Date published: 2025-9-7
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Neurabin-II Antibody (D-7) - Western Blotting - Image 404192
Neurabin-II 抗体 (D-7) Alexa Fluor® 488: sc-373974 AF488. IMR-32 (A) 和 HEL 92. 1. 7 (B) 全细胞裂解液中 Neurabin-II 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.
Neurabin-II 抗体 (D-7) Alexa Fluor® 488: sc-373974 AF488. IMR-32 (A) 和 HEL 92. 1. 7 (B) 全细胞裂解液中 Neurabin-II 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.

Neurabin-II 抗体 (D-7): sc-373974

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寫評論提問

说明书
  • Neurabin-II 抗体 D-7 是小鼠单克隆 IgG1 κ，Neurabin-II抗体, 在2篇文献中引用，规格为200 µg/ml
  • 针对human起源的Neurabin-II内部区域内的氨基酸261-430进行
  • Neurabin-II 抗体 (D-7) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Neurabin-II
  • 抗Neurabin-II抗体（D-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Neurabin-II (D-7): sc-373974的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Neurabin-II Antibody (D-7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与Neurabin-II Antibody (D-7) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Neurabin抗体，包括Neurabin-I和Neurabin-II

Neurabin-II抗体（D-7）是一种IgG1 κ小鼠单克隆Neurabin-II抗体（也称为Neurabin-II抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人源的Neurabin-II蛋白。Neurabin-II抗体（D-7）既可以是非偶联的抗Neurabin-II抗体形式，也可以是多种偶联形式的抗Neurabin-II抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Neurabin-II，也称为spinophilin，在中枢神经系统的树突棘中与肌动蛋白和PP-1相互作用。编码人类neurabin-II的基因定位于17q21-q22染色体。Neurabin-II的结构特征包括N端区域的一个F-肌动蛋白结合域，C端的一个预测的卷曲螺旋结构，中间区域的一个PDZ域，以及一个已知与跨膜蛋白相互作用的域。Neurabin-II在体外可捆绑肌动蛋白细丝。在体内，spinophilin定位于肌动蛋白丝的皮质位点和活性膜重塑位点。Neurabin-II还与PP1的催化亚基形成复合物，并在体外调节PP1的酶活性。Neurabin-II定位于树突棘的头部，并辅助PP-1调节α-氨基-3-羟基-5-甲基-4-异噁唑丙酸（AMPA）和N-甲基-D-天冬氨酸（NMDA）受体的活性。通过这种方式，neurabin-II调节谷氨酸能突触传递和树突形态。Spinophilin与人类肿瘤抑制因子ARF之间的协同相互作用表明neurabin-II在细胞生长中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Neurabin-II 抗体 (D-7) 参考文献:

  1. l-afadin和neurabin-II在细胞-细胞粘连接头形成和破坏过程中的不同行为。  |  Sakisaka, T., et al. 1999. Oncogene. 18: 1609-17. PMID: 10102631
  2. F-肌动蛋白结合蛋白 neurabin-II 的体内动力学。  |  Stephens, DJ. and Banting, G. 2000. Biochem J. 345 Pt 2: 185-94. PMID: 10620493
  3. Spinophilin 调节树突棘的形成和功能。  |  Feng, J., et al. 2000. Proc Natl Acad Sci U S A. 97: 9287-92. PMID: 10922077
  4. 人类肿瘤抑制因子 arf 与 1 型蛋白磷酸酶结合蛋白 spinophilin/neurabin II 相互作用。  |  Vivo, M., et al. 2001. J Biol Chem. 276: 14161-9. PMID: 11278317
  5. 神经元肌动蛋白结合蛋白（neurabin I 和 neurabin II）会招募特定异构体的蛋白磷酸酶-1 催化亚基。  |  Terry-Lorenzo, RT., et al. 2002. J Biol Chem. 277: 27716-24. PMID: 12016225
  6. 鉴定 neurabin II 是一种新型双皮质素相互作用蛋白。  |  Tsukada, M., et al. 2003. Mech Dev. 120: 1033-43. PMID: 14550532
  7. 在 spinophilin/neurabin II 中发现第二个肌动蛋白结合区。  |  Barnes, AP., et al. 2004. Brain Res Mol Brain Res. 124: 105-13. PMID: 15135218
  8. 双皮质素与肌动蛋白丝的结合受神经原蛋白 II 的调节。  |  Tsukada, M., et al. 2005. J Biol Chem. 280: 11361-8. PMID: 15632197
  9. Neurabin II 在肌动蛋白丝上介导双皮质素磷酸化。  |  Tsukada, M., et al. 2006. Biochem Biophys Res Commun. 343: 839-47. PMID: 16564023
  10. 定位于树突棘的新型蛋白磷酸酶 1 结合蛋白 Spinophilin  |  Allen, PB., et al. 1997. Proc Natl Acad Sci U S A. 94: 9956-61. PMID: 9275233
  11. Neurabin-II/spinophilin.一种具有一个 pdz 结构域的肌动蛋白丝结合蛋白，定域于以干酪素为基础的细胞-细胞黏合剂位点。  |  Satoh, A., et al. 1998. J Biol Chem. 273: 3470-5. PMID: 9452470
  12. 大鼠 lin-10 与脑内丰富的 F-肌动蛋白结合蛋白、neurabin-II/spinophilin 的相互作用。  |  Ide, N., et al. 1998. Biochem Biophys Res Commun. 244: 258-62. PMID: 9514910

订购信息

产品名称产品编号规格价格数量收藏夹

Neurabin-II 抗体 (D-7)

sc-373974
200 µg/ml
RMB2377.00

Neurabin-II (D-7): m-IgG Fc BP-HRP 套装

sc-529624
200 µg Ab; 10 µg BP
RMB2662.00

Neurabin-II (D-7): m-IgGκ BP-HRP 套装

sc-522576
200 µg Ab, 40 µg BP
RMB2662.00

Neurabin-II 抗体 (D-7) AC

sc-373974 AC
500 µg/ml, 25% agarose
RMB3129.00

Neurabin-II 抗体 (D-7) HRP

sc-373974 HRP
200 µg/ml
RMB2377.00

Neurabin-II 抗体 (D-7) FITC

sc-373974 FITC
200 µg/ml
RMB2482.00

Neurabin-II 抗体 (D-7) PE

sc-373974 PE
200 µg/ml
RMB2580.00

Neurabin-II 抗体 (D-7) Alexa Fluor® 488

sc-373974 AF488
200 µg/ml
RMB2685.00

Neurabin-II 抗体 (D-7) Alexa Fluor® 546

sc-373974 AF546
200 µg/ml
RMB2685.00

Neurabin-II 抗体 (D-7) Alexa Fluor® 594

sc-373974 AF594
200 µg/ml
RMB2685.00

Neurabin-II 抗体 (D-7) Alexa Fluor® 647

sc-373974 AF647
200 µg/ml
RMB2685.00

Neurabin-II 抗体 (D-7) Alexa Fluor® 680

sc-373974 AF680
200 µg/ml
RMB2685.00

Neurabin-II 抗体 (D-7) Alexa Fluor® 790

sc-373974 AF790
200 µg/ml
RMB2685.00

Why does neurabin II run at ~130kD, when the sequence predicts an 89kDa protein? Seems like a large difference.

Asked by: mmoritz
Thank you for your question. The observed molecular weight is significantly higher than the predicted molecular weight. Please refer to the following publication as a reference for an approximately 140 kDa observed molecular weight for Neurabin-II, also known as Spinophilin, PMID: 9275233.
Answered by: Technical Support
Date published: 2019-11-22

I am using Neurabin-II (D-7): sc-373974 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from Great IF staining cortical neuronsStains cultured cortical neurons beautifully. Looks just like actin stain in the growth cone. First SPN antibody I have had that works. These are 36hr cultures of mouse cortical neurons. (Yes, it works on mouse) Use methanol fixation- (I do mine after PFA fixation) and the antibody at 1:100. Without methanol you can see a bit but its super faint.
Date published: 2017-12-08
Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-02-07
Rated 4 out of 5 by from Good for Western Blot in human brainWe have tested this antibody in human brain homogenates by Western Blot and we can visualise a clear band at 120 kDa approximately.
Date published: 2016-12-07
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and nuclearSuperb immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human salivary gland tissue. -SCBT QC
Date published: 2015-02-15
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Neurabin-II 抗体 (D-7) is rated 4.7 out of 5 by 6.
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