Date published: 2025-9-7

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NNMT 抗体 (G-4): sc-376048

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说明书
  • NNMT 抗体 G-4 是小鼠单克隆 IgG2a κ,NNMT抗体, 在12篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列191-258位于human物种的NNMT的C-terminal附近
  • NNMT 抗体 (G-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 NNMT
  • 抗NNMT抗体(G-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-376048 X, 200 µg/0.1 ml)
  • 关于如何获取NNMT (G-4): sc-376048免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是NNMT Antibody (G-4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与NNMT Antibody (G-4) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

NNMT抗体(G-4)是一种IgG2a κ小鼠单克隆NNMT抗体(也称为NNMT抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的NNMT蛋白。NNMT抗体(G-4)既可以是非偶联的抗NNMT抗体形式,也可以是多种偶联形式的抗NNMT抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。烟酰胺N-甲基转移酶(NNMT)催化烟酰胺和其他吡啶的N-甲基化。人体肝脏中的NNMT活性呈双峰频率分布,表明其酶活性可能通过遗传多态性进行调控,这可能对药物和异生素毒性的个体差异产生功能性影响。编码人类NNMT的基因长度约为16.5 kb,由三个外显子和两个内含子组成,定位于11q23.2。从人肝中分离出的NNMT被确定为969个核苷酸长度,具有792个核苷酸的开放阅读框,编码264个氨基酸的蛋白质。NNMT基因被认为是西班牙家庭中血浆同型半胱氨酸水平的重要遗传决定因素,因为它编码一种参与同型半胱氨酸合成的酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NNMT 抗体 (G-4) 参考文献:

  1. 人类烟酰胺 N-甲基转移酶药物遗传学:基因序列分析和启动子特征。  |  Yan, L., et al. 1999. Pharmacogenetics. 9: 307-16. PMID: 10471062
  2. 帕金森患者大脑中烟酰胺 N-甲基转移酶(E.C. 2.1.1.1)的表达。  |  Parsons, RB., et al. 2002. J Neuropathol Exp Neurol. 61: 111-24. PMID: 11853016
  3. 特发性帕金森病患者体内烟酰胺 N-甲基转移酶的高表达。  |  Parsons, RB., et al. 2003. Neurosci Lett. 342: 13-6. PMID: 12727306
  4. 人甲状腺乳头状癌细胞中烟酰胺 N-甲基转移酶的表达增强。  |  Xu, J., et al. 2003. J Clin Endocrinol Metab. 88: 4990-6. PMID: 14557485
  5. 肝细胞核因子-1β对人乳头状甲状腺癌细胞中烟酰胺 N-甲基转移酶基因启动子的激活作用  |  Xu, J., et al. 2005. Mol Endocrinol. 19: 527-39. PMID: 15486044
  6. 自体毒性, 甲基化和预防帕金森病之路。  |  Williams, AC. and Ramsden, DB. 2005. J Clin Neurosci. 12: 6-11. PMID: 15639403
  7. 帕金森病:第一种由自身中毒引起的常见神经系统疾病?  |  Williams, AC., et al. 2005. QJM. 98: 215-26. PMID: 15728403
  8. 一项全基因组探索表明,11q23 号染色体上的一个新候选基因是血浆同型半胱氨酸水平的主要决定因素:GAIT 项目的结果。  |  Souto, JC., et al. 2005. Am J Hum Genet. 76: 925-33. PMID: 15849667
  9. 烟酰胺稳态:发育和退行性疾病的关键异生物途径。  |  Williams, AC. and Ramsden, DB. 2005. Med Hypotheses. 65: 353-62. PMID: 15922112
  10. 鉴定烟酰胺 N-甲基转移酶作为结直肠癌的新型血清肿瘤标记物。  |  Roessler, M., et al. 2005. Clin Cancer Res. 11: 6550-7. PMID: 16166432
  11. 组蛋白去乙酰化酶抑制剂去西肽能抑制人乳头状甲状腺癌细胞中烟酰胺 N-甲基转移酶和肝细胞核因子-1beta基因的表达。  |  Xu, J. and Hershman, JM. 2006. Thyroid. 16: 151-60. PMID: 16676400
  12. 小鼠烟酰胺 N-甲基转移酶基因:分子克隆, 结构特征和染色体定位。  |  Yan, L., et al. 1998. DNA Cell Biol. 17: 659-67. PMID: 9726248

订购信息

产品名称产品编号规格价格数量收藏夹

NNMT 抗体 (G-4)

sc-376048
200 µg/ml
RMB2377.00

NNMT (G-4): m-IgGκ BP-HRP 套装

sc-552033
200 µg Ab; 40 µg BP
RMB2662.00

NNMT 抗体 (G-4) X

sc-376048 X
200 µg/0.1 ml
RMB2377.00

NNMT 抗体 (G-4) AC

sc-376048 AC
500 µg/ml, 25% agarose
RMB3129.00

NNMT 抗体 (G-4) HRP

sc-376048 HRP
200 µg/ml
RMB2377.00

NNMT 抗体 (G-4) FITC

sc-376048 FITC
200 µg/ml
RMB2482.00

NNMT 抗体 (G-4) PE

sc-376048 PE
200 µg/ml
RMB2580.00

NNMT 抗体 (G-4) Alexa Fluor® 488

sc-376048 AF488
200 µg/ml
RMB2685.00

NNMT 抗体 (G-4) Alexa Fluor® 546

sc-376048 AF546
200 µg/ml
RMB2685.00

NNMT 抗体 (G-4) Alexa Fluor® 594

sc-376048 AF594
200 µg/ml
RMB2685.00

NNMT 抗体 (G-4) Alexa Fluor® 647

sc-376048 AF647
200 µg/ml
RMB2685.00

NNMT 抗体 (G-4) Alexa Fluor® 680

sc-376048 AF680
200 µg/ml
RMB2685.00

NNMT 抗体 (G-4) Alexa Fluor® 790

sc-376048 AF790
200 µg/ml
RMB2685.00

I need to use NNMT (G-4): sc-376048 monoclonal antibody in mouse samples by Western blot. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2022-08-10
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Rated 5 out of 5 by from Funziona molto bene in WBUtilizzato per WB in A549 e H1944, alla concentrazione di 1:200 in Latte 5% in PBS restitituisce una banda singola intorno ai 30 kDA. La banda scompare dopo silenziamento del gene NNMT in A549.
Date published: 2025-01-16
Rated 4 out of 5 by from WorksWe used for WB 1:500 and worked well with low background
Date published: 2021-10-29
Rated 5 out of 5 by from Good signalI used this antibody for Western blot at 1:100 in 5% milk and it gave me a good signal at around 28kDa.
Date published: 2017-10-09
Rated 5 out of 5 by from Good for WBI bought this mono-antibody months ago for WB, results show that the band is unique.
Date published: 2017-09-27
Rated 5 out of 5 by from Beautiful results in WB!NNMT (G-4) monoclonal antibody gives clean and specific results in WB and IHC(P) applications.
Date published: 2017-01-19
Rated 5 out of 5 by from WBRight band size was detected in human derived samples, and almost there was no background
Date published: 2017-01-17
Rated 5 out of 5 by from Worked great for WB !Worked great for WB with my cell line! It worked with concentration at 1:1000.
Date published: 2016-12-01
Rated 5 out of 5 by from WB and IHC publishable data with human nasopharyngealWB and IHC publishable data with human nasopharyngeal carcinoma - SCBT Publication Review
Date published: 2015-01-22
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NNMT 抗体 (G-4) is rated 4.9 out of 5 by 9.
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