Date published: 2025-9-6
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RELA/NFκB p65 Antibody (F-6) - Western Blotting - Image 390728
NFκB p65 抗体 (F-6) Alexa Fluor® 594: sc-8008 AF594. THP-1 (A), K-562 (B), MOLT-4 (C), Jurkat (D), T24 (E) 和 HUV-EC-C (F) 全细胞裂解液中 NFκB p65 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
NFκB p65 抗体 (F-6) Alexa Fluor® 594: sc-8008 AF594. THP-1 (A), K-562 (B), MOLT-4 (C), Jurkat (D), T24 (E) 和 HUV-EC-C (F) 全细胞裂解液中 NFκB p65 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

RELA/NFκB p65 抗体 (F-6): sc-8008

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说明书
  • RELA/NFκB p65 抗体 F-6 是小鼠单克隆 IgG1 κ，RELA/NFκB p65抗体, 在2,028篇文献中引用，规格为200 µg/ml
  • 免疫human来源NFκB p65位于N-terminus 的1-286氨基酸
  • RELA/NFκB p65 抗体 (F-6) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 NFκB p65
  • 抗RELA/NFκB p65抗体（F-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联生物素 用于WB, IHC(P) 以及 ELISA;偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8008 X, 200 µg/0.1 ml)
  • 关于如何获取RELA/NFκB p65 (F-6): sc-8008的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是RELA/NFκB p65 Antibody (F-6) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与RELA/NFκB p65 Antibody (F-6) 搭配使用（请参阅下面的订购信息）。

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说明书与实验方案
研究信息

関連項目

额外NF κ B抗体，包括NF κ B p50, NF κ B p52和NF κ B p65

NFκB p65抗体（F-6）是一种小鼠单克隆IgG1 kappa轻链抗体，针对人源NFκB p65蛋白N端的1-286个氨基酸。NFκB p65 单克隆抗体（F-6）可与小鼠、大鼠和人类样本发生反应，因此 NFκB p65 (F-6) 抗体非常适合用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）等应用。NFκB p65，也称为RelA，是NFκB转录因子复合物的重要组成部分，可调节与炎症、免疫反应、细胞存活和增殖相关的基因。NFκB p65的激活始于IκB蛋白的降解，在静止状态下，IκB蛋白会将NFκB隔离在细胞质中。一旦IκB被降解，NFκB p65就会被释放并迅速转移到细胞核中，这一过程由核定位信号（NLS）促进。这种转移使NFκB p65能够进入并结合目标基因启动子区域中的特定κB增强子序列，从而启动转录。NFκB p65在细胞质和细胞核之间的动态移动受到严格调控，对于环境和细胞信号的快速和特定转录反应至关重要。研究这种转位对于理解NFκB活性的失调如何导致炎症性疾病和癌症至关重要。NFκB p65单克隆抗体（F-6）有非结合和结合两种形式，包括HRP、PE、FITC和Alexa Fluor®结合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RELA/NFκB p65 抗体 (F-6) 参考文献:

  1. NF-kappa B 前体 p105 和原癌基因 bcl-3 的杏仁蛋白重复结构域是 NF-kappa B DNA 结合的特异性抑制剂。  |  Hatada, EN., et al. 1992. Proc Natl Acad Sci U S A. 89: 2489-93. PMID: 1532257
  2. NF-kappa B 亚基的不同组合决定了转录激活的特异性。  |  Perkins, ND., et al. 1992. Proc Natl Acad Sci U S A. 89: 1529-33. PMID: 1542644
  3. 人类 NF-kappa B 的 65 kDa 亚基是一种强效转录激活剂，也是 v-Rel 介导的抑制作用的靶标。  |  Ballard, DW., et al. 1992. Proc Natl Acad Sci U S A. 89: 1875-9. PMID: 1542686
  4. 牛 NFkappaB p65 亚基的克隆, 表达和表征。  |  Doleschall, M., et al. 2007. Dev Comp Immunol. 31: 945-61. PMID: 17306370
  5. 在糖尿病肾病大鼠模型中，高血压会加重氧化应激导致的肾小球功能障碍。  |  Tomohiro, T., et al. 2007. Life Sci. 80: 1364-72. PMID: 17331548
  6. 系统性红斑狼疮患者体内的糖皮质激素受体, 核因子卡巴B, 激活蛋白-1和C-jun N-末端激酶。  |  Oikonomidou, O., et al. 2006. Neuroimmunomodulation. 13: 194-204. PMID: 17347585
  7. 显性阴性激活蛋白 1（TAM67）以环氧化酶-2 和骨化蛋白为靶标，在这种情况下，它能特异性地抑制肿瘤的发生。  |  Matthews, CP., et al. 2007. Cancer Res. 67: 2430-8. PMID: 17363560
  8. 克隆与 p65 协同刺激 HIV 转录的 NF-kappa B 亚基。  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
  9. 克隆人类核因子卡巴 B 的 DNA 结合亚基：其 mRNA 水平受植物醇酯或肿瘤坏死因子 alpha 的强烈调控。  |  Meyer, R., et al. 1991. Proc Natl Acad Sci U S A. 88: 966-70. PMID: 1992489
  10. CircRNF10-DHX15相互作用通过拮抗DHX15-NF-κB p65的正反馈环抑制乳腺癌进展。  |  Zheng, W., et al. 2023. Cell Mol Biol Lett. 28: 34. PMID: 37101128
  11. 丹参酮IIA通过TLR4/NF-κB p65通路在急性心肌梗死后对心肌细胞凋亡具有抑制作用。  |  Chai, R., et al. 2023. Front Cell Dev Biol. 11: 1252942. PMID: 37766966
  12. 通过招募 SOCS1 促进 p65 的泛素化降解作用，假狂犬病病毒 DNA 聚合酶进程因子 UL42 抑制 NF-κB 信号传导。  |  Bo, Z., et al. 2023. Vet Microbiol. 287: 109896. PMID: 37931575

订购信息

产品名称产品编号规格价格数量收藏夹

RELA/NFκB p65 抗体 (F-6)

sc-8008
200 µg/ml
RMB2377.00

RELA/NFκB p65 (F-6): m-IgG Fc BP-HRP 套装

sc-525447
200 µg Ab; 10 µg BP
RMB2662.00

RELA/NFκB p65 抗体 (F-6) X

sc-8008 X
200 µg/0.1 ml
RMB2377.00

RELA/NFκB p65 抗体 (F-6) AC

sc-8008 AC
500 µg/ml, 25% agarose
RMB3129.00

RELA/NFκB p65 抗体 (F-6) HRP

sc-8008 HRP
200 µg/ml
RMB2377.00

RELA/NFκB p65 抗体 (F-6) FITC

sc-8008 FITC
200 µg/ml
RMB2482.00

RELA/NFκB p65 抗体 (F-6) PE

sc-8008 PE
200 µg/ml
RMB2580.00

RELA/NFκB p65 抗体 (F-6) Alexa Fluor® 488

sc-8008 AF488
200 µg/ml
RMB2685.00

RELA/NFκB p65 抗体 (F-6) Alexa Fluor® 546

sc-8008 AF546
200 µg/ml
RMB2685.00

RELA/NFκB p65 抗体 (F-6) Alexa Fluor® 594

sc-8008 AF594
200 µg/ml
RMB2685.00

RELA/NFκB p65 抗体 (F-6) Alexa Fluor® 647

sc-8008 AF647
200 µg/ml
RMB2685.00

RELA/NFκB p65 抗体 (F-6) Alexa Fluor® 680

sc-8008 AF680
200 µg/ml
RMB2685.00

RELA/NFκB p65 抗体 (F-6) Alexa Fluor® 790

sc-8008 AF790
200 µg/ml
RMB2685.00

RELA/NFκB p65 抗体 (F-6) B

sc-8008 B
200 µg/ml
RMB2407.00

RELA/NFκB p65 抗体 (F-6) TRITC

sc-8008 TRITC
200 µg/ml
RMB2407.00

RELA/NFκB p65 抗体 (F-6) Alexa Fluor® 405

sc-8008 AF405
200 µg/ml
RMB2685.00

Other than sc-516214 Blocking reagent, is this antibody compatible with blocking buffer 5% BSA TBST or 5% nonfat milk TBST for Western Blot application?

Asked by: ErnestY
Thank you for your question. We recommend 5% nonfat milk TBST . https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2025-07-28

I used sc-8008 on mouse skin, normal and imiquimod treated. Whereas the staining on the normal epidermis was intense and cytoplasmic in the basal layer, it almost disappeared on the inflammatory IMQ-treated skin epidermis. Did somebody experience the same

Asked by: MS31
Thank You for your question . Please contact our distributor in Europe by emailing [email protected].
Answered by: BlakeJ
Date published: 2022-11-14

Does anybody know if this product reacts with pig p65? it seems to be the most popular p65 antibody at SC.

Asked by: Aydin
Thank you for your question. Anti-NF kappa B p65 Antibody (F-6) is only recommended for detection of NFκB p65 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA. We do not have any internal data to support use of this antibody in porcine cells or tissues.
Answered by: Tech Support Europe
Date published: 2022-09-19

I need an antibody that will detect RELA in IHC, and I see that the NFκB p65 Antibody (C-20) sc-372 has been discontinued (it was referenced in a publication) and I was redirected here. Will this antibody (sc-8008) also detect RELA?

Asked by: Arianna1
Thank you for your question. The following antibodies are recommended for detecting NFκB p65 in IHC and IHC(P) applications: Anti-NFκB p65 Antibody (F-6) sc-8008 Anti-NFκB p65 Antibody (A-12) sc-514451 Anti-NFκB p65 Antibody (G-8) sc-398442
Answered by: Tech Support Europe
Date published: 2022-02-04

Buen día, saben por qué el anticuerpo NFκB p65 Anticuerpo (F-6), detecta dos bandas? y lo mismo ocurre con el anticuerpo para detectar p65 fosforilada. Gracias

Asked by: Normi
La proteína diana de este anticuerpo está sujeta a diversas modificaciones postraduccionales. Estos contribuyen al peso molecular observado de la proteína en transferencias Western.
Answered by: Tech Support Europe
Date published: 2023-11-16

Is this AB compatible with m-IgGκ BP-HRP: sc-516102 secondary AB?

Asked by: Sahin
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-06

Hi, I am testing this antibody to detect nuclear nf-kb in my cell lines. Could you suggest a protocol to isolate and only stain the nuclei with this antibody for flow cytometry? Thank you in advance for your help.

Asked by: rcengiz
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-07

We have this antibody and another that binds to phosphoNF-kB p65. We would like to know if this antibody (NFκB p65 Anticorpo (F-6): sc-8008) binds to total NF-kB (normal + phosphorilated) or only the normal version of the protein?

Asked by: Anonymous
Thank you for your question. NFκB p65 Antibody (F-6): sc-8008 recognizes both phosphorylated and non-phosphorylated NFκB p65.
Answered by: Tech Support Europe
Date published: 2024-08-21
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Rated 4 out of 5 by from good signalHeLa cells fixed with formaldehyde 3.7%, anti RELA/NFkB p65 1/100, 4°C O/N.
Date published: 2025-08-26
Rated 5 out of 5 by from GAPDH antibodyI have used the GAPDH antibody from Santa Cruz, and it has given me excellent results due to its stability and the ability to use it repeatedly.
Date published: 2025-04-23
Rated 5 out of 5 by from GoodGreat antibody for WB, works in mouse RAW 264.7 cells
Date published: 2025-03-18
Rated 5 out of 5 by from OttimoAbbiamo usato quest'anticorpo per analisi western blot con linee cellulari umane. Funziona bene con una diluzione 1:500
Date published: 2025-01-08
Rated 4 out of 5 by from GreatWe have used this antibody to publish high-quality WB data
Date published: 2024-10-16
Rated 5 out of 5 by from good useI applied for this sample product a month ago and it works very well
Date published: 2024-09-10
Rated 5 out of 5 by from Great features for western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-03
Rated 5 out of 5 by from Worked very well in human tissue by IHCWe had a great experience using this p65 Ab in IF studies using paraffin-embedded human tissues
Date published: 2024-05-30
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RELA/NFκB p65 抗体 (F-6) is rated 4.7 out of 5 by 174.
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