Date published: 2025-9-6
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NFATc4 Antibody (B-2) - Immunohistochemistry - Image 160213
NFATc4 抗体 (B-2): sc-271597. 福尔马林固定, 石蜡包埋的人口腔粘膜组织的免疫过氧化物酶染色显示鳞状上皮细胞的胞浆染色.
NFATc4 抗体 (B-2): sc-271597. 福尔马林固定, 石蜡包埋的人口腔粘膜组织的免疫过氧化物酶染色显示鳞状上皮细胞的胞浆染色.

NFATc4 抗体 (B-2): sc-271597

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寫評論提問

说明书
  • NFATc4 抗体 B-2 是小鼠单克隆 IgG2a κ，NFATc4抗体, 在12篇文献中引用，规格为200 µg/ml
  • 免疫human物种的NFATc4的氨基酸125-198
  • NFATc4 抗体 (B-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 NFATc4
  • 抗NFATc4抗体（B-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-271597 X, 200 µg/0.1 ml)
  • 关于如何获取NFATc4 (B-2): sc-271597的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是NFATc4 Antibody (B-2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与NFATc4 Antibody (B-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外NFAT抗体，包括NFAT5, NFATc1, NFATc2, NFATc3和NFATc4

NFATc4抗体（B-2）是一种IgG2a κ小鼠单克隆NFATc4抗体（也称为NFAT3抗体，或活化T细胞核因子4抗体），它通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的NFATc4蛋白。NFATc4抗体（B-2）既有非偶联的抗NFATc4抗体形式，也有多种偶联形式的抗NFATc4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。NFATc4（活化T细胞核因子，细胞质，钙调神经磷酸酶依赖性4）是活化T细胞DNA结合转录复合物的核因子成员，影响细胞因子基因表达、心肌肥大和脂肪细胞分化。该复合物至少由两个组分组成：一个在T细胞受体（TCR）刺激下易位到核的细胞质组分和一个诱导性核组分。该家族的其他成员参与该复合物的形成。NFATc4在T细胞中细胞因子基因的诱导表达中发挥作用，包括诱导IL-2和IL-4。p38 MAP激酶磷酸化NFATc4的NFAT同源结构域中的多个残基。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NFATc4 抗体 (B-2) 参考文献:

  1. CBP 增效需要两个 NFATc4 反式激活结构域。  |  Yang, T., et al. 2001. J Biol Chem. 276: 39569-76. PMID: 11514544
  2. p38 丝裂原活化蛋白激酶对 NFATc4 的磷酸化作用。  |  Yang, TT., et al. 2002. Mol Cell Biol. 22: 3892-904. PMID: 11997522
  3. NFATc3（而非 NFATc4）的靶向破坏揭示了钙神经蛋白介导的心脏肥大生长的内在缺陷。  |  Wilkins, BJ., et al. 2002. Mol Cell Biol. 22: 7603-13. PMID: 12370307
  4. 神经营养素和净蛋白需要钙调神经素/NFAT 信号来刺激胚胎轴突的生长。  |  Graef, IA., et al. 2003. Cell. 113: 657-70. PMID: 12787506
  5. 由转录因子 Nishéd 和 NFATc4 以及与内含子序列（内含子调控元件）结合的共激活因子 p300 组成的三元复合物在心肌肥大中对肌球蛋白轻链-2v 基因的上调起着关键作用。  |  Mathew, S., et al. 2004. J Biol Chem. 279: 41018-27. PMID: 15272022
  6. 钙神经蛋白/NFAT诱导的Fas配体/Fas死亡途径上调参与了甲基苯丙胺诱导的神经细胞凋亡。  |  Jayanthi, S., et al. 2005. Proc Natl Acad Sci U S A. 102: 868-73. PMID: 15644446
  7. 细胞外信号调节激酶/核糖体 S6 激酶信号通路对 NFATc4 转录激活复合物的招募。  |  Yang, TT., et al. 2005. Mol Cell Biol. 25: 907-20. PMID: 15657420
  8. 通过体外和体内钙调素 A beta 启动子的转录诱导来调节钙调素。  |  Oka, T., et al. 2005. Mol Cell Biol. 25: 6649-59. PMID: 16024800
  9. NFATc4在成年小鼠大脑中的免疫定位。  |  Bradley, KC., et al. 2005. J Neurosci Res. 82: 762-70. PMID: 16273547
  10. 核因子活化T细胞同种型c4（NFATc4/NFAT3）作为NMDA受体刺激皮质神经元抗凋亡转录的介质。  |  Vashishta, A., et al. 2009. J Neurosci. 29: 15331-40. PMID: 19955386
  11. 核因子活化的T细胞（NFATc4）是BDNF依赖性成年神经元存活和海马空间记忆形成所必需的。  |  Quadrato, G., et al. 2012. Proc Natl Acad Sci U S A. 109: E1499-508. PMID: 22586092
  12. 乙醇对未分化的大鼠神经祖细胞复制的抑制作用可通过绿原酸通过NFATc4/CSE信号通路来预防。  |  Shanmugam, S., et al. 2023. Alcohol Clin Exp Res (Hoboken). 47: 1530-1543. PMID: 37364904

订购信息

产品名称产品编号规格价格数量收藏夹

NFATc4 抗体 (B-2)

sc-271597
200 µg/ml
RMB2377.00

NFATc4 (B-2): m-IgG2a BP-HRP 套装

sc-545931
200 µg Ab; 10 µg BP
RMB2662.00

NFATc4 抗体 (B-2) X

sc-271597 X
200 µg/0.1 ml
RMB2377.00

NFATc4 抗体 (B-2) AC

sc-271597 AC
500 µg/ml, 25% agarose
RMB3129.00

NFATc4 抗体 (B-2) HRP

sc-271597 HRP
200 µg/ml
RMB2377.00

NFATc4 抗体 (B-2) FITC

sc-271597 FITC
200 µg/ml
RMB2482.00

NFATc4 抗体 (B-2) PE

sc-271597 PE
200 µg/ml
RMB2580.00

NFATc4 抗体 (B-2) Alexa Fluor® 488

sc-271597 AF488
200 µg/ml
RMB2685.00

NFATc4 抗体 (B-2) Alexa Fluor® 546

sc-271597 AF546
200 µg/ml
RMB2685.00

NFATc4 抗体 (B-2) Alexa Fluor® 594

sc-271597 AF594
200 µg/ml
RMB2685.00

NFATc4 抗体 (B-2) Alexa Fluor® 647

sc-271597 AF647
200 µg/ml
RMB2685.00

NFATc4 抗体 (B-2) Alexa Fluor® 680

sc-271597 AF680
200 µg/ml
RMB2685.00

NFATc4 抗体 (B-2) Alexa Fluor® 790

sc-271597 AF790
200 µg/ml
RMB2685.00

Does NFATC4 (B-2): sc-271597 antibody detect phosphorylated or dephosphrylated or total NFATC4?

Asked by: MRiaz
Thank you for your question. NFATc4 Antibody (B-2): sc-271597 detects total NFATc4, regardless of post-translational modifications.
Answered by: Tech Support Europe
Date published: 2020-12-18

Can NFATc4 (B-2): sc-271597 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, NFATc4 (B-2): sc-271597 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good AntibodyI used this on H9C2 cell lysate to detect NFATc4 levels. At the concentration of 1:1000 it did not detect anything. Only saw few bands. Might need to use a higher concentration.
Date published: 2023-03-13
Rated 5 out of 5 by from 功能很出色2022.7.20订购的该产品，使用效果很不错，实验效果呈现的很好，我很满意，以后还会多多支持该品牌。
Date published: 2022-08-03
Rated 2 out of 5 by from Used in WesternsUsed with 1:500 dilution for western boots. Had difficulty obtaining clear band with low background.
Date published: 2018-03-28
Rated 2 out of 5 by from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of 5 by from Works in WBIn western blot, NFATc4 protein could be detected by this antibody.
Date published: 2017-05-31
Rated 5 out of 5 by from perfectIt provides a strong signal with minimal background，I think that it is superior alternative to other companies’ product.
Date published: 2017-03-27
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-08
Rated 5 out of 5 by from Good WB antibody in human transfected 293TGood WB antibody in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-16
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NFATc4 抗体 (B-2) is rated 4.2 out of 5 by 9.
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