Date published: 2025-9-7
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Neutrophil Elastase Antibody (NP57) - Western Blotting - Image 361490
中性粒细胞弹性蛋白酶抗体 (NP57): sc-53388. U-937 全细胞裂解液中中性粒细胞弹性蛋白酶表达的 Western 印迹分析.
Neutrophil Elastase Antibody (NP57) - Western Blotting - Image 361490
中性粒细胞弹性蛋白酶抗体 (NP57): sc-53388. U-937 全细胞裂解液中中性粒细胞弹性蛋白酶表达的 Western 印迹分析.
中性粒细胞弹性蛋白酶抗体 (NP57): sc-53388. U-937 全细胞裂解液中中性粒细胞弹性蛋白酶表达的 Western 印迹分析.

Neutrophil Elastase 抗体 (NP57): sc-53388

4.7(3)
寫評論提問

说明书
  • Neutrophil Elastase抗体(NP57)是小鼠单克隆IgG1 κ, 在17篇文献中引用，规格为200 µg/ml
  • 针对human来源的中性粒细胞颗粒蛋白
  • Neutrophil Elastase 抗体 (NP57) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 Neutrophil Elastase
  • 抗Neutrophil Elastase抗体（NP57）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Neutrophil Elastase (NP57): sc-53388的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Neutrophil Elastase 抗体 (NP57) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Neutrophil Elastase 抗体 (NP57) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Neutrophil Elastase 抗体包括 Neutrophil Marker

中性粒细胞弹性蛋白酶抗体（NP57）是一种小鼠单克隆IgG1 kappa轻链抗体，专门针对人源中性粒细胞颗粒蛋白。中性粒细胞弹性蛋白酶单克隆抗体（NP57）可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）等方法有效检测人类中性粒细胞弹性蛋白酶，该酶也称为ELANE或髓蛋白酶。中性粒细胞弹性蛋白酶（NP57）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®染料，可满足各种实验需求。中性粒细胞弹性蛋白酶是一种重要的丝氨酸蛋白酶，参与吞噬蛋白的蛋白水解，促进中性粒细胞迁移和损伤后的组织重塑。中性粒细胞弹性蛋白酶在多种炎症性疾病（如特发性肺纤维化、类风湿性关节炎、成人呼吸窘迫综合征和囊性纤维化）的进展中起着重要作用。中性粒细胞弹性蛋白酶通过降解弹性蛋白、蛋白多糖和IV型胶原蛋白等细胞外基质成分，对正常的生理过程和病理组织破坏都有影响。中性粒细胞弹性蛋白酶与补体系统相互作用，可裂解抗凝血酶和纤维蛋白原等蛋白质，并作为有效的血小板激动剂，增强血小板聚集、分泌和钙动员。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Neutrophil Elastase 抗体 (NP57)

sc-53388
200 µg/ml
RMB2377.00

Neutrophil Elastase (NP57): m-IgG Fc BP-HRP 套装

sc-526804
200 µg Ab; 10 µg BP
RMB2662.00

Neutrophil Elastase (NP57): m-IgG1 BP-HRP 套装

sc-532177
200 µg Ab; 20 µg BP
RMB2662.00

Neutrophil Elastase 抗体 (NP57) AC

sc-53388 AC
500 µg/ml, 25% agarose
RMB3129.00

Neutrophil Elastase 抗体 (NP57) HRP

sc-53388 HRP
200 µg/ml
RMB2377.00

Neutrophil Elastase 抗体 (NP57) FITC

sc-53388 FITC
200 µg/ml
RMB2482.00

Neutrophil Elastase 抗体 (NP57) PE

sc-53388 PE
200 µg/ml
RMB2580.00

Neutrophil Elastase 抗体 (NP57) Alexa Fluor® 488

sc-53388 AF488
200 µg/ml
RMB2685.00

Neutrophil Elastase 抗体 (NP57) Alexa Fluor® 546

sc-53388 AF546
200 µg/ml
RMB2685.00

Neutrophil Elastase 抗体 (NP57) Alexa Fluor® 594

sc-53388 AF594
200 µg/ml
RMB2685.00

Neutrophil Elastase 抗体 (NP57) Alexa Fluor® 647

sc-53388 AF647
200 µg/ml
RMB2685.00

Neutrophil Elastase 抗体 (NP57) Alexa Fluor® 680

sc-53388 AF680
200 µg/ml
RMB2685.00

Neutrophil Elastase 抗体 (NP57) Alexa Fluor® 790

sc-53388 AF790
200 µg/ml
RMB2685.00

Do you have optimal concentration for flow testing for this product? (Cat #: sc-53388; Lot #: A1521)

Asked by: Mary C
Thank you for your question. We recommend using 1 µg of antibody in 100 µl of cell suspension. You can find a detailed protocol for FCM by clicking the following link. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: Tech Support Europe
Date published: 2022-03-17

Has a conjugated anti-NE antibody been used in Flow, and in which case do you have the isotype control available? Thanks

Asked by: RobF
Thank you for your question. This antibody is a good choice for Flow cytometry, it's warrantied and recommended for this use. You can find our isotype control IgGs here: https://www.scbt.com/browse/Control-IgGs-and-IgG-Conjugates/_/N-1j3ajah
Answered by: Tech Service
Date published: 2020-01-14

What is the recommended secondary for Neutrophil Elastase (NP57): sc-53388 monoclonal antibody?

Asked by: Trav11
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Very good antibodyAntibody gives a strong and specific staining on frozen tissue sections
Date published: 2018-06-07
Rated 5 out of 5 by from Produced publishable WB, IF and IHC data on humanThis Neutrophil Elastase monoclonal produced several citations for use in WB, IF and IHC on human samples.
Date published: 2017-01-20
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Neutrophil Elastase 抗体 (NP57) is rated 4.7 out of 5 by 3.
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