Date published: 2025-9-8
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Neuro D Antibody (A-10) - Western Blotting - Image 353343
Neuro D 抗体 (A-10): sc-46684. IMR-32 全细胞裂解液中 Neuro D 表达的 Western 印迹分析.
Neuro D 抗体 (A-10): sc-46684. IMR-32 全细胞裂解液中 Neuro D 表达的 Western 印迹分析.

Neuro D 抗体 (A-10): sc-46684

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说明书
  • Neuro D 抗体 A-10 是小鼠单克隆 IgG1 κ，Neuro D抗体, 在21篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Neuro D的氨基酸281-356
  • Neuro D 抗体 (A-10) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Neuro D
  • 抗Neuro D抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-46684 X, 200 µg/0.1 ml)
  • 关于如何获取Neuro D (A-10): sc-46684的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Neuro D Antibody (A-10) for WB applications. 的首选辅助检测试剂，这些试剂现与Neuro D Antibody (A-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Neuro D抗体，包括Neuro D2

神经D抗体（A-10）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测人类神经D蛋白，也称为NEUROD1或神经分化1。抗神经D抗体（A-10）靶向神经D蛋白281-356氨基酸的表位，确保在应用中（如：蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA））的准确识别。Neuro D是一种重要的基本螺旋-环-螺旋（bHLH）转录因子，参与神经分化，在神经元在中枢和周围神经系统中的发育和最终分化中发挥着重要作用。通过调节基因表达，Neuro D对细胞类型确定和成熟至关重要，而细胞类型确定和成熟是神经元正常功能和大脑整体发育的基本过程。Neuro D (A-10)单克隆抗体有多种结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足各种实验需求。无论是以非结合形式还是与特定标签结合使用，Neuro D (A-10)抗体都能有效促进对Neuro D在神经发生中的作用以及诱导神经前体细胞分化成成熟神经元的能力的研究，正如在非洲爪蟾胚胎等模式生物中驱动神经元分化所证明的那样。Neuro D (A-10) 单克隆抗体可靠且经过验证，是研究人员探索神经元发育机制和由转录因子（如Neuro D）控制的细胞分化复杂过程不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Neuro D 抗体 (A-10) 参考文献:

  1. ASCL1和NEUROD1揭示肺神经内分泌肿瘤的异质性并调控不同的遗传程序。  |  Borromeo, MD., et al. 2016. Cell Rep. 16: 1259-1272. PMID: 27452466
  2. 先驱因子NeuroD1重整转录物和表观遗传学图谱，执行小胶质细胞-神经元转换。  |  Matsuda, T., et al. 2019. Neuron. 101: 472-485.e7. PMID: 30638745
  3. 基于 NeuroD1 AAV 的基因疗法通过体内星形胶质细胞到神经元的转化实现缺血性损伤后的脑功能修复。  |  Chen, YC., et al. 2020. Mol Ther. 28: 217-234. PMID: 31551137
  4. 由ASCL1、NEUROD1、POU2F3和YAP1定义的SCLC亚型：免疫组化和组织病理学的综合表征。  |  Baine, MK., et al. 2020. J Thorac Oncol. 15: 1823-1835. PMID: 33011388
  5. NeuroD1 可诱导小胶质细胞凋亡，但不能诱导小胶质细胞到神经元的跨系重编程。  |  Rao, Y., et al. 2021. Neuron. 109: 4094-4108.e5. PMID: 34875233
  6. 给蛛网膜下腔出血小鼠模型注射 NeuroD1 可改善神经炎症并促进神经发生。  |  Chen, P., et al. 2023. J Neuroinflammation. 20: 261. PMID: 37953259
  7. 基本螺旋环螺旋蛋白 NeuroD 将爪蟾外胚层转化为神经元。  |  Lee, JE., et al. 1995. Science. 268: 836-44. PMID: 7754368
  8. 肌源性 bHLH 转录因子与钙结合钙调蛋白和 S100a（α alpha）蛋白的相互作用。  |  Baudier, J., et al. 1995. Biochemistry. 34: 7834-46. PMID: 7794894
  9. 肌原蛋白 bHLH 基因 MRF4 失活会导致肌原蛋白上调和肋骨异常。  |  Zhang, W., et al. 1995. Genes Dev. 9: 1388-99. PMID: 7797078
  10. 转录因子 E47 的晶体结构：基本区螺旋-环-螺旋二聚体对 E 框的识别。  |  Ellenberger, T., et al. 1994. Genes Dev. 8: 970-80. PMID: 7926781
  11. MyoD bHLH 结构域-DNA 复合物的晶体结构：DNA 识别的视角及对转录激活的影响。  |  Ma, PC., et al. 1994. Cell. 77: 451-9. PMID: 8181063
  12. Max 通过二聚体 b/HLH/Z 结构域识别其同源 DNA。  |  Ferré-D'Amaré, AR., et al. 1993. Nature. 363: 38-45. PMID: 8479534

订购信息

产品名称产品编号规格价格数量收藏夹

Neuro D 抗体 (A-10)

sc-46684
200 µg/ml
RMB2377.00

Neuro D (A-10): m-IgG Fc BP-HRP 套装

sc-526726
200 µg Ab; 10 µg BP
RMB2662.00

Neuro D (A-10): m-IgG1 BP-HRP 套装

sc-532099
200 µg Ab; 20 µg BP
RMB2662.00

Neuro D 抗体 (A-10) X

sc-46684 X
200 µg/0.1 ml
RMB2377.00

Neuro D 抗体 (A-10) AC

sc-46684 AC
500 µg/ml, 25% agarose
RMB3129.00

Neuro D 抗体 (A-10) HRP

sc-46684 HRP
200 µg/ml
RMB2377.00

Neuro D 抗体 (A-10) FITC

sc-46684 FITC
200 µg/ml
RMB2482.00

Neuro D 抗体 (A-10) PE

sc-46684 PE
200 µg/ml
RMB2580.00

Neuro D 抗体 (A-10) Alexa Fluor® 488

sc-46684 AF488
200 µg/ml
RMB2685.00

Neuro D 抗体 (A-10) Alexa Fluor® 546

sc-46684 AF546
200 µg/ml
RMB2685.00

Neuro D 抗体 (A-10) Alexa Fluor® 594

sc-46684 AF594
200 µg/ml
RMB2685.00

Neuro D 抗体 (A-10) Alexa Fluor® 647

sc-46684 AF647
200 µg/ml
RMB2685.00

Neuro D 抗体 (A-10) Alexa Fluor® 680

sc-46684 AF680
200 µg/ml
RMB2685.00

Neuro D 抗体 (A-10) Alexa Fluor® 790

sc-46684 AF790
200 µg/ml
RMB2685.00

Dear Sir, do you know if this antibody reacts with mouse NeuroD??

Asked by: Lorena Varela
Thank you for your question. We do not yet have data confirming reactivity in mouse samples, and so this is not a recommended species reactivity at this time. As an alternative, we would recommend a different antibody, Neuro D (G-12): sc-398891, which is recommended for use in mouse, rat and human samples.
Answered by: Technical Support
Date published: 2019-01-26

Has this antibody been tested with PE for FACS? Concerned about the size of PE considering NeuroD1 is a intranuclear protein.

Asked by: Mart
Thank you for your question. We recommend the AlexaFluor conjugate of this antibody for flow cytometry. You can find our recommended protocol for flow cytometry by following this link: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

When should the TransCruz reagent sc-46684 X be used instead of the standard form sc-46684?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from IHC(P) in mouseNo staining. The antibody was evaluated in immunohistochemistry of paraffin-embedded formalin-fixed mouse tissue after antigen retrieval.
Date published: 2019-02-07
Rated 1 out of 5 by from Western blotI have used this antibody to do western blot. We tried different dilutions but we couldn't see any band
Date published: 2018-03-15
Rated 5 out of 5 by from Worked really well.On western blot, it gave a clean band. Expression pattern amongst cell lines match RNA data and results with the old Santa Cruz goat polyclonal.
Date published: 2017-10-13
Rated 4 out of 5 by from Good antibody!It worked for us very well after antigen retrieval in citrate buffer.
Date published: 2017-10-05
Rated 5 out of 5 by from Expected resultI have used it for IF on brain sections of adult mice, with the dilution 1:100, it worked well, staining cytoplasm,expected staining.
Date published: 2017-08-26
Rated 5 out of 5 by from woked very wellThis antibody is very well for Neuro D analyisis, we tried twice, I suggest use 1:1000 dilution for high expression sample such as positive control.
Date published: 2017-08-12
Rated 5 out of 5 by from western blotI have used this antibody to do western blot on mouse kidney tissue lysates. The antibody(1:1000) been incubated at 4 °C over night, then with secondary antibody goat anti mouse IgG -HRP at room temperature for 1 hour and exposed 1-5min, The antibody gives the right target band ,it's really a absolutely best choicefor Neuro D analysis.
Date published: 2017-07-29
Rated 5 out of 5 by from right bandI have used this antibody to do western blot on mouse brain tissue. I have incubated with this antibody (1:1000) at 4 °C over night, then with secondary antibody from CWBIO at 1:40000 and incubated at room temperature for 1 hour. The strong target band seen.
Date published: 2017-07-23
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Neuro D 抗体 (A-10) is rated 4.2 out of 5 by 16.
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