Date published: 2025-9-8

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nephrin 抗体 (G-8): sc-376522

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说明书
  • nephrin 抗体 G-8 是小鼠单克隆 IgG2a κ,nephrin抗体, 在30篇文献中引用,规格为200 µg/ml
  • 在human起源的nephrin细胞外结构域内与氨基酸23-322的映射
  • nephrin 抗体 (G-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 nephrin
  • 抗nephrin抗体(G-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体,可用于WB、IHC(P)和ELISA实验
  • 关于如何获取nephrin (G-8): sc-376522免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是nephrin Antibody (G-8) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与nephrin Antibody (G-8) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

nephrin抗体(G-8)是一种IgG2a κ小鼠单克隆nephrin抗体(也被称为NPHS1抗体或粘附分子nephrin抗体),能够通过WB, IP, IF和ELISA检测小鼠、大鼠和人的nephrin蛋白。nephrin抗体(G-8)既可以作为非偶联的抗nephrin抗体形式,也可以作为多种偶联形式的抗nephrin抗体,包括琼脂糖、biotin、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。Nephrin是免疫球蛋白家族细胞粘附分子的成员,它定位于由足细胞形成的次级足突的相对位置,足细胞是一种特殊的上皮细胞,确保大小和电荷选择性的超滤。人类nephrin基因定位于染色体19q13.1,编码一个包含跨膜结构域、八个Ig样模块和一个纤连蛋白III样模块的1241个氨基酸的蛋白质。Nephrin在胚胎和成人肾脏中表达,并定位于肾小球足细胞和肾小球裂孔隔膜。Nephrin刺激丝裂原活化蛋白激酶,并被podocin增强,podocin与nephrin的细胞质尾部结合。用佛波醇-12-肉豆蔻酸酯-13-乙酸酯处理的A293细胞可以上调nephrin,这表明蛋白激酶C是调节nephrin的细胞内信号系统的一部分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

nephrin 抗体 (G-8)

sc-376522
200 µg/ml
RMB2377.00

nephrin (G-8): m-IgGκ BP-HRP 套装

sc-522989
200 µg Ab, 40 µg BP
RMB2662.00

nephrin 抗体 (G-8) AC

sc-376522 AC
500 µg/ml, 25% agarose
RMB3129.00

nephrin 抗体 (G-8) HRP

sc-376522 HRP
200 µg/ml
RMB2377.00

nephrin 抗体 (G-8) FITC

sc-376522 FITC
200 µg/ml
RMB2482.00

nephrin 抗体 (G-8) PE

sc-376522 PE
200 µg/ml
RMB2580.00

nephrin 抗体 (G-8) Alexa Fluor® 488

sc-376522 AF488
200 µg/ml
RMB2685.00

nephrin 抗体 (G-8) Alexa Fluor® 546

sc-376522 AF546
200 µg/ml
RMB2685.00

nephrin 抗体 (G-8) Alexa Fluor® 594

sc-376522 AF594
200 µg/ml
RMB2685.00

nephrin 抗体 (G-8) Alexa Fluor® 647

sc-376522 AF647
200 µg/ml
RMB2685.00

nephrin 抗体 (G-8) Alexa Fluor® 680

sc-376522 AF680
200 µg/ml
RMB2685.00

nephrin 抗体 (G-8) Alexa Fluor® 790

sc-376522 AF790
200 µg/ml
RMB2685.00

nephrin 抗体 (G-8) B

sc-376522 B
200 µg/ml
RMB2407.00

Does this antibody work in immunohistochemistry?

Asked by: nasim
Thank you for your question. Anti-nephrin Antibody (G-8) is recommended for detection of nephrin of mouse, rat and human origin by WB, IP, IF and ELISA. We do not recommend it for IHC or IHC(P) applications.
Answered by: Tech Support Europe
Date published: 2022-08-03

Does it work for FACS sorting?

Asked by: slu68
Thank you for your question. Nephrin Antibody (G-8): sc-376522 is only recommended for detection of nephrin of mouse, rat and human origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2020-08-25

Can we use anti-nephrin Antibody (G-8): sc-376522 for IHC on formalin-fixed rat kidneys?

Asked by: Kuldeep
Thank you for your question. This antibody is not currently recommended for IHC on formalin-fixed paraffin-embedded tissue, or for IHC(P). Please check back for updates!
Answered by: Technical Support
Date published: 2021-01-09

Can this antibody be used for Formalin-Fixed Paraffin Embedded tissue sections from mice?

Asked by: kuldrpa1
The conjugated primary antibody is recommended and warrantied for IHC(P), however we are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-01-22

Can nephrin (G-8): sc-376522 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, nephrin (G-8): sc-376522 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Did not work wellThis Ab did not work well for neonatal mouse lungs for WB
Date published: 2024-12-13
Rated 4 out of 5 by from 蛍光抗体法で使用しましたサンプル品を蛍光抗体法で使用しました。マウス腎臓由来の株細胞で微弱ながらもシグナルがみられました。また、ある膜タンパク質の発現変動によって、蛍光抗体法のシグナル強度が変化する様子を観察することもできました。
Date published: 2024-10-02
Rated 5 out of 5 by from Great for WBThis antibody works great for detection of nephrin in rat kidney by WB.
Date published: 2020-04-08
Rated 5 out of 5 by from Nice Western Blot data of nephrin expressionNice Western Blot data of nephrin expression in human kidney tissue extract. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Satisfactory Western Blot data of nephrinSatisfactory Western Blot data of nephrin expression in rat kidney tissue extract. -SCBT QC
Date published: 2013-04-25
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nephrin 抗体 (G-8) is rated 4.0 out of 5 by 5.
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