Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
NFκB p52/p100/NFKB2 Antibody (C-5) - Western Blotting - Image 1030
NFκB p52 (C-5): sc-7386. 对 HuT 78 全细胞裂解液中 NFκB p52 的表达进行 Western 印迹分析.
NFκB p52 (C-5): sc-7386. 对 HuT 78 全细胞裂解液中 NFκB p52 的表达进行 Western 印迹分析.

NFκB p52/p100/NFKB2 抗体 (C-5): sc-7386

4.8(18)
寫評論提問

说明书
  • NFκB p52/p100/NFKB2 抗体 C-5 是小鼠单克隆 IgG2a κ，NFκB p52/p100/NFKB2抗体, 在204篇文献中引用，规格为200 µg/ml
  • 免疫1-447氨基酸，对应于human来源NFκB p52全长
  • NFκB p52/p100/NFKB2 抗体 (C-5) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 NFκB p52 and p100
  • 抗NFκB p52/p100/NFKB2抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-7386 X, 200 µg/0.1 ml)
  • 关于如何获取NFκB p52/p100/NFKB2 (C-5): sc-7386的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是NFκB p52/p100/NFKB2 Antibody (C-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与NFκB p52/p100/NFKB2 Antibody (C-5) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外NF κ B抗体，包括NF κ B p50, NF κ B p52和NF κ B p65

NFκB p52抗体（C-5）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测小鼠、大鼠和人源的NFκB p52蛋白。NFκB p52 (C-5) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。NFκB p52在调节免疫反应、细胞存活和炎症方面发挥着关键作用，因此NFκB p52成为了解包括癌症和自身免疫性疾病在内的各种疾病的重要靶标。NFκB p52是通过其前体p105的加工产生的，并与其他NFκB亚基（如p65）形成异二聚体，以激活靶基因的转录。NFκB p52与p65相互作用，增强NFκB复合体的转录活性，这对于免疫和炎症反应相关基因的表达至关重要。了解NFκB p52的功能和相互作用对于阐明NFκB p52在病理生理过程中的作用以及开发靶向疗法至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NFκB p52/p100/NFKB2 抗体 (C-5) 参考文献:

  1. 原代乳腺上皮细胞在肿瘤坏死因子刺激下的生长不需要 NFkappaB1/p50：NFkappaB2/p52 和 RelB 的影响。  |  Zhang, J., et al. 2007. Endocrinology. 148: 268-78. PMID: 17008396
  2. 褐藻糖胶可阻止 B 细胞中 C epsilon 基因转录和 NFkappaB p52 转位以产生 IgE。  |  Oomizu, S., et al. 2006. Biochem Biophys Res Commun. 350: 501-7. PMID: 17027924
  3. RelB是一种能与p50-NF-kappa B相互作用的新型Rel家族转录激活因子。  |  Ryseck, RP., et al. 1992. Mol Cell Biol. 12: 674-84. PMID: 1732739
  4. 克隆与 p65 协同刺激 HIV 转录的 NF-kappa B 亚基。  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
  5. 克隆 NF-kappa B 的 p50 DNA 结合亚基：与 rel 和 dorsal 同源。  |  Ghosh, S., et al. 1990. Cell. 62: 1019-29. PMID: 2203532
  6. NF-kappa B, KBF1, 背鳍及相关事项。  |  Gilmore, TD. 1990. Cell. 62: 841-3. PMID: 2203533
  7. 克隆出一种有丝分裂原诱导基因，该基因编码一种卡巴 B DNA 结合蛋白，该蛋白与 rel 致癌基因和细胞周期图案具有同源性。  |  Bours, V., et al. 1990. Nature. 348: 76-80. PMID: 2234062
  8. 核因子κB2 p52蛋白通过IκB激酶epsilon依赖性诱导Sp1蛋白和白细胞介素15在抗病毒免疫中发挥作用。  |  Doyle, SL., et al. 2013. J Biol Chem. 288: 25066-25075. PMID: 23873932
  9. I kappaB抑制NF-kappaB需要活性NF-kappaB的65-kappaD亚基。  |  Baeuerle, PA. and Baltimore, D. 1989. Genes Dev. 3: 1689-98. PMID: 2691328
  10. 多种核因子与免疫球蛋白增强子序列相互作用。  |  Sen, R. and Baltimore, D. 1986. Cell. 46: 705-16. PMID: 3091258
  11. 人类RelB基因缺陷会削弱先天性和适应性免疫系统对感染的抵抗力。  |  Le Voyer, T., et al. 2024. Proc Natl Acad Sci U S A. 121: e2321794121. PMID: 39231201
  12. 高度表达的p100/p52（NFKB2）在人类乳腺癌细胞的细胞质中隔离其他NF-kappa B相关蛋白。  |  Dejardin, E., et al. 1995. Oncogene. 11: 1835-41. PMID: 7478612

订购信息

产品名称产品编号规格价格数量收藏夹

NFκB p52/p100/NFKB2 抗体 (C-5)

sc-7386
200 µg/ml
RMB2377.00

NFκB p52/p100/NFKB2 (C-5): m-IgG Fc BP-HRP 套装

sc-525441
200 µg Ab; 10 µg BP
RMB2662.00

NFκB p52/p100/NFKB2 (C-5): m-IgG2a BP-HRP 套装

sc-546256
200 µg Ab; 10 µg BP
RMB2662.00

NFκB p52/p100/NFKB2 抗体 (C-5) X

sc-7386 X
200 µg/0.1 ml
RMB2377.00

NFκB p52/p100/NFKB2 抗体 (C-5) AC

sc-7386 AC
500 µg/ml, 25% agarose
RMB3129.00

NFκB p52/p100/NFKB2 抗体 (C-5) HRP

sc-7386 HRP
200 µg/ml
RMB2377.00

NFκB p52/p100/NFKB2 抗体 (C-5) FITC

sc-7386 FITC
200 µg/ml
RMB2482.00

NFκB p52/p100/NFKB2 抗体 (C-5) PE

sc-7386 PE
200 µg/ml
RMB2580.00

NFκB p52/p100/NFKB2 抗体 (C-5) Alexa Fluor® 488

sc-7386 AF488
200 µg/ml
RMB2685.00

NFκB p52/p100/NFKB2 抗体 (C-5) Alexa Fluor® 546

sc-7386 AF546
200 µg/ml
RMB2685.00

NFκB p52/p100/NFKB2 抗体 (C-5) Alexa Fluor® 594

sc-7386 AF594
200 µg/ml
RMB2685.00

NFκB p52/p100/NFKB2 抗体 (C-5) Alexa Fluor® 647

sc-7386 AF647
200 µg/ml
RMB2685.00

NFκB p52/p100/NFKB2 抗体 (C-5) Alexa Fluor® 680

sc-7386 AF680
200 µg/ml
RMB2685.00

NFκB p52/p100/NFKB2 抗体 (C-5) Alexa Fluor® 790

sc-7386 AF790
200 µg/ml
RMB2685.00

NFκB p52/p100/NFKB2 抗体 (C-5) Alexa Fluor® 405

sc-7386 AF405
200 µg/ml
RMB2685.00

Are you produce from the product with cat no. SC-7386 Which is NFkb a vial 10micro gram / 50 micro liter ?

Asked by: Ahmed 1412
Thank you for your question. To request a sample size please call or email technical service . (800)-457-3801 or [email protected]
Answered by: BlakeJ
Date published: 2023-06-21

I just ordered the NFkB2 antibody (C-5) sc-7386-AF647. Can you send a FCM intracellular staining protocol? Does it work with BD cyto/fix/perm and cyto/wash buffer?

Asked by: Anonymous
Thank you for your question. You can find our flow cytometry protocol under this link: https://www.scbt.com/resources/protocols/flow-cytometry We recommend using FCM Fixation Buffer (sc-3622) and FCM Wash buffer (sc-3624) for flow cytometry.
Answered by: Tech Support Europe
Date published: 2021-08-16

Are there citations of this antibody being used in flow cytometry?

Asked by: Vidhi
Thank you for your question. We are aware of one product citation using the PE conjugated version of this antibody (sc-7386 PE) for flow cytometry. Please refer to the following product citation on PubMed for more details, PMID: 19388934.
Answered by: Technical Support
Date published: 2021-01-27

Hi, I am just wondering the data sheet for NFκB p52 (C-5): sc-7386 has only a single band which is not p52 but looks like p100. why this western results is different from the one on its website? thanks

Asked by: NF worker
Thank you for your question. The Western blot data that is currently on the data sheet is not representative of the endogenous NFKB2 protein. This is Western blot data in mouse Nfkb2 transfected 293T whole cell lysate. This transfected lysate is overepressing the full length p100 protein.
Answered by: Technical Support
Date published: 2019-07-10

Is this antibody working with the secondary m-IgGκ BP-HRP (sc-516102)?

Asked by: Pipaón
Thank you for your question. This antibody should be OK to work with the mouse-IgGk BP-HRP. If you would like to try a sample of the monoclonal antibody or the mouse IgG binding protein, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-04-24

May I know what is the packaging volume does NFκB p52 Antibody (C-5) FITC (sc-7386 FITC; 200 µg/ml) come as? How many tests can be performed using flow cytometer? Thank you

Asked by: Jackie2017
Thank you for your question. We provide the FITC-conjugated primary antibody as 200 µg in 1 mL total volume. We recommend using approximately 1 µg of FITC conjugated primary antibody for each flow cytometry experiment (based on the parameters in our protocol which is provided below). We estimate that you can use the antibody for approximately 100-200 tests. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-01-23

The WB of the C-5 clone showed a strong band in P100, how can you use this clone for IF or IHC for NFkB2 activation, normally if P52 goes up , then P100 goes down, vice versa.

Asked by: mocky
Thank you for your question. The antibody will detect p100 and/or p52, depending on what is present in the sample at the time of antibody addition. If both are present, both will be detected and you would not be able to tell them apart. I would suggest also checking your sample for the presence of one or both forms before doing your IHC or IF experiment. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-01-10

How can this mouse monoclonal antibody be used for double staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. This product, sc-7386, is available conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 9
Date published: 2017-01-09
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_9
  • loc_zh_CN, sid_7386, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent NFκB p52/p100/NFKB2 antibodyIt works really well for WB, no background. Samples are sheep thymuses.
Date published: 2023-03-17
Rated 1 out of 5 by from Not detecting right sizeWe tested human cells with both monoclonal anti-p52 and polyclonal one, which discontinued. Monoclonal antibody detected a band at ~37kD, the polyclonal one detected two bands one is p100 and the small one is about 10kD.
Date published: 2019-07-10
Rated 5 out of 5 by from p52 works well in A375 cells.I used this antibody for IF staining in A375 cells. I works well.
Date published: 2018-03-02
Rated 5 out of 5 by from Western blotThis antibody work well in western blot (gastric cancer cell line: AGS, MKN45, dilute 1:1000)
Date published: 2017-11-14
Rated 5 out of 5 by from 非常好用这支NF-kB抗体真的非常好用，之前我购买过其他品牌的这个抗体，但是还没有申请的这个小样试用装好用，条带非常漂亮，特异性好。是一个不错的抗体选择。
Date published: 2017-10-15
Rated 5 out of 5 by from the antibody worked wellThis antibody(SC-7386) worked well on WB. Sample: rat liver Dilution ratio 1:1000
Date published: 2017-09-19
Rated 5 out of 5 by from popular usedNFκB p52 and NFκB p65 is very popular used in our research .This two antibody been used for WB detect Hela cell,always strong band by 1:1000 diluted the antibody.
Date published: 2017-06-25
Rated 5 out of 5 by from see clear bandI use Mouse brain tissue extracts , used at 1:1000,no background ,but clear band seen.
Date published: 2017-06-11
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_18
  • loc_zh_CN, sid_7386, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
NFκB p52/p100/NFKB2 抗体 (C-5) is rated 4.8 out of 5 by 18.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_18
  • loc_zh_CN, sid_7386, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 114ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。