Date published: 2025-9-8
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NF-H Antibody (D-9) - Western Blotting - Image 355611
NF-H 抗体 (D-9): sc-137009. EOC 20 全细胞裂解液 (A) 和大鼠脊髓组织提取物 (B) 中 NF-H 表达的 Western 印迹分析.
NF-H 抗体 (D-9): sc-137009. EOC 20 全细胞裂解液 (A) 和大鼠脊髓组织提取物 (B) 中 NF-H 表达的 Western 印迹分析.

NF-H 抗体 (D-9): sc-137009

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说明书
  • NF-H抗体(D-9)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-100，在NF-H的human起源的N-terminus处进行映射
  • 推荐用于 mouse, rat 和 human 来源的NF-H WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 NF-H 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NF-H 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 NF 抗体包括 NF-H, NF-L 和 NF-M

神经丝蛋白H（NF-H）是神经丝重多肽的简称，属于中间丝蛋白家族的一员，是神经元细胞骨架的主要组成部分。神经丝是动态的结构；它们包含大量蛋白激酶的磷酸化位点，包括蛋白激酶A、蛋白激酶C、细胞周期蛋白依赖性激酶5、细胞外信号调节激酶、糖原合成酶激酶-3以及应激激活的蛋白激酶γ。除了控制轴突口径的作用外，神经丝还可能影响其他细胞骨架元素，如微管和肌动蛋白丝。在神经退行性疾病中，经常可以观察到神经丝磷酸化或代谢的变化，这些疾病包括肌萎缩侧索硬化症（ALS）、帕金森病和阿尔茨海默病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NF-H 抗体 (D-9) 参考文献:

  1. 细胞周期蛋白依赖性激酶-5（CDK-5）使神经丝重链（NF-H）磷酸化，从而产生抗体的表位，标记肌萎缩侧索硬化症（ALS）中的神经丝积聚，并存在于ALS中受影响的运动神经元中。  |  Bajaj, NP., et al. 1999. Prog Neuropsychopharmacol Biol Psychiatry. 23: 833-50. PMID: 10509378
  2. 小鼠神经丝亚基基因缺失的轴突的电生理特性：NF-H缺失时传导速度和轴突直径之间的差异。  |  Kriz, J., et al. 2000. Brain Res. 885: 32-44. PMID: 11121527
  3. 检测血清中的神经丝-H，作为预测轻度脑外伤患者损伤严重程度的诊断工具。  |  Gatson, JW., et al. 2014. J Neurosurg. 121: 1232-8. PMID: 25192482
  4. 阿米替林治疗对抑郁症实验模型中神经丝-H 蛋白的影响  |  Sanna, MD., et al. 2017. Brain Res Bull. 128: 1-6. PMID: 27818278
  5. 编码大鼠高分子量神经丝肽（NF-H）的cDNA克隆：大鼠发育和组织表达，以及其人类同源物在染色体1和22上的定位。  |  Lieberburg, I., et al. 1989. Proc Natl Acad Sci U S A. 86: 2463-7. PMID: 2928342
  6. 人类重神经丝亚基（NF-H）的结构和组织以及编码它的基因。  |  Lees, JF., et al. 1988. EMBO J. 7: 1947-55. PMID: 3138108
  7. 作为致命性创伤性脑损伤的替代标记，死后脑脊液中的髓磷脂碱性蛋白和神经丝H。  |  Bohnert, S., et al. 2021. Int J Legal Med. 135: 1525-1535. PMID: 33895854
  8. 由cAMP依赖性蛋白激酶催化亚基对天然和重组的NF-L、NF-M和NF-H组成的神经丝进行磷酸化。  |  Hisanaga, S., et al. 1994. Mol Biol Cell. 5: 161-72. PMID: 8019002
  9. 对神经丝重链蛋白NF-H的不同亚型进行磷酸化和解磷酸化。  |  Chertoff, R., et al. 1995. Cell Mol Neurobiol. 15: 269-81. PMID: 8590456
  10. 不同重神经丝亚基（NF-H）高度磷酸化重复域的分离和表征。  |  Soussan, L., et al. 1996. Cell Mol Neurobiol. 16: 463-77. PMID: 8879749

订购信息

产品名称产品编号规格价格数量收藏夹

NF-H 抗体 (D-9)

sc-137009
200 µg/ml
RMB2377.00

NF-H (D-9): m-IgG Fc BP-HRP 套装

sc-539916
200 µg Ab; 10 µg BP
RMB2662.00

NF-H (D-9): m-IgG1 BP-HRP 套装

sc-541784
200 µg Ab; 20 µg BP
RMB2662.00

Can NF-H (D-9): sc-137009 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, NF-H (D-9): sc-137009 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from strong signalsNF-H antibody has a good effect in immunoblotting and 1:1000 diluted bands are strong and clear.
Date published: 2017-10-25
Rated 5 out of 5 by from good workingThe antibody detect NF-H in mosue brain , I suggest transferred the protein onto PVDF membranes with a semi-dry transfer method, incubate the antibody with the membrance for 1hour at RT ,the expose time canbe controled at 1-5min.
Date published: 2017-09-03
Rated 5 out of 5 by from nice stainingVery pleased with IHC data; clear, specific, very nice staining.
Date published: 2017-06-03
Rated 5 out of 5 by from specific signalStrong and specific signal detected with NF-H by WB in human brain samples.
Date published: 2017-04-30
Rated 5 out of 5 by from Excellentband in Western Blot in human cathepsin K transfected EOC 20 whole cell lysate,good antibody and successful experiment.
Date published: 2016-12-17
Rated 5 out of 5 by from WB and IF publishable data with mouse lumbarWB and IF publishable data with mouse lumbar spinal cord - SCBT Publication Review
Date published: 2015-05-20
Rated 5 out of 5 by from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-11
Rated 5 out of 5 by from Produced beautiful immunoperoxidase neurofilamentProduced beautiful immunoperoxidase neurofilament staining in formalin fixed, paraffin-embedded human brain tissue. -SCBT QC
Date published: 2013-06-21
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NF-H 抗体 (D-9) is rated 5.0 out of 5 by 8.
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