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Neurogenin 1 Antibody (OR-7) - Western Blotting - Image 33709
神经原蛋白 1 抗体 (OR-7): sc-100332. HeLa 核提取物中神经原蛋白 1 表达的 Western 印迹分析.
Neurogenin 1 Antibody (OR-7) - Western Blotting - Image 33709
神经原蛋白 1 抗体 (OR-7): sc-100332. HeLa 核提取物中神经原蛋白 1 表达的 Western 印迹分析.
Neurogenin 1 Antibody (OR-7) - Immunofluorescence - Image 35530
神经原蛋白 1 抗体 (OR-7): sc-100332. 免疫荧光染色多聚甲醛固定的 HeLa 细胞, 显示核定位.
神经原蛋白 1 抗体 (OR-7): sc-100332. HeLa 核提取物中神经原蛋白 1 表达的 Western 印迹分析.

Neurogenin 1 抗体 (OR-7): sc-100332

3.0(5)
寫評論提問

说明书
  • Neurogenin 1 抗体 OR-7 是小鼠单克隆 IgG2a κ，Neurogenin 1抗体, 在3篇文献中引用，规格为50 µg/0.5 ml
  • 免疫human物种的重组Neurogenin 1
  • 推荐用于 mouse, rat 和 human 来源的Neurogenin 1 WB, IP, IF 和 ELISA检测
  • 关于如何获取Neurogenin 1 (OR-7): sc-100332的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Neurogenin 1 抗体 (OR-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Neurogenin 抗体 包括 Neurogenin 1, Neurogenin 2和Neurogenin 3

Neurogenin 1抗体（OR-7）是一种IgG2a κ小鼠单克隆Neurogenin 1抗体（也称为Neurogenin 1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Neurogenin 1蛋白。Neurogenin 1抗体（OR-7）为非偶联形式的抗Neurogenin 1抗体。Neurogenin蛋白家族属于碱性螺旋-环-螺旋（bHLH）超家族，由Neurogenin 1、Neurogenin 2和Neurogenin 3（也称为ngn3）组成。bHLH成员是决定细胞命运的转录调节因子。在小鼠神经发生过程中，Neurogenin 1和Neurogenin 2在中枢和外周神经系统的不同祖细胞群中表达。靶向突变分析表明，Neurogenin 1对于近端颅感觉神经节的神经元前体的决定至关重要，而Neurogenin 2对于外胚层基板衍生的感觉神经元前体的决定至关重要。编码Neurogenin 1的基因位于人类染色体5q23-q31上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Neurogenin 1 抗体 (OR-7) 参考文献:

  1. 胰腺四个内分泌细胞系的发育都需要神经原蛋白3。  |  Gradwohl, G., et al. 2000. Proc Natl Acad Sci U S A. 97: 1607-11. PMID: 10677506
  2. 神经原蛋白 3 对胰岛特异性基因 BETA2 (neuroD) 的调控。  |  Huang, HP., et al. 2000. Mol Cell Biol. 20: 3292-307. PMID: 10757813
  3. 转录因子肝细胞核因子 6 调节胰腺内分泌细胞分化并控制前内分泌基因 ngn3 的表达。  |  Jacquemin, P., et al. 2000. Mol Cell Biol. 20: 4445-54. PMID: 10825208
  4. 胰腺α细胞和β细胞从表达神经原蛋白3的前体中独立发育：notch通路在抑制过早分化中的作用。  |  Jensen, J., et al. 2000. Diabetes. 49: 163-76. PMID: 10868931
  5. 神经原蛋白3的表达揭示了胰腺中的胰岛细胞前体群体。  |  Schwitzgebel, VM., et al. 2000. Development. 127: 3533-42. PMID: 10903178
  6. neurogenin1对于确定颅内感觉神经节近端神经元前体至关重要。  |  Ma, Q., et al. 1998. Neuron. 20: 469-82. PMID: 9539122
  7. bHLH 蛋白 NEUROGENIN 2 是支气管胎座源性感觉神经元的决定因子。  |  Fode, C., et al. 1998. Neuron. 20: 483-94. PMID: 9539123

订购信息

产品名称产品编号规格价格数量收藏夹

Neurogenin 1 抗体 (OR-7)

sc-100332
50 µg/0.5 ml
RMB2504.00

I am using Neurogenin 1 (OR-7): sc-100332 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24

Has anyone try this antibody for immunoprecipitation of endogenous protein? Thanks!

Asked by: Raquel
Thank you for your question. We are not aware of anyone publishing with Neurogenin 1 Antibody (OR-7): sc-100332 for immunoprecipitation. We do warranty the antibody to work for this research application based on the performance of similar antibodies and the reactivity of this antibody in Western blot. Please contact our Technical Service Department for further support. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Technical Support
Date published: 2017-01-19
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Rated 2 out of 5 by from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 1 out of 5 by from Did not work for immunohistochemistryI tried this antibody at a 1:50 and 1:500 dilution, with or without TSA-based signal amplification, on adult mouse brain sections (using blocking buffer with 0.3% Triton-X100, over-night incubation of primary at 4'C). It didn't work.
Date published: 2018-01-18
Rated 4 out of 5 by from This has worked well in IHCThis has worked well in IHC.
Date published: 2015-10-27
Rated 4 out of 5 by from Nice band seen in WB using HeLa nuclear extractNice band seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-06-23
Rated 4 out of 5 by from Produced good immunofluorescence nuclearProduced good immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-02
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Neurogenin 1 抗体 (OR-7) is rated 3.0 out of 5 by 5.
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