Date published: 2025-9-8

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nestin 抗体 (10c2): sc-23927

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说明书
  • nestin 抗体 10c2 是小鼠单克隆 IgG1 κ,nestin抗体, 在238篇文献中引用,规格为200 µg/ml
  • 针对human起源的nestin外显子C-terminus附近的150个氨基酸表位图谱
  • nestin 抗体 (10c2) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 nestin
  • 抗nestin抗体(10c2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取nestin (10c2): sc-23927免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 nestin 抗体 (10c2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与nestin 抗体 (10c2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Nestin抗体(10c2)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)检测小鼠、大鼠和人源的nestin蛋白。抗nestin抗体(10c2)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Nestin是一种重要的中间丝蛋白,主要存在于胚胎中枢神经系统的祖细胞中,在结构完整性和功能方面发挥着重要作用。在肌肉发育过程中,Nestin的表达至关重要,它与其他中间丝蛋白(如Desmin和Vimentin)相互作用,形成动态网络,支持细胞结构。Nestin能够与Vimentin或α-internexin共同组装成异二聚体螺旋线圈结构,从而形成10纳米的中间丝,对维持细胞形状和弹性至关重要。中间丝共识棒状结构域中的nestin缺失会破坏中枢神经系统前体细胞和放射状胶质细胞中的nestin定位,从而影响神经发育过程。Nestin在神经上皮干细胞、神经胶质瘤细胞和肿瘤内皮细胞快速生长期间充当标记物,凸显了nestin在正常发育和病理条件下的重要性。在神经元分化过程中,nestin定位到生长锥,表明其在引导轴突伸长方面发挥作用,这对于正常的神经网络形成至关重要。在大鼠肾上腺的束状带和网状带以及成肌早期阶段的皮层细胞和肌原细胞中观察到nestin表达,进一步强调了nestin在各种组织中的不同作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

nestin 抗体 (10c2) 参考文献:

  1. BHK-21 细胞中的一种高分子量中间丝相关蛋白是 nestin,它是一种 VI 型中间丝蛋白。在体外与 III 型波形蛋白和 IV 型 alpha-internexin 有限地共同组装,形成杂聚物。  |  Steinert, PM., et al. 1999. J Biol Chem. 274: 9881-90. PMID: 10092680
  2. 小鼠 nestin 蛋白定位于 P19 神经元和小脑颗粒细胞的生长锥。  |  Yan, Y., et al. 2001. Neurosci Lett. 302: 89-92. PMID: 11290394
  3. 作为胶质瘤增生内皮标志物的 Nestin。  |  Sugawara, K., et al. 2002. Lab Invest. 82: 345-51. PMID: 11896213
  4. 大鼠肾上腺中 Nestin 的表达  |  Bertelli, E., et al. 2002. Histochem Cell Biol. 117: 371-7. PMID: 11976910
  5. 中枢神经系统干细胞表达一种新型中间丝蛋白。  |  Lendahl, U., et al. 1990. Cell. 60: 585-95. PMID: 1689217
  6. 肌肉生成与中间丝蛋白--巢蛋白  |  Kachinsky, AM., et al. 1994. Dev Biol. 165: 216-28. PMID: 8088440
  7. 骨骼肌发育过程中中间丝 Nestin 的瞬时表达。  |  Sejersen, T. and Lendahl, U. 1993. J Cell Sci. 106 (Pt 4): 1291-300. PMID: 8126108
  8. 中间丝蛋白 nestin 的杆端缺失会改变其在转基因小鼠神经上皮细胞中的亚细胞定位。  |  Marvin, MJ., et al. 1998. J Cell Sci. 111 (Pt 14): 1951-61. PMID: 9645943

订购信息

产品名称产品编号规格价格数量收藏夹

nestin 抗体 (10c2)

sc-23927
200 µg/ml
RMB2377.00

nestin (10c2): m-IgG Fc BP-HRP 套装

sc-526636
200 µg Ab; 10 µg BP
RMB2662.00

nestin (10c2): m-IgG1 BP-HRP 套装

sc-532009
200 µg Ab; 20 µg BP
RMB2662.00

nestin 抗体 (10c2) AC

sc-23927 AC
500 µg/ml, 25% agarose
RMB3129.00

nestin 抗体 (10c2) HRP

sc-23927 HRP
200 µg/ml
RMB2377.00

nestin 抗体 (10c2) FITC

sc-23927 FITC
200 µg/ml
RMB2482.00

nestin 抗体 (10c2) PE

sc-23927 PE
200 µg/ml
RMB2580.00

nestin 抗体 (10c2) Alexa Fluor® 488

sc-23927 AF488
200 µg/ml
RMB2685.00

nestin 抗体 (10c2) Alexa Fluor® 546

sc-23927 AF546
200 µg/ml
RMB2685.00

nestin 抗体 (10c2) Alexa Fluor® 594

sc-23927 AF594
200 µg/ml
RMB2685.00

nestin 抗体 (10c2) Alexa Fluor® 647

sc-23927 AF647
200 µg/ml
RMB2685.00

nestin 抗体 (10c2) Alexa Fluor® 680

sc-23927 AF680
200 µg/ml
RMB2685.00

nestin 抗体 (10c2) Alexa Fluor® 790

sc-23927 AF790
200 µg/ml
RMB2685.00

What is the desired Antigen Retrieval method recommended for Nestin Antibody?

Asked by: DishaV
Thank you for your question. We recommend sodium citrate with heat at pH 6 for antigen retrieval when using paraffin embedded tissue sections by IHC.
Answered by: Santa Cruz TS
Date published: 2021-07-05

I want evidence regarding Nestin in adult rodent amygdala if there's any image or reference for the same since I couldn't find anything related to that.

Asked by: Karishma
Thank you for your question. We have immunohistochemistry data for nestin in mouse cerebral cortex. We do not have any data or product citations specifically in amygdala for our nestin antibodies. This would have to be determined experimentally.
Answered by: Technical Support
Date published: 2020-05-11

Hi, I had a question regarding the unit size of one of your antibodies: sc-23927. Specifically, I was hoping to find out how much ug or mL I would be getting from one order of this product? Thanks! Eric

Asked by: turdyturtle
Thank you for your question. This antibody, nestin (10c2): sc-23927, is provided as 200 µg in 1 mL.
Answered by: Technical Support
Date published: 2017-07-26

I am setting up a new lab and shopping for a bunch of antibodies. I am looking for an alternative for discontinued Nestin (goat). Your nestin Antibody (10c2): sc-23927 looks promsing. Would you please providing any sample? This help us choose a ventor.

Asked by: Michael1
Thank you for your question. For a limited time, we are currently offering free 50µg samples of our mouse monoclonal antibodies, including, nestin (10c2): sc-23927. This antibody has been validated to work in WB, IP and IHC(P) with mouse, rat and human samples. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, email [email protected], or use the live chat on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2018-11-08

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-22
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Rated 5 out of 5 by from Good antibody for human cellsI used this antibody to detect a brain tumor in a mouse brain after transplantation. I recommend this antibody for detecting Nestin in human cells.
Date published: 2024-09-03
Rated 5 out of 5 by from IHCで使用できるIHCで綺麗にシグナルが出ました。PMID: 37542080、PMID: 36524054にて発表済み。
Date published: 2024-02-16
Rated 4 out of 5 by from Nice specificity in IFOttimo per neural stem cells e brain tumour stem cells
Date published: 2019-04-09
Rated 4 out of 5 by from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from It works good in ONPC WBWe tested in western blot, 40ug protein, 7% de Acrylamide (1:200) and it worked good in our samples of olfactory neuroepitellium cells (human).
Date published: 2018-10-01
Rated 5 out of 5 by from Sehr Gute ErgebnisseSehr gute Ergebnisse bei Western Blot und auch bei Immunoprecipitation.
Date published: 2018-09-21
Rated 5 out of 5 by from Great antibody for IHCWe used this antibody on NPCs, and it worked great.
Date published: 2018-07-19
Rated 3 out of 5 by from Works OKThis antibody stains fair in neuronal cell derived from human somatic cells.
Date published: 2017-12-21
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nestin 抗体 (10c2) is rated 4.7 out of 5 by 29.
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