Date published: 2025-9-8

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NF90 抗体 (21): sc-136197

5.0(4)
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说明书
  • NF90 抗体 21 是小鼠单克隆 IgG1, 在4篇文献中引用,提供 50 µg/0.5 ml
  • 免疫human物种的NF90的氨基酸592-695
  • 推荐用于 human 来源的NF90 WB, IP 和 IF检测
  • 目前,我们还没有完成NF90 抗体 (21)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

NF90(活化T细胞核因子90 kDa),也被称为NFAR、DRBF、DRBP76(双链RNA结合蛋白76)、MPP4、MPHOSPH4(M期磷蛋白4)、ILF3(白细胞介素增强子结合因子3)或TCP80(翻译控制蛋白80),是一种普遍表达的核蛋白,与NF45以异二聚体的形式存在。NF90包含两个DRBM(双链RNA(dsRNA-)结合)结构域和一个DZF结构域,并且主要与NF45结合,通过结合到IL-2增强子的抗原受体反应元件(ARRE)靶序列来调节IL-2的表达。在神经细胞中,NF45/NF90异二聚体可以通过结合到编码内部核糖体进入位点(IRES)的病毒RNA的5'非翻译区来抑制人类鼻病毒2型的复制。NF45和NF90都属于双链RNA结合蛋白家族,并且都是dsRNA激活的蛋白激酶PKR的底物。由于可替代的剪接事件,NF90存在六种异构体,即NFAR-2(或ILF3-E)、NFAR-1(或DRBP76)、异构体3、DRBP76α(或ILF3-A)、DRBP76δ(也被称为DRBP76γ或ILF3-C)和异构体6。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NF90 抗体 (21) 参考文献:

  1. NF90在转录后基因调控和microRNA生物发生中的作用。  |  Masuda, K., et al. 2013. Int J Mol Sci. 14: 17111-21. PMID: 23965975
  2. NF90通过调节受感染细胞中PKR的磷酸化反应和应激颗粒发挥抗病毒活性。  |  Wen, X., et al. 2014. J Immunol. 192: 3753-64. PMID: 24623135
  3. NF45/NF90 异源二聚体有助于 60S 核糖体亚基的生物生成并影响核极形态。  |  Wandrey, F., et al. 2015. Mol Cell Biol. 35: 3491-503. PMID: 26240280
  4. NF90调节肝癌中PARP1 mRNA的稳定性。  |  Song, D., et al. 2017. Biochem Biophys Res Commun. 488: 211-217. PMID: 28487110
  5. NF90调节免疫因子表达以应对疟疾抗原。  |  Idda, ML., et al. 2019. Cell Cycle. 18: 708-722. PMID: 30784348
  6. NF90调节人类前体miRNA的加工。  |  Grasso, G., et al. 2020. Nucleic Acids Res. 48: 6874-6888. PMID: 32427329
  7. NF45/NF90 介导的 rDNA 转录为免疫抑制剂的开发提供了一个新靶点。  |  Tsai, HI., et al. 2021. EMBO Mol Med. 13: e12834. PMID: 33555115
  8. NF90 与 RISC 成分相互作用,并调节 Ago2 与 mRNA 的关联。  |  Grasso, G., et al. 2022. BMC Biol. 20: 194. PMID: 36050755
  9. NF90在RNA生物学中的多价作用。  |  Grasso, G. and Kiernan, R. 2022. Int J Mol Sci. 23: PMID: 36362366
  10. 平滑肌NF90缺乏通过FBXW7-AGER1-AGEs轴改善雄性小鼠的糖尿病动脉粥样硬化钙化。  |  Xie, F., et al. 2024. Nat Commun. 15: 4985. PMID: 38862515

订购信息

产品名称产品编号规格价格数量收藏夹

NF90 抗体 (21)

sc-136197
50 µg/0.5 ml
RMB2377.00

I am using NF90 (21): sc-136197 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from NF90 (C21) v NF90 (A3)Both antibodies worked great for immunoblot (10ug whole cell lysate), 1:1000 o/n at 4C. C21 clone superior for immunoprecipitation in CLIP lysis buffer [50mM Tris, pH 7.5, 200mM NaCl, 0.5% Sarkosyl, 0.2% Triton-X 100, 0.1% Igepal, 10% glycerol, 1mM EDTA].
Date published: 2017-11-07
Rated 5 out of 5 by from Good choice!This monoclonal NF90 Antibody (21) is a good choice for Western blot and immunofluorescence on human samples. However, we also offer an alternative clone, NF90 Antibody (A-3): sc-377406, that has stronger results in our in-house testing by Western blot, and is validated to work for mouse, rat and human samples.
Date published: 2017-01-31
Rated 5 out of 5 by from Produced exceptional immunofluorescence nuclearProduced exceptional immunofluorescence nuclear staining in WI-38 cells. -SCBT QC
Date published: 2015-02-11
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NF90抗体 (21) is rated 5.0 out of 5 by 4.
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