Date published: 2025-9-8
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NLRC5 Antibody (B-10) - Western Blotting - Image 356250
NLRC5 抗体 (B-10): sc-515668. TK-1 (A), I-11. 15 (B), SP2/0 (C), AMJ2-C8 (D) 和 MH-S (E) 全细胞裂解液中 NLRC5 表达的 Western 印迹分析.
NLRC5 Antibody (B-10) - Western Blotting - Image 356250
NLRC5 抗体 (B-10): sc-515668. TK-1 (A), I-11. 15 (B), SP2/0 (C), AMJ2-C8 (D) 和 MH-S (E) 全细胞裂解液中 NLRC5 表达的 Western 印迹分析.
NLRC5 Antibody (B-10) - Western Blotting - Image 360592
NLRC5 抗体 (B-10): sc-515668. RBL-1 (A), KARPAS-299 (B) 和 SU-DHL-1 (C) 全细胞裂解液中 NLRC5 表达的 Western 印迹分析.
NLRC5 Antibody (B-10) - Western Blotting - Image 383315
NLRC5 抗体 (B-10): sc-515668. Jurkat (A), K-562 (B), HEL 92. 1. 7 (C) 和 CCRF-CEM (D) 全细胞裂解液中 NLRC5 表达的 Western 印迹分析.
NLRC5 抗体 (B-10): sc-515668. TK-1 (A), I-11. 15 (B), SP2/0 (C), AMJ2-C8 (D) 和 MH-S (E) 全细胞裂解液中 NLRC5 表达的 Western 印迹分析.

NLRC5 抗体 (B-10): sc-515668

4.7(3)
寫評論提問

说明书
  • NLRC5 抗体 B-10 是小鼠单克隆 IgM κ，NLRC5抗体, 在11篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的的N-terminus附近的氨基酸41-64之间
  • 推荐用于 mouse, rat 和 human 来源的NLRC5 WB, IP, IF 和 ELISA检测
  • 关于如何获取NLRC5 (B-10): sc-515668的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是NLRC5 Antibody (B-10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与NLRC5 Antibody (B-10) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
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说明书与实验方案
研究信息

関連項目

NLRC5抗体（B-10）是一种小鼠单克隆IgM kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的NLRC5蛋白。抗NLRC5抗体（B-10）以非结合形式提供。NLRC5，也称为NOD4或NOD27，是一种关键的1866个氨基酸细胞质蛋白，在调节NF-κB和I/II型干扰素信号通路中发挥重要作用，对免疫反应和炎症至关重要。NLRC5包含一个NACHT结构域和26个富含亮氨酸的重复结构域（LRRs），这些结构域有助于蛋白质-蛋白质相互作用并促进调节功能。这些LRR形成疏水的α/β马蹄形折叠，对于识别和结合各种配体至关重要，从而影响先天免疫和抗病毒防御机制。NLRC5在包括大脑、肺、胸腺、心脏、脾脏和前列腺在内的多个组织中表达，并存在六个可替代剪接的同种型，这表明其在不同生物学环境中的功能作用是多种多样的。NLRC5基因位于人类第16号染色体上，这凸显了其在免疫反应相关遗传研究和潜在治疗靶点中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NLRC5 抗体 (B-10) 参考文献:

  1. 作为蛋白质识别图案的富亮氨酸重复序列。  |  Kobe, B. and Kajava, AV. 2001. Curr Opin Struct Biol. 11: 725-32. PMID: 11751054
  2. 家族性地中海热基因产物 pyrin 对 cryopyrin/Pypaf1 信号的调控。  |  Dowds, TA., et al. 2003. Biochem Biophys Res Commun. 302: 575-80. PMID: 12615073
  3. 与人类疾病相关的蛋白质中富含亮氨酸重复变体的结构分析。  |  Matsushima, N., et al. 2005. Cell Mol Life Sci. 62: 2771-91. PMID: 16231091
  4. 人角膜上皮细胞中 Nod 样受体及其功能伙伴的转录受制和紫外线-B 调节。  |  Benko, S., et al. 2008. Mol Vis. 14: 1575-83. PMID: 18769647
  5. 核苷酸结合寡聚化结构域样受体 NLRC5 参与了 IFN 依赖性抗病毒免疫反应。  |  Kuenzel, S., et al. 2010. J Immunol. 184: 1990-2000. PMID: 20061403
  6. NLRC5 负向调节 NF-kappaB 和 I 型干扰素信号通路。  |  Cui, J., et al. 2010. Cell. 141: 483-96. PMID: 20434986
  7. NLRC5：免疫和炎症反应中 NLRs 的范例。  |  Wu, Y., et al. 2019. Cancer Lett. 451: 92-99. PMID: 30867141
  8. PRMT5 对 cGAS/STING 和 NLRC5 通路的控制决定了黑色素瘤对抗肿瘤免疫的反应。  |  Kim, H., et al. 2020. Sci Transl Med. 12: PMID: 32641491
  9. NLRC5：中枢神经系统疾病的潜在靶点。  |  Zhang, L., et al. 2021. Front Immunol. 12: 704989. PMID: 34220868
  10. NLRC5 可感知 NAD+ 的耗竭，形成 PANoptosome 并驱动 PANoptosis 和炎症。  |  Sundaram, B., et al. 2024. Cell. 187: 4061-4077.e17. PMID: 38878777
  11. 富含亮氨酸重复序列的蛋白质。  |  Kobe, B. and Deisenhofer, J. 1995. Curr Opin Struct Biol. 5: 409-16. PMID: 7583641
  12. 富含亮氨酸的重复序列：一种多功能的结合图案。  |  Kobe, B. and Deisenhofer, J. 1994. Trends Biochem Sci. 19: 415-21. PMID: 7817399

订购信息

产品名称产品编号规格价格数量收藏夹

NLRC5 抗体 (B-10)

sc-515668
200 µg/ml
RMB2377.00

NLRC5 (B-10): m-IgGκ BP-HRP 套装

sc-525154
200 µg Ab, 40 µg BP
RMB2662.00

这支抗体的规格是200微克/ml，那么它有多少微升呢

Asked by: wufan
感谢您的提问。体积是1000ul. Thank you for your question. The volume is 1000ul. Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Sen Li
Date published: 2025-08-08

Is the NLRC5 (B-10): sc-515668 mouse monoclonal antibody cross-reactive to human NLRC5? In other words, in the figure with the WB shown above are the cell lines transfected with mouse Nlrc5 or this is the endogenous signal?

Asked by: Hristo
Thank you for your question. Yes, this antibody is reactive with mouse, rat and human NLRC5. The Western blot data shown is endogenous expression in the cell lines tested.
Answered by: Technical Support
Date published: 2019-02-23

Hi,I am a student working with NLRC5 Antibody (sc-515668). The bands I got from Western Blotting are different from the datasheet. I got 3 bands, and they are about: 150kDa, 100kDa and 78kDa. Could you help me with this problem? Thank you very much!

Asked by: Yanan 123
Thank you for your question. According to Uniprot, NLRC5 has two predicted isoforms at 212 and 154 kDa. The observed molecular weight of the protein may be different than the predicted molecular weight due to post-translation modifications or alterations to the endogenous protein. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-02-20

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if NLRC5 (B-10): sc-515668 mouse monoclonal antibody will work?

Asked by: cjMara
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from A working productThe product works well in mouse primary T cells and other immune cells.
Date published: 2024-09-26
Rated 5 out of 5 by from Good choice for Western BlotAntibody works consistently in TK-1 and Jurkat whole cell lysates. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from good for WBAb was diluted at 1:1000. Bands are clear, good for WB.
Date published: 2017-07-20
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NLRC5 抗体 (B-10) is rated 4.7 out of 5 by 3.
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