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neuropilin-1 抗体 (A-12): sc-5307

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说明书
  • neuropilin-1 抗体 A-12 是小鼠单克隆 IgG1 κ,neuropilin-1抗体, 在65篇文献中引用,规格为200 µg/ml
  • 免疫human物种的neuropilin的氨基酸570-855
  • neuropilin-1 抗体 (A-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 neuropilin
  • 抗neuropilin-1抗体(A-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是neuropilin-1 Antibody (A-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与neuropilin-1 Antibody (A-12) 打包提供(请参阅下面的订购信息)。
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神经丝蛋白抗体(A-12)是一种小鼠单克隆IgG1 kappa轻链抗体,专门针对神经丝蛋白,也称为NRP1或神经丝蛋白-1,来源于人、大鼠和小鼠。抗神经丝蛋白抗体(A-12)可识别与人类神经丝蛋白的氨基酸570-855相对应的表位,建议用于蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)。神经丝蛋白-1是一种I型跨膜受体,通过引导轴突生长在神经发育中起着至关重要的作用,同时也是III类趋化因子和血管内皮生长因子(VEGF)的高亲和力受体,在轴突引导和血管生成中起着关键作用。神经丝蛋白-1主要表达于动脉内皮细胞,与结构特征相似的近亲神经丝蛋白-2不同,后者存在于静脉和淋巴内皮细胞中。这些神经丝蛋白的不同表达有助于其在血管发育和免疫细胞功能中发挥独特的作用。神经丝蛋白单克隆抗体(A-12)有未结合和结合两种形式,包括与琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®染料结合的变体,为各种实验应用提供了灵活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

neuropilin-1 抗体 (A-12) 参考文献:

  1. 神经肽在生理和病理血管生成中的作用  |  Staton, CA., et al. 2007. J Pathol. 212: 237-48. PMID: 17503412
  2. [血管内皮生长因子受体在皮肤病中的表达研究进展]。  |  Yang, XH., et al. 2007. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 29: 279-82. PMID: 17536285
  3. 聚ialylated neuropilin-2 在人类树突状细胞表面表达并调节树突状细胞与 T 淋巴细胞的相互作用。  |  Curreli, S., et al. 2007. J Biol Chem. 282: 30346-56. PMID: 17699524
  4. 神经丝蛋白-1促进SARS-CoV-2细胞进入和感染。  |  Cantuti-Castelvetri, L., et al. 2020. Science. 370: 856-860. PMID: 33082293
  5. 神经丝蛋白(NRPs)相关病理状况及其调节剂。  |  Broz, M., et al. 2022. Int J Mol Sci. 23: PMID: 35955539
  6. 小鼠半aphorin D/collapsin是一个多样化基因家族的成员,可创建抑制轴突延伸的结构域。  |  Püschel, AW., et al. 1995. Neuron. 14: 941-8. PMID: 7748561
  7. Semaphorin III 可作为一种选择性趋光剂,对脊髓中的感觉投射进行模式化。  |  Messersmith, EK., et al. 1995. Neuron. 14: 949-59. PMID: 7748562
  8. 轴突导向:排斥生长锥的有力证据  |  Dodd, J. and Schuchardt, A. 1995. Cell. 81: 471-4. PMID: 7758101
  9. semaphorin基因编码一系列跨膜和分泌型生长锥导向分子。  |  Kolodkin, AL., et al. 1993. Cell. 75: 1389-99. PMID: 8269517
  10. 引导分子 semaphorin III 表达于脊髓和外周区域,这些区域被不断生长的感觉轴突所避开。  |  Wright, DE., et al. 1995. J Comp Neurol. 361: 321-33. PMID: 8543665
  11. Neuropilin 是轴突趋化素 Semaphorin III 的受体。  |  He, Z. and Tessier-Lavigne, M. 1997. Cell. 90: 739-51. PMID: 9288753
  12. Neuropilin 是一种 semaphorin III 受体。  |  Kolodkin, AL., et al. 1997. Cell. 90: 753-62. PMID: 9288754

订购信息

产品名称产品编号规格价格数量收藏夹

neuropilin-1 抗体 (A-12)

sc-5307
200 µg/ml
CNY2422.00

neuropilin-1 (A-12): m-IgG Fc BP-HRP 套装

sc-528159
200 µg Ab; 10 µg BP
CNY2715.00

neuropilin-1 (A-12): m-IgGκ BP-HRP 套装

sc-520467
200 µg Ab, 40 µg BP
CNY2715.00

neuropilin-1 (A-12): m-IgG1 BP-HRP 套装

sc-542792
200 µg Ab; 20 µg BP
CNY2715.00

neuropilin-1 抗体 (A-12) AC

sc-5307 AC
500 µg/ml, 25% agarose
CNY3189.00

neuropilin-1 抗体 (A-12) HRP

sc-5307 HRP
200 µg/ml
CNY2422.00

neuropilin-1 抗体 (A-12) FITC

sc-5307 FITC
200 µg/ml
CNY2527.00

neuropilin-1 抗体 (A-12) PE

sc-5307 PE
200 µg/ml
CNY2625.00

neuropilin-1 抗体 (A-12) Alexa Fluor® 488

sc-5307 AF488
200 µg/ml
CNY2738.00

neuropilin-1 抗体 (A-12) Alexa Fluor® 546

sc-5307 AF546
200 µg/ml
CNY2738.00

neuropilin-1 抗体 (A-12) Alexa Fluor® 594

sc-5307 AF594
200 µg/ml
CNY2738.00

neuropilin-1 抗体 (A-12) Alexa Fluor® 647

sc-5307 AF647
200 µg/ml
CNY2738.00

neuropilin-1 抗体 (A-12) Alexa Fluor® 680

sc-5307 AF680
200 µg/ml
CNY2738.00

neuropilin-1 抗体 (A-12) Alexa Fluor® 790

sc-5307 AF790
200 µg/ml
CNY2738.00

Buenas tardes. Realizo un proyecto de investigación y me gustaría adquirir una muestra gratuita de este anticuerpo(neuropilina Anticuerpo (A-12): sc-5307). ¿Qué proceso debo realizar? Saludos.

Asked by: Margarita
Gracias por tu pregunta. Comuníquese directamente con el servicio técnico para solicitar un vial de 10 µg gratis de este anticuerpo.
Answered by: Tech Support Europe
Date published: 2021-03-23

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-5307 HRP), phycoerythrin (sc-5307 PE), fluoroscein (sc-5307 FITC), Alexa Fluor® 488 (sc-5307 AF488) and Alexa Fluor® 647 (sc-5307 AF647).
Answered by: Tech Service
Date published: 2017-01-27
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Rated 3 out of 5 by ifi15 from funziona su linee cellulari, non su tessuti umaniUna linea di controllo di ALCL umana è venuta effettivamente positiva. Ho provato altri stainings su colon umano e non ho avuto buoni risultati
Date published: 2025-06-09
Rated 1 out of 5 by DChan from This NRP1 antibody does not workThe title says it all. Same membrane blot probe sequentially: SCBT mAb first, then probed with mAb from another company. With 3 CRSPR guide RNA that target NRP1 in two cell lines, I can say unequivocally the NRP1 antibody lot I received (lot #E0523) does not work.
Date published: 2024-07-02
Rated 5 out of 5 by manash from great resultsI received free samples and tried on western blotting. Tha bands are thick with no secondary bands. Need to dilute antibody concentrations to get good data.
Date published: 2018-02-12
Rated 5 out of 5 by PL61 from Anticorpo versatile ed altamente specificoHo utilizzato questo anticorpo sia per western blot che per neutralizzazione della NRP1 in cellule di melanoma umano con ottimi risultati.
Date published: 2018-02-10
Rated 5 out of 5 by PECAM1 from PDGFC and neuropilin-1 in melanomaThe antibody has allowed to demonstrate for the first time the capability of NRP-1 expressing melanoma cells to be stimulated by PDGFC
Date published: 2018-01-16
Rated 1 out of 5 by RGCSM from Aucun signal sur des cellules exprimant NRP1Lyse Laemmli ou bien RIPA de cellules 786-O ou HUVEC exprimant NRP1 et NRP2. Les mêmes extraits ont été déposés sur gel WB 9% puis la membrane hybridée avec différents anticorps contre NRP1 (différents fournisseurs). Seul le Santa Cruz ne donne pas de signal
Date published: 2017-11-30
Rated 5 out of 5 by Germaine from Highly recommended for IHC(P) and WB.This is a well characterized monoclonal antibody with tons of citations in the literature for a wide variety of applications. Great Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue, and Immunofluorescence staining of methanol-fixed HeLa cells.
Date published: 2016-12-23
Rated 5 out of 5 by NZ from Published data for WB and IF in human kidneyPublished data for WB and IF in human kidney and PTC cells. -SCBT Publication Review
Date published: 2015-05-15
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neuropilin-1 抗体 (A-12) is rated 4.2 out of 5 by 13.
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