Date published: 2025-9-8
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NCAM/CD56 Antibody (123C3) - Western Blotting - Image 379848
NCAM 抗体 (123C3): sc-7326. IMR-32 (A) 和 U-87 MG (B) 全细胞裂解液中 NCAM 表达的 Western 印迹分析.
NCAM 抗体 (123C3): sc-7326. IMR-32 (A) 和 U-87 MG (B) 全细胞裂解液中 NCAM 表达的 Western 印迹分析.

NCAM/CD56 抗体 (123C3): sc-7326

4.1(22)
寫評論提問

说明书
  • NCAM/CD56 抗体 123C3 是小鼠单克隆 IgG1 κ，NCAM/CD56抗体, 在61篇文献中引用，规格为200 µg/ml
  • 针对human起源的NCAM的膜制备
  • NCAM/CD56 抗体 (123C3) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 NCAM; 还建议用于检测neuroectodermally derived tumors such as neuroblastoma, medulloblastoma, astrocytoma and retinoblastoma
  • 抗NCAM/CD56抗体（123C3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NCAM/CD56 (123C3): sc-7326的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 NCAM/CD56 抗体 (123C3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NCAM/CD56 抗体 (123C3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 NCAM 抗体包括 NCAM-L1 和 NCAM2

神经细胞粘附分子抗体（123C3）是一种小鼠单克隆IgG1 kappa轻链抗体，专门针对神经细胞粘附分子1（NCAM1），也称为CD56，是一种位于神经细胞质膜上的重要细胞表面糖蛋白。这种策略性的定位使NCAM1能够在胚胎发育过程中促进细胞间的重要相互作用，并维持成人组织中神经网络的完整性。抗NCAM1抗体（123C3）可有效检测小鼠、大鼠和人类来源的NCAM1蛋白，适用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）。特别推荐使用NCAM1单克隆抗体（123C3）来识别神经外胚层来源的肿瘤，如神经母细胞瘤、髓母细胞瘤、星形细胞瘤和视网膜母细胞瘤，这凸显了NCAM1在肿瘤生物学中的重要性。NCAM1 (123C3)抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和Alexa Fluor®变体，为研究细胞粘附、神经发育和相关病理的研究人员提供了多种选择。此外，抗NCAM1抗体（123C3）可识别由可变剪接和多聚腺苷酸化产生的多种NCAM1异构体，从而提高其在各种实验模型中的实用性。无论是用于基础研究还是临床诊断，NCAM1（123C3）单克隆抗体都是增进我们对神经细胞相互作用和相关疾病理解不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NCAM/CD56 抗体 (123C3) 参考文献:

  1. NCAM模拟肽：药理和治疗潜力。  |  Berezin, V. and Bock, E. 2004. J Mol Neurosci. 22: 33-39. PMID: 14742908
  2. 拉链发出信号：NCAM介导的分子相互作用和信号转导。  |  Walmod, PS., et al. 2004. Neurochem Res. 29: 2015-35. PMID: 15662836
  3. 神经细胞粘附分子（NCAM）同源结合和FGFR活化的结构生物学。  |  Kiselyov, VV., et al. 2005. J Neurochem. 94: 1169-79. PMID: 16045455
  4. 重新审视NCAM诱导的细胞内信号传导。  |  Ditlevsen, DK., et al. 2008. J Neurosci Res. 86: 727-43. PMID: 17975827
  5. NCAM和FGF受体。  |  Kiselyov, VV. 2010. Adv Exp Med Biol. 663: 67-79. PMID: 20017015
  6. NCAM的生物合成。  |  Kolkova, K. 2010. Adv Exp Med Biol. 663: 213-25. PMID: 20017025
  7. 长期增强和学习中的NCAM。  |  Hartz, BP. and Rønn, LC. 2010. Adv Exp Med Biol. 663: 257-70. PMID: 20017028
  8. 神经细胞粘附分子（NCAM）多糖化与细胞迁移的分子内和分子间相互作用的可能调节机制。  |  Lu, B., et al. 2019. Curr Top Med Chem. 19: 2271-2282. PMID: 31648641
  9. 普遍存在的神经细胞粘附分子（NCAM）：癌症病理生理学、药物靶向和分子转导的潜在机制和价值。  |  Sowparani, S., et al. 2022. Mol Neurobiol. 59: 5902-5924. PMID: 35831555
  10. CD56/NCAM通过促进整合素介导的粘附更新来介导人类NK细胞的迁移。  |  Martinez, AL., et al. 2024. Mol Biol Cell. 35: ar64. PMID: 38507235

订购信息

产品名称产品编号规格价格数量收藏夹

NCAM/CD56 抗体 (123C3)

sc-7326
200 µg/ml
RMB2377.00

NCAM/CD56 (123C3): m-IgG Fc BP-HRP 套装

sc-528175
200 µg Ab; 10 µg BP
RMB2662.00

NCAM/CD56 (123C3): m-IgGκ BP-HRP 套装

sc-520492
200 µg Ab, 40 µg BP
RMB2662.00

NCAM/CD56 (123C3): m-IgG1 BP-HRP 套装

sc-542801
200 µg Ab; 20 µg BP
RMB2662.00

NCAM/CD56 抗体 (123C3) AC

sc-7326 AC
500 µg/ml, 25% agarose
RMB3129.00

NCAM/CD56 抗体 (123C3) HRP

sc-7326 HRP
200 µg/ml
RMB2377.00

NCAM/CD56 抗体 (123C3) FITC

sc-7326 FITC
200 µg/ml
RMB2482.00

NCAM/CD56 抗体 (123C3) PE

sc-7326 PE
200 µg/ml
RMB2580.00

NCAM/CD56 抗体 (123C3) Alexa Fluor® 488

sc-7326 AF488
200 µg/ml
RMB2685.00

NCAM/CD56 抗体 (123C3) Alexa Fluor® 546

sc-7326 AF546
200 µg/ml
RMB2685.00

NCAM/CD56 抗体 (123C3) Alexa Fluor® 594

sc-7326 AF594
200 µg/ml
RMB2685.00

NCAM/CD56 抗体 (123C3) Alexa Fluor® 647

sc-7326 AF647
200 µg/ml
RMB2685.00

NCAM/CD56 抗体 (123C3) Alexa Fluor® 680

sc-7326 AF680
200 µg/ml
RMB2685.00

NCAM/CD56 抗体 (123C3) Alexa Fluor® 790

sc-7326 AF790
200 µg/ml
RMB2685.00

这产品是含偶联物一起发的吗，我搭配二抗还要单独买偶联物吗

Asked by: wlzm
感谢您的提问。NCAM/CD56 抗体 (123C3): sc-7326不含偶联物。您可以购买标记了偶联物的一抗，或者未偶联的一抗并搭配二抗使用，二抗上面标记了偶联物。 Thank for your question. NCAM/CD56 antibody (123C3): sc-7326 doesn't contain conjugation. You can buy conjugated primary antibody, or unconjugated primary antibody and work with a conjugated secondary antibody.
Answered by: Technical Service SH
Date published: 2022-03-10

本公司推荐的二抗跟这个一抗搭配做免疫荧光或者组化的话，还需要单独买偶联物嘛？

Asked by: wlzm
感谢您的提问。不需要单独购买偶联物。二抗上面有偶联物。 Thank you for your question. You don't need to buy a separate conjugation, secondary antibody contains the conjugation.
Answered by: Technical Service SH
Date published: 2022-03-10

可以用于冰冻切片的免疫组化SP法吗

Asked by: wlzm
感谢您的提问。NCAM/CD56 抗体 (123C3): sc-7326 可以用于小鼠、大鼠、人源样本冰冻切片的免疫组化SP法。 Thank you for your question. NCAM/CD56 antibody (123C3): sc-7326 can be used to detect mouse, rat, and human origin frozen tissue sections by IHC (SP method).
Answered by: Technical Service SH
Date published: 2022-03-10

想问一下免疫组化或者荧光的话只能与偶联物偶联使用吗还是单独使用也可以 还有保存的话负20℃冰箱就可以吗

Asked by: wlzm
感谢您的提问。您可以选择含有偶联物的一抗，或者未偶联的一抗搭配偶联的二抗，进行免疫组化或者荧光实验。在4°C保存抗体，不可以冷冻。 Thank you for your question. You can use conjugated primary antibody, or unconjugated primary antibody working with conjugated secondary antibody for IF or IHC. This antibody should be stored at 4°C, do not freeze.
Answered by: Technical Service SH
Date published: 2022-03-09

Hi, it is available conjugated to biotin?

Asked by: Anonymous
Thank you for your question. Please contact our technical service team so we can arrange a special order for the biotin-conjugated version of this antibody with you.
Answered by: Tech Support Europe
Date published: 2020-06-25

How large are the agarose beads that are attached to the antibody?

Asked by: alison26
Thank you for your question. The average (pore size) of the agarose is 90 microns (um). We use beaded agarose type 4B. Our primary antibodies are covalently bound to the agarose beads via primary amines. For further information, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-09-17

Can I use NCAM (123C3): sc-7326 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jenni
Yes. We recommend using a directly conjugated monoclonal antibody. NCAM (123C3): sc-7326 monoclonal antibody is available directly conjugated to either HRP (sc-7326 HRP), phycoerythrin (sc-7326 PE), fluorescein (sc-7326 FITC), Alexa Fluor® 488 (sc-7326 AF488) or Alexa Fluor® 647 (sc-7326 AF647)
Answered by: Technical Support 3
Date published: 2020-09-12
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Rated 4 out of 5 by from Works for western blottingI used this antibody for western blotting application with this diluition conditions: blocking in 5% MILK for 1h; primary antibody 1:1000 in 3% BSA over night; secondary antibody 1:2000 3% MILK for 1h. The signal of all isoforms clearly appear after 15/20 minutes of exposition.
Date published: 2021-12-14
Rated 4 out of 5 by from Works for flow cytometryThe NCAM Antibody (123C3) works for flow cytometry. NCAM Antibody (ERIC 1) - sc-106 seemed to give slightly better staining.
Date published: 2018-10-25
Rated 4 out of 5 by from Affidabile!Ho testato questo Ab in western blotting e ne ho constatato la buona reattività e specificità.
Date published: 2017-11-16
Rated 1 out of 5 by from No visible stainingTried the sample out for ICC and did not see any positive results. Tried at 1:200 and 1:500
Date published: 2017-11-02
Rated 2 out of 5 by from é un buon anticorpoHo ricevuto un campione omaggio, e penso che in futuro acquisterò il prodotto
Date published: 2017-10-13
Rated 1 out of 5 by from Not working for mouse brain immunofluorescenceI got a sample of this one a month ago and tested on mouse brain free floating sections. Under all the dilutions that I tried (1:50, 1:200, 1:800), I did not see any positive staining
Date published: 2017-09-14
Rated 5 out of 5 by from NCAM ANTIBODYIt is available for WB by dulution at 1:500 in tm4 sertoli cell lines.
Date published: 2017-08-31
Rated 5 out of 5 by from Excellent work in ImmunohistochemistryI used this NCAM antibody (123C3) for my immunohistochemistry experiment of tumor tissues in mice. The CD56+ cells were well identified and the results were great.
Date published: 2017-02-15
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NCAM/CD56 抗体 (123C3) is rated 4.1 out of 5 by 22.
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