Date published: 2025-9-6
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NFATc3 Antibody (F-1) - Western Blotting - Image 1021
NFATc3 抗体 (F-1): sc-8405. Jurkat (A), BYDP (B) 和 THP-1 (C) 全细胞裂解液中 NFATc3 表达的 Western 印迹分析.
NFATc3 抗体 (F-1): sc-8405. Jurkat (A), BYDP (B) 和 THP-1 (C) 全细胞裂解液中 NFATc3 表达的 Western 印迹分析.

NFATc3 抗体 (F-1): sc-8405

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说明书
  • NFATc3抗体(F-1)是小鼠单克隆IgG1 κ NFATc3抗体, 在101篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的NFATc3的氨基酸321-395
  • NFATc3 抗体 (F-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 NFATc3
  • 抗NFATc3抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8405 X, 200 µg/0.1 ml)
  • {0} PDF格式的方案
  • 关于如何获取NFATc3 (F-1): sc-8405的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 NFATc3 抗体 (F-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NFATc3 抗体 (F-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 NFAT 抗体 包括 NFAT5, NFATc1, NFATc2, NFATc3和NFATc4

NFATc3抗体（F-1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类来源的NFATc3，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）。NFATc3 单克隆抗体（F-1）有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。NFATc3，也称为活化 T 细胞核因子 3，通过调节 T 细胞中细胞因子的表达在免疫反应中发挥关键作用。NFATc3是NFAT转录因子家族的一员，可通过细胞内钙离子浓度升高而激活。这种激活会导致钙调磷酸酶钙调神经磷酸酶对NFAT蛋白进行去磷酸化，从而发生构象变化，露出核定位信号。这种变化至关重要，因为NFAT蛋白会从细胞质转移到细胞核，并在细胞核中与DNA结合并调节基因表达。NFATc3与其他蛋白（如AP-1蛋白（Fos和Jun））的相互作用进一步强调了NFATc3在形成调节免疫反应的协同复合物中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NFATc3 抗体 (F-1) 参考文献:

  1. 识别免疫细胞激活时诱导基因中的 NFATp/AP-1 复合元素。  |  Kel, A., et al. 1999. J Mol Biol. 288: 353-76. PMID: 10329147
  2. NFAT5 是一种组成型核 NFAT 蛋白，它不与 Fos 和 Jun 合作。  |  Lopez-Rodríguez, C., et al. 1999. Proc Natl Acad Sci U S A. 96: 7214-9. PMID: 10377394
  3. NFATc1 核占位增强会导致 T 细胞活化，而与 CD28 成本刺激无关。  |  Pan, M., et al. 2007. J Immunol. 178: 4315-21. PMID: 17371988
  4. 巨噬细胞 NFATc3 可预防泡沫细胞形成和动脉粥样硬化：证据与机制  |  Liu, X., et al. 2021. Eur Heart J. 42: 4847-4861. PMID: 34570211
  5. Trim39通过作为SUMO靶向E3泛素连接酶调节转录因子NFATc3的神经细胞凋亡。  |  Basu-Shrivastava, M., et al. 2022. Cell Death Differ. 29: 2107-2122. PMID: 35449213
  6. NFATc3通过调节巨噬细胞中CCL2和CXCL2的产生促进肺部炎症和纤维化。  |  Nie, Y., et al. 2023. Aging Dis. 14: 1441-1457. PMID: 37523510
  7. NF-AT 基因家族两个新成员的分离及 NF-AT 蛋白的功能特征。  |  Hoey, T., et al. 1995. Immunity. 2: 461-72. PMID: 7749981
  8. 活化 T 细胞核因子（NFAT）基因家族成员 NFATc 新异构体的特征。  |  Park, J., et al. 1996. J Biol Chem. 271: 20914-21. PMID: 8702849
  9. 正常人 T 细胞中 NFAT 家族蛋白的表达。  |  Lyakh, L., et al. 1997. Mol Cell Biol. 17: 2475-84. PMID: 9111316
  10. NFAT 家族的转录因子：调节和功能。  |  Rao, A., et al. 1997. Annu Rev Immunol. 15: 707-47. PMID: 9143705
  11. 不同的 NFAT 家族蛋白参与了人类胸腺细胞亚群的核 NFAT-DNA 结合复合物。  |  Amasaki, Y., et al. 1998. J Immunol. 160: 2324-33. PMID: 9498773
  12. 转录因子 NF-ATc 对心脏瓣膜的形成至关重要。  |  Ranger, AM., et al. 1998. Nature. 392: 186-90. PMID: 9515964

订购信息

产品名称产品编号规格价格数量收藏夹

NFATc3 抗体 (F-1)

sc-8405
200 µg/ml
RMB2377.00

NFATc3 (F-1): m-IgG Fc BP-HRP 套装

sc-528223
200 µg Ab; 10 µg BP
RMB2662.00

NFATc3 (F-1): m-IgGκ BP-HRP 套装

sc-520549
200 µg Ab, 40 µg BP
RMB2662.00

NFATc3 (F-1): m-IgG1 BP-HRP 套装

sc-542824
200 µg Ab; 20 µg BP
RMB2662.00

NFATc3 抗体 (F-1) X

sc-8405 X
200 µg/0.1 ml
RMB2377.00

NFATc3 抗体 (F-1) AC

sc-8405 AC
500 µg/ml, 25% agarose
RMB3129.00

NFATc3 抗体 (F-1) HRP

sc-8405 HRP
200 µg/ml
RMB2377.00

NFATc3 抗体 (F-1) FITC

sc-8405 FITC
200 µg/ml
RMB2482.00

NFATc3 抗体 (F-1) PE

sc-8405 PE
200 µg/ml
RMB2580.00

NFATc3 抗体 (F-1) Alexa Fluor® 488

sc-8405 AF488
200 µg/ml
RMB2685.00

NFATc3 抗体 (F-1) Alexa Fluor® 546

sc-8405 AF546
200 µg/ml
RMB2685.00

NFATc3 抗体 (F-1) Alexa Fluor® 594

sc-8405 AF594
200 µg/ml
RMB2685.00

NFATc3 抗体 (F-1) Alexa Fluor® 647

sc-8405 AF647
200 µg/ml
RMB2685.00

NFATc3 抗体 (F-1) Alexa Fluor® 680

sc-8405 AF680
200 µg/ml
RMB2685.00

NFATc3 抗体 (F-1) Alexa Fluor® 790

sc-8405 AF790
200 µg/ml
RMB2685.00

I am planning to use sc-8405 X, 200 µg/0.1 ml antibody for ChIP. I want to know which bead to use for pull-down, protein A or protein G?

Asked by: zedsa
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-03-19

我用来检测小鼠结肠组织蛋白，但是western blot没有结果

Asked by: liyaqi
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-12-04

I would like to use sc-8405 (mouse anti-NFATc3) in IHC. Which antigen retrieval buffer was used?

Asked by: CJS300
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval.
Answered by: Technical Support
Date published: 2019-06-16

If I want to use SC-8405 conjugated with FITC in mice tissue immunofluorescence staining, what buffer should I use? Do I need to add blocking buffer?

Asked by: Book
Thank you for your question. For direct immunofluorescence staining with the FITC conjugated primary antibody (sc-8405 FITC), we recommend first blocking for 30 minutes with 10% normal blocking serum in PBS or in UltraCruz® Blocking Reagent (sc-516214). If blocking serum is used, it should ideally be derived from the same species in which the primary antibody was raised. Wash with three changes of PBS for 5 minutes each. Incubate with primary antibody conjugated to a fluorophore for 90 minutes at room temperature, or overnight at 4° C. Optimal antibody concentration should be determined by titration; recommended range is 4–10 µg/ml in either PBS with 1.5% normal blocking serum or in UltraCruz® Blocking Reagent (sc-516214). https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2023-12-08

I need to detect NFATc3 in canine samples. Can I use this antibody to do it?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2021-06-20

I am performing a double stain on my tissues. How can I do that with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using a directly conjugated mouse monoclonal primary antibodies. This product, sc-8405, is available directly conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2016-12-22
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Rated 4 out of 5 by from Used in WesternsUsed with 1:500 dilution for western boots. Mostly gives clear, specific band and low background.
Date published: 2018-03-28
Rated 1 out of 5 by from nessuna bandaHo provato il free sample ma non sono riuscita a rivelare nessuna banda su lisati di cuore totale di topo
Date published: 2017-11-13
Rated 5 out of 5 by from clear and specific bandThis antibody always give a clear and specific band at expected 130kda by Western blot.The antibody is diluted 1:1000 in 5% non-fat milk in TBST + 1:1000 NaN3. This dilution gave us satisfied signal.
Date published: 2017-07-29
Rated 5 out of 5 by from qualified productThis antibody was used dilution 1:600 and incubated at RT for 1hour , the secondary antibody was from Beyond time , goat anti mouse -HRP, The WB result got target band at 1:1000.
Date published: 2017-06-24
Rated 5 out of 5 by from good antibodyVery good antibody for WB and excellent IF result, I suggest dilution this antibody at 1:400.
Date published: 2017-05-24
Rated 5 out of 5 by from Great monoclonal antibodyNFATc3 Antibody (F-1) produced positive and specific results by WB and IHC (P)
Date published: 2017-01-11
Rated 5 out of 5 by from Strong wb and ihcOutstanding antibody for both ihc and western blot applications
Date published: 2016-12-31
Rated 4 out of 5 by from Works well in WB of J774Works well in WB of J774, L929, HEK293T and primary murine lymphoid cells - diluted 1:500
Date published: 2016-02-02
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NFATc3 抗体 (F-1) is rated 4.5 out of 5 by 13.
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