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NF-YA Antibody (G-2) - Western Blotting - Image 354824 — NF-YA 抗体 (G-2): sc-17753. WEHI-3 (A) 和 RAW 264. 7 (B) 全细胞裂解液中 NF-YA 表达的 Western 印迹分析.

NF-YA 抗体 (G-2): sc-17753. WEHI-3 (A) 和 RAW 264. 7 (B) 全细胞裂解液中 NF-YA 表达的 Western 印迹分析.

NF-YA 抗体 (G-2): sc-17753

Name: NF-YA抗体 (G-2)
Brand: SCBIO
SKU: sc-17753
Price: 2377.0 CNY
Availability: InStock

4.9(11)

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说明书

NF-YA 抗体 G-2 是小鼠单克隆 IgG_2a κ，NF-YA抗体, 在68篇文献中引用，规格为200 µg/ml
免疫human物种的CCAAT-binding transcription factor subunit B (NF-YA)的氨基酸139-347
NF-YA 抗体 (G-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 NF-YA; 也和以下物种反应，包括：和 equine and porcine
抗NF-YA抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
可用于凝胶迁移和ChIP的TransCruz试剂(sc-17753 X, 200 µg/0.1 ml)
关于如何获取NF-YA (G-2): sc-17753的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP, m-IgG_2a BP-HRP 和 m-IgGκ BP-HRP 是 NF-YA 抗体 (G-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NF-YA 抗体 (G-2) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(68)

快捷链接

NF-YA 抗体 (G-2) 参考文献:

NF-YA的过表达可防止谷氨酰胺缺乏。 | Dolfini, D., et al. 2020. Biochim Biophys Acta Mol Cell Res. 1867: 118571. PMID: 31706909
肺癌中的 NF-YA 过度表达：LUAD. | Bezzecchi, E., et al. 2020. Genes (Basel). 11: PMID: 32075093
代谢综合征介导ROS-miR-193b-NFYA依赖性可溶性鸟苷酸环化酶下调，并导致射血分数保持正常的心力衰竭患者出现运动诱发的肺动脉高压。 | Satoh, T., et al. 2021. Circulation. 144: 615-637. PMID: 34157861
NF-YA 诱导的 CDCA8 通过调节 MEK/ERK 通路促进肝细胞癌的进展。 | Chen, E., et al. 2023. Exp Hematol Oncol. 12: 9. PMID: 36639822
在鸟类中，NF-YA 的第 5 号外显子有不同的剪接形式。 | Gallo, A., et al. 2023. Genomics. 115: 110694. PMID: 37536396
转录因子 NFYa 在小鼠胎儿心脏发育过程中控制心肌细胞的代谢和增殖。 | Cui, M., et al. 2023. Dev Cell. 58: 2867-2880.e7. PMID: 37972593
多种 DNA 结合蛋白和多聚蛋白都含有组蛋白折叠图案。 | Baxevanis, AD., et al. 1995. Nucleic Acids Res. 23: 2685-91. PMID: 7651829
CCAAT结合因子CBF的A亚基中存在三类突变，它们划定了参与CBF-DNA复合物三步组装的功能域。 | Sinha, S., et al. 1996. Mol Cell Biol. 16: 328-37. PMID: 8524312
NF-Y 的 DNA 结合亚基三聚化结构域与高迁移率官能团蛋白 HMG-I(Y) 之间的功能相互作用。 | Currie, RA. 1997. J Biol Chem. 272: 30880-8. PMID: 9388234
对 178 个 NF-Y 结合 CCAAT 盒的调查。 | Mantovani, R. 1998. Nucleic Acids Res. 26: 1135-43. PMID: 9469818
CCAAT 结合蛋白 CBF/NF-Y 在转录中的作用 | Maity, SN. and de Crombrugghe, B. 1998. Trends Biochem Sci. 23: 174-8. PMID: 9612081
DNA 与 CBF/NF-Y 三个亚基形成复合物的途径。DNA 蛋白相互作用的光交联分析以及亚基相互作用和 DNA 结合平衡步骤的特征。 | Liang, SG. and Maity, SN. 1998. J Biol Chem. 273: 31590-8. PMID: 9813075

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
NF-YA 抗体 (G-2)	sc-17753	200 µg/ml	RMB2377.00
NF-YA (G-2): m-IgG Fc BP-HRP 套装	sc-528296	200 µg Ab; 10 µg BP	RMB2662.00
NF-YA (G-2): m-IgGκ BP-HRP 套装	sc-520637	200 µg Ab, 40 µg BP	RMB2662.00
NF-YA (G-2): m-IgG_2a BP-HRP 套装	sc-547061	200 µg Ab; 10 µg BP	RMB2662.00
NF-YA 抗体 (G-2) X	sc-17753 X	200 µg/0.1 ml	RMB2377.00
NF-YA 抗体 (G-2) AC	sc-17753 AC	500 µg/ml, 25% agarose	RMB3129.00
NF-YA 抗体 (G-2) HRP	sc-17753 HRP	200 µg/ml	RMB2377.00
NF-YA 抗体 (G-2) FITC	sc-17753 FITC	200 µg/ml	RMB2482.00
NF-YA 抗体 (G-2) PE	sc-17753 PE	200 µg/ml	RMB2580.00
NF-YA 抗体 (G-2) Alexa Fluor^® 488	sc-17753 AF488	200 µg/ml	RMB2685.00
NF-YA 抗体 (G-2) Alexa Fluor^® 546	sc-17753 AF546	200 µg/ml	RMB2685.00
NF-YA 抗体 (G-2) Alexa Fluor^® 594	sc-17753 AF594	200 µg/ml	RMB2685.00
NF-YA 抗体 (G-2) Alexa Fluor^® 647	sc-17753 AF647	200 µg/ml	RMB2685.00
NF-YA 抗体 (G-2) Alexa Fluor^® 680	sc-17753 AF680	200 µg/ml	RMB2685.00
NF-YA 抗体 (G-2) Alexa Fluor^® 790	sc-17753 AF790	200 µg/ml	RMB2685.00

I need to test the Antibody on western and immunofluorescence. Can I get the 10ug sample to test and then probably I will order the Antobody.

Asked by: Deepankar

Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.

Answered by: Tech Service

Date published: 2019-05-07

When should the TransCruz reagent sc-17753 X be used instead of the standard form sc-17753?

Asked by: Trav11

Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by jerojero from Excellent!This antibody works great in western blot analysis of NF-YA expression in WEHI-3 and RAW 264.7 whole cell lysates.

Date published: 2017-01-24

Rated 5 out of 5 by Zhongqin Liang from Strong band in WB in U251Strong band in WB in U251 and MDA-MB468 whole cell lysate

Date published: 2017-01-06

Rated 5 out of 5 by wizard1310 from Very good antibody for many applicationsWe had very good results with this antibody in Western blotting, ChIP; and IP in mouse leukemic B cell lines stably expressing the BCR-ABL oncogene. We attach a Western blot and an IP. For Western blotting and IP controls we loaded 50 ug protein and used 1:2000 dilution of the antibody for detection. For IP we started with 1ug protein and corresponding amount of antibody according to the data sheet.

Date published: 2016-12-16

Rated 4 out of 5 by MQ from Sehr guter Antik rper im WesternSehr guter Antik rper im Western. -SCBT Publication Review

Date published: 2016-02-16

Rated 5 out of 5 by ET from Good publishable data in ChIP in mouse andGood publishable data in ChIP in mouse and human 3T3-L1, HEK293 cells. -SCBT Publication Review

Date published: 2015-07-12

Rated 5 out of 5 by KG from Good specificity in WB in mouse transfectedGood specificity in WB in mouse transfected 293T whole cell lysates. -SCBT QC

Date published: 2015-06-19

Rated 5 out of 5 by JM from Strong band in WB in NIH/3T3 whole cell lysateStrong band in WB in NIH/3T3 whole cell lysate. -SCBT QC

Date published: 2015-06-16

Rated 5 out of 5 by ET from Robust signal in WB in human recombinant NFRobust signal in WB in human recombinant NF-YA fusion protein. -SCBT QC

Date published: 2015-03-17

NF-YA 抗体 (G-2) Reviews - page 2

NF-YA 抗体 (G-2) is rated 4.9 out of 5 by 11.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	NFYA	Entrez Gene ID: 4800	6p21.1	NM_002505, NM_021705	UniProt ID: P23511	189903
Mouse	Nfya	Entrez Gene ID: 18044	17 C	XM_006523791, XM_006523793, XM_006523794, XM_006523795, XM_006523796, XM_017317292, NM_001110832, NM_001347401, NM_001347402, NM_010913	UniProt ID: P23708	N/A

NF-YA 抗体 (G-2): sc-17753

NF-YA 抗体 (G-2) 参考文献:

NF-YA 抗体 (G-2) 规格

替代产品名称: Nuclear transcription factor Y subunit alpha

产品克隆名称: G-2

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG_2a kappa light chain

氨基酸: 139-347

抗原表位靶标: NF-YA

抗原表位种属: human

产品反应性/检测: NF-YA

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: equine and porcine

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 68篇各种科学出版物的引文

NF-YA 抗体 (G-2)

NF-YA (G-2): m-IgG Fc BP-HRP 套装

NF-YA (G-2): m-IgGκ BP-HRP 套装

NF-YA (G-2): m-IgG_2a BP-HRP 套装

NF-YA 抗体 (G-2) X

NF-YA 抗体 (G-2) AC

NF-YA 抗体 (G-2) HRP

NF-YA 抗体 (G-2) FITC

NF-YA 抗体 (G-2) PE

NF-YA 抗体 (G-2) Alexa Fluor^® 488

NF-YA 抗体 (G-2) Alexa Fluor^® 546

NF-YA 抗体 (G-2) Alexa Fluor^® 594

NF-YA 抗体 (G-2) Alexa Fluor^® 647

NF-YA 抗体 (G-2) Alexa Fluor^® 680

NF-YA 抗体 (G-2) Alexa Fluor^® 790

I need to test the Antibody on western and immunofluorescence. Can I get the 10ug sample to test and then probably I will order the Antobody.

When should the TransCruz reagent sc-17753 X be used instead of the standard form sc-17753?

NF-YA 抗体 (G-2): sc-17753

NF-YA 抗体 (G-2) 参考文献:

NF-YA 抗体 (G-2) 规格

替代产品名称: Nuclear transcription factor Y subunit alpha

产品克隆名称: G-2

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG2a kappa light chain

氨基酸: 139-347

抗原表位靶标: NF-YA

抗原表位种属: human

产品反应性/检测: NF-YA

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: equine and porcine

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 68篇各种科学出版物的引文

NF-YA 抗体 (G-2)

NF-YA (G-2): m-IgG Fc BP-HRP 套装

NF-YA (G-2): m-IgGκ BP-HRP 套装

NF-YA (G-2): m-IgG2a BP-HRP 套装

NF-YA 抗体 (G-2) X

NF-YA 抗体 (G-2) AC

NF-YA 抗体 (G-2) HRP

NF-YA 抗体 (G-2) FITC

NF-YA 抗体 (G-2) PE

NF-YA 抗体 (G-2) Alexa Fluor® 488

NF-YA 抗体 (G-2) Alexa Fluor® 546

NF-YA 抗体 (G-2) Alexa Fluor® 594

NF-YA 抗体 (G-2) Alexa Fluor® 647

NF-YA 抗体 (G-2) Alexa Fluor® 680

NF-YA 抗体 (G-2) Alexa Fluor® 790

I need to test the Antibody on western and immunofluorescence. Can I get the 10ug sample to test and then probably I will order the Antobody.

When should the TransCruz reagent sc-17753 X be used instead of the standard form sc-17753?

抗体同型: IgG_2a kappa light chain

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

NF-YA (G-2): m-IgG_2a BP-HRP 套装

NF-YA 抗体 (G-2) Alexa Fluor^® 488

NF-YA 抗体 (G-2) Alexa Fluor^® 546

NF-YA 抗体 (G-2) Alexa Fluor^® 594

NF-YA 抗体 (G-2) Alexa Fluor^® 647

NF-YA 抗体 (G-2) Alexa Fluor^® 680

NF-YA 抗体 (G-2) Alexa Fluor^® 790