Date published: 2025-9-8

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NCAM/CD56 抗体 (123A8): sc-59864

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说明书
  • NCAM/CD56抗体(123A8)是小鼠单克隆IgG1 κ, 在2篇文献中引用,规格为200 µg/ml
  • 免疫human物种的NCAM
  • 推荐用于 mouse, rat 和 human 来源的NCAM WB, IP, IF, IHC(P) 和 FCM检测; 还建议用于检测neuroectodermally derived tumors such as neuroblastoma, medulloblastoma, astrocytoma and retinoblastoma
  • 看 NCAM/CD56 (123C3): sc-7326,可查看有标记的 NCAM/CD56 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 NCAM/CD56 抗体 (123A8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NCAM/CD56 抗体 (123A8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

NCAM抗体(123A8)是一种小鼠单克隆IgG1κ轻链抗体,专门用于靶向人类神经细胞粘附分子(NCAM),也称为CD56或NCAM1。NCAM是一种重要的细胞表面糖蛋白,可促进神经发育、突触可塑性和胚胎发育所必需的细胞间相互作用,在神经系统的形成和维护中起着至关重要的作用。NCAM单克隆抗体(123A8)可有效检测多种物种(包括小鼠、大鼠和人类)的NCAM,推荐用于蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)。除了在正常生理研究中的应用,NCAM(123A8)单克隆抗体还强烈推荐用于识别和研究神经外胚层来源的肿瘤,如神经母细胞瘤、髓母细胞瘤、星形细胞瘤和视网膜母细胞瘤,在这些肿瘤中,NCAM的表达非常明显。NCAM存在于多种异构体中,这些异构体是通过位于人类11q23染色体上的单个基因的替代剪接和多聚腺苷酸化产生的,这有助于其在健康和疾病状态下的多种功能。抗NCAM抗体(123A8)有助于研究人员阐明细胞粘附、神经网络形成和各种神经相关癌症进展的分子机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NCAM/CD56 抗体 (123A8) 参考文献:

  1. 神经细胞粘附分子介导的细胞外蛋白相互作用:神经细胞粘附分子与细胞粘附分子、细胞外基质蛋白和病毒之间的异相相互作用。  |  Nielsen, J., et al. 2010. Adv Exp Med Biol. 663: 23-53. PMID: 20017013
  2. NCAM在听觉恐惧条件反射中的作用及其受压力的调节:聚焦杏仁核。  |  Bisaz, R. and Sandi, C. 2010. Genes Brain Behav. 9: 353-64. PMID: 20059553
  3. NCAM/pectrin复合物的解体会导致PSD穿孔和突触后内细胞区的形成。  |  Puchkov, D., et al. 2011. Cereb Cortex. 21: 2217-32. PMID: 21339376
  4. 小鼠前脑中NCAM的缺乏会损害先天性和后天性回避行为。  |  Brandewiede, J., et al. 2014. Genes Brain Behav. 13: 468-77. PMID: 24751161
  5. [聚唾液酸(PSA)和聚唾液酸神经细胞粘附分子(PSA-NCAM)对肿瘤和细胞信号传导通路的影响]。  |  Wang, X. and Guan, F. 2014. Yi Chuan. 36: 739-46. PMID: 25143271
  6. 神经元周围网状蛋白 Neurocan 可抑制 GABA 能神经元中的 NCAM/EphA3 排斥信号传导  |  Sullivan, CS., et al. 2018. Sci Rep. 8: 6143. PMID: 29670169
  7. 原生化合物偶联亲和基质(NCAM)在生物活性化合物靶标鉴定和验证中的应用:应用、机制与展望。  |  Ma, L., et al. 2023. Bioorg Chem. 140: 106828. PMID: 37690368
  8. 鸡原发性心房间隔心内膜细胞上的多唾液酸神经细胞粘附分子表达。  |  Gerety, M. and Watanabe, M. 1997. Anat Rec. 247: 71-84. PMID: 8986305
  9. 神经细胞粘附分子对于海马神经轴突生长和束状化至关重要。  |  Cremer, H., et al. 1997. Mol Cell Neurosci. 8: 323-35. PMID: 9073395
  10. NCAM与精神分裂症:遗传学研究。  |  Vicente, AM., et al. 1997. Mol Psychiatry. 2: 65-9. PMID: 9154219

订购信息

产品名称产品编号规格价格数量收藏夹

NCAM/CD56 抗体 (123A8)

sc-59864
200 µg/ml
RMB2377.00

NCAM/CD56 (123A8): m-IgG Fc BP-HRP 套装

sc-539145
200 µg Ab; 10 µg BP
RMB2662.00

NCAM/CD56 (123A8): m-IgG1 BP-HRP 套装

sc-541292
200 µg Ab; 20 µg BP
RMB2662.00

可以用于冰冻切片的免疫组化吗?可以用于豚鼠吗?

Asked by: wlzm
感谢您的提问。NCAM/CD56 抗体 (123A8): sc-59864可以用于冰冻切片的免疫组化,但是在豚鼠上没有测试过。Thank you for your question. NCAM/CD56 antibody (123A8): sc-59864 can be used on frozen tissue slides on IHC, but we didn't test it on guinea pig samples.
Answered by: Technical Service SH
Date published: 2022-02-23

how much is volume of NCAM Antibody (123A8): sc-59864 ihc kit? how many staining of medium size specimen  is possible?

Asked by: jaafari
Thank you very much for your inquiry. The NCAM Antibody (123A8): sc-59864 provided at 200 µg in 1 ml buffer. We would recommend to use a starting dilution of 1:50 for IHC(P). The number of slides that you can stain with one vial depends on the volume you are using for incubating each slide. Please find further information in our protocols under the following link: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-02

Can NCAM (123A8): sc-59864 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, NCAM (123A8): sc-59864 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Worked with nonWorked with non-transfected and human NCAM transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-10
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal strength in SHP-77 whole cell lysate. -SCBT QC
Date published: 2013-07-16
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NCAM/CD56 抗体 (123A8) is rated 4.0 out of 5 by 2.
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