nodal 是一种新型 TGF-beta 类基因,在小鼠结节的胃形成过程中表达。  |  Zhou, X., et al. 1993. Nature. 361: 543-7. PMID: 8429908
  11. 不对称结节表达与胚胎转向之间的关系  |  Collignon, J., et al. 1996. Nature. 381: 155-8. PMID: 8610012
  12. 一种新的 TGF-beta 超家族成员对小鼠骨骼肌质量的调节。  |  McPherron, AC., et al. 1997. Nature. 387: 83-90. PMID: 9139826

订购信息

产品名称产品编号规格价格数量收藏夹

Nodal 抗体 (WS65)

sc-81953
100 µg/ml
RMB2504.00

Nodal (WS65): m-IgG Fc BP-HRP 套装

sc-539674
100 µg Ab; 10 µg BP
RMB2662.00

Nodal (WS65): m-IgG1 BP-HRP 套装

sc-541653
100 µg Ab; 20 µg BP
RMB2662.00

Can sc-81953: Nodal (WS65) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, sc-81953: Nodal (WS65) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from WS65 is good a antibody, but should give accurateWS65 is good a antibody, but should give accurate binding peptide sequence. I have used WS65 on Western blot to detect mature rhNodal for 15 ng (cat#: 3218-ND-025/CF, R&D), 13 kDa. If disclosing an accurate binding peptide sequence It will be more useful and helpful for researchers. I can use it on other project with the sequence.
Date published: 2021-04-26
Rated 5 out of 5 by from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-20
Rated 4 out of 5 by from Produced robust immunofluorescence cytoplasmicProduced robust immunofluorescence cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-05-18
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Nodal 抗体 (WS65) is rated 4.3 out of 5 by 4.
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