Date published: 2025-9-8

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Ninein 抗体 (F-5): sc-376420

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说明书
  • Ninein 抗体 F-5 是小鼠单克隆 IgG1 κ,Ninein抗体, 在11篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列289-476位于human物种的Ninein的N-terminus附近
  • Ninein 抗体 (F-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Ninein
  • 抗Ninein抗体(F-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ninein (F-5): sc-376420免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Ninein Antibody (F-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Ninein Antibody (F-5) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Ninein抗体(F-5)是一种IgG1 κ小鼠单克隆Ninein抗体(也称为NIN抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Ninein蛋白。Ninein抗体(F-5)既可作为非偶联形式的抗Ninein抗体提供,也可作为多种偶联形式的抗Ninein抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ninein是一种中心体蛋白,对于上皮细胞中微管负端的定位和锚定是必要的。该蛋白可能是一种中心体成熟因子,可能在微管成核中发挥作用。Ninein的过表达并不改变微管的成核或延长,而是抑制其释放。Ninein通过其C端结构域与GSK3B(GSK3-β)结合,并与C14orf166相互作用;后者被认为可以阻止GSK-3β对Ninein的磷酸化。Ninein是中心体核心的一个组成部分,在中心体内以管状构象排列,其开放端和封闭端都包含在中心体内。它表现出普遍的表达,并在心脏和骨骼肌组织中显示主要表达。从Asn 1611到Pro 1693的卷曲螺旋区域对于将Ninein定位到中心体是必要的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ninein 抗体 (F-5) 参考文献:

  1. 预测人类不明基因的编码序列。XVIII.100 个新的大脑 cDNA 克隆的完整序列,它们在体外编码大型蛋白质。  |  Nagase, T., et al. 2000. DNA Res. 7: 273-81. PMID: 10997877
  2. 克隆并鉴定与糖原合酶激酶 3beta 相互作用的新型人类 ninein 蛋白。  |  Hong, YR., et al. 2000. Biochim Biophys Acta. 1492: 513-6. PMID: 11004522
  3. CEP110 和 ninein 位于中心体的一个特定区域,与中心体成熟有关。  |  Ou, YY., et al. 2002. J Cell Sci. 115: 1825-35. PMID: 11956314
  4. 人类 ninein 蛋白的分子特征:中心体靶向和调节细胞周期信号所需的两个不同亚域。  |  Chen, CH., et al. 2003. Biochem Biophys Res Commun. 308: 975-83. PMID: 12927815
  5. 人类 ninein 是一种被 CREST 患者血清识别的中心体自身抗原,在微管成核过程中发挥调节作用。  |  Stillwell, EE., et al. 2004. Cell Cycle. 3: 923-30. PMID: 15190203
  6. 微管相关蛋白 ninein 在发育中神经元中的分布。  |  Baird, DH., et al. 2004. Neuropharmacology. 47: 677-83. PMID: 15458839
  7. 微管的成核和在中心体的锚定是两个独立的过程,通过九连蛋白的功能联系在一起。  |  Delgehyr, N., et al. 2005. J Cell Sci. 118: 1565-75. PMID: 15784680
  8. 表皮的发育需要 ninein 进行纺锤体定向和皮层微管组织。  |  Lecland, N., et al. 2019. Life Sci Alliance. 2: PMID: 30923192
  9. 九因子在巨噬细胞吞噬过程中促进F-肌动蛋白杯的形成和内吞泡向内运动。  |  Omer, S., et al. 2024. Mol Biol Cell. 35: ar26. PMID: 38117588
  10. 九因子的缺失会干扰破骨细胞的形成并导致过早骨化。  |  Gilbert, T., et al. 2024. Elife. 13: PMID: 38836552
  11. 人类 ninein 基因的基因组结构和分子特征。  |  Hong, YR., et al. 2000. Biochem Biophys Res Commun. 279: 989-95. PMID: 11162463
  12. 中心粒蛋白hNinein的SUMO-1修饰促进了hNinein的核定位。  |  Cheng, TS., et al. 2006. Life Sci. 78: 1114-20. PMID: 16154161

订购信息

产品名称产品编号规格价格数量收藏夹

Ninein 抗体 (F-5)

sc-376420
200 µg/ml
RMB2377.00

Ninein (F-5): m-IgGκ BP-HRP 套装

sc-522951
200 µg Ab, 40 µg BP
RMB2662.00

Ninein 抗体 (F-5) AC

sc-376420 AC
500 µg/ml, 25% agarose
RMB3129.00

Ninein 抗体 (F-5) HRP

sc-376420 HRP
200 µg/ml
RMB2377.00

Ninein 抗体 (F-5) FITC

sc-376420 FITC
200 µg/ml
RMB2482.00

Ninein 抗体 (F-5) PE

sc-376420 PE
200 µg/ml
RMB2580.00

Ninein 抗体 (F-5) Alexa Fluor® 488

sc-376420 AF488
200 µg/ml
RMB2685.00

Ninein 抗体 (F-5) Alexa Fluor® 546

sc-376420 AF546
200 µg/ml
RMB2685.00

Ninein 抗体 (F-5) Alexa Fluor® 594

sc-376420 AF594
200 µg/ml
RMB2685.00

Ninein 抗体 (F-5) Alexa Fluor® 647

sc-376420 AF647
200 µg/ml
RMB2685.00

Ninein 抗体 (F-5) Alexa Fluor® 680

sc-376420 AF680
200 µg/ml
RMB2685.00

Ninein 抗体 (F-5) Alexa Fluor® 790

sc-376420 AF790
200 µg/ml
RMB2685.00

The ab works great for IF but a have prominent band in mouse lysate just above 75kDa and not the expected 250.

Asked by: somer
Please contact Technical Support
Answered by: Tech Sales
Date published: 2021-04-07

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-376420: Ninein (F-5) mouse monoclonal antibody will work?

Asked by: cjMara
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2019-03-16
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Rated 1 out of 5 by from Did not work for IF in human RPE-hTERT cellsThis antibody did not work (no centrosomal staining) for IF in 4% PFA fixed RPE-hTERT cells.
Date published: 2018-02-28
Rated 5 out of 5 by from Works in ImmunofluorescenceI tested this antibody in IF, MetOH fixation, 1/250 dilution in RPE-1 cells.
Date published: 2018-02-15
Rated 3 out of 5 by from Not Very Wellnot work well in mouse testis, the results I got were not so clear ,and the concentrition need more tests
Date published: 2017-04-20
Rated 5 out of 5 by from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-27
Rated 5 out of 5 by from Exellent antibodyWorks well on IF in RPE-1 cells; can see the 3+1 staining pattern indicative of subdistal appendages and proximal centriolar ends.
Date published: 2016-12-07
Rated 5 out of 5 by from Produced positive Western blot data of NineinProduced positive Western blot data of Ninein expression in rat skeletal muscle tissue extract. -SCBT QC
Date published: 2013-11-25
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Ninein 抗体 (F-5) is rated 4.0 out of 5 by 6.
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