Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
N-Ras Antibody (F155) - Western Blotting - Image 4197
N-Ras 抗体 (F155): sc-31. HeLa (A), A-431 (B), Jurkat (C) 和 NIH/3T3 (D) 全细胞裂解液中 N-Ras 表达的 Western 印迹分析.
N-Ras 抗体 (F155): sc-31. HeLa (A), A-431 (B), Jurkat (C) 和 NIH/3T3 (D) 全细胞裂解液中 N-Ras 表达的 Western 印迹分析.

N-Ras 抗体 (F155): sc-31

4.8(13)
寫評論提問

说明书
  • 抗-N-Ras抗体(F155)是小鼠单克隆IgG1 κ N-Ras抗体, 在240篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组N-Ras p21
  • 推荐用于 mouse, rat 和 human 来源的N-Ras p21 WB, IP, IF 和 IHC(P)检测
  • 抗N-Ras抗体（F155）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取N-Ras (F155): sc-31的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 N-Ras 抗体 (F155) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与N-Ras 抗体 (F155) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Ras 抗体 包括 Ras-GRF1, κ B-Ras1, κ B-Ras2, E-Ras, H-Ras, R-Ras, pan Ras, Ras GAP, Ras2和K-Ras

N-Ras抗体（F155）是一种IgG1小鼠单克隆N-Ras抗体（也称为NRAS抗体），可通过WB、IP、IF和IHC（P）检测来自小鼠、大鼠和人类的N-Ras蛋白。N-Ras抗体（F155）可以作为非偶联的抗N-Ras抗体使用。哺乳动物Ras（也称为v-Ha-Ras、Harvey大鼠肉瘤病毒致癌基因同源物、HRAS1、K-Ras、N-Ras、RASH1或c-bas/has）基因家族由Harvey和Kirsten Ras基因（c-H-Ras1和c-K-Ras2）、每个基因的一个无活性假基因（c-H-Ras2和c-K-Ras1）以及N-Ras基因组成。三种Ras致癌基因H-Ras、K-Ras和N-Ras编码具有GTP/GDP结合和GTP酶活性的蛋白质。Ras蛋白在GDP结合的无活性形式和GTP结合的活性形式之间交替被环氧核苷酸交换因子（GEF）激活，被GTP酶激活蛋白（GAP）失活。Ras的命名来源于与C型哺乳动物逆转录病毒Harvey株鼠类肉瘤病毒(HaMSV)致癌序列的克隆DNA片段同源的人类DNA序列的特征。在正常情况下，Ras家族成员通过亚细胞膜分离的信号传导系统影响细胞生长和分化。致癌Ras可以解除控制细胞增殖和凋亡的过程。GTP水解耦合信号转导中继蛋白Ras超家族可以分为Ras、Rho、Rab和ARF家族。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

N-Ras 抗体 (F155) 参考文献:

  1. Ras 的阴暗面：细胞凋亡的调控。  |  Cox, AD. and Der, CJ. 2003. Oncogene. 22: 8999-9006. PMID: 14663478
  2. 前列腺癌进展过程中的 Ras 信号传导。  |  Weber, MJ. and Gioeli, D. 2004. J Cell Biochem. 91: 13-25. PMID: 14689577
  3. 人类 RAS 超家族蛋白和相关 GTP 酶。  |  Colicelli, J. 2004. Sci STKE. 2004: RE13. PMID: 15367757
  4. 肿瘤进展和转移中的致癌 Ras。  |  Giehl, K. 2005. Biol Chem. 386: 193-205. PMID: 15843165
  5. Ras 质膜信号平台。  |  Hancock, JF. and Parton, RG. 2005. Biochem J. 389: 1-11. PMID: 15954863
  6. 高尔基体上的 Ras 信号传导。  |  Quatela, SE. and Philips, MR. 2006. Curr Opin Cell Biol. 18: 162-7. PMID: 16488589
  7. 分区化的 Ras/MAPK 信号传导。  |  Mor, A. and Philips, MR. 2006. Annu Rev Immunol. 24: 771-800. PMID: 16551266
  8. Ras, PI(3)K 和 mTOR 信号控制着肿瘤细胞的生长。  |  Shaw, RJ. and Cantley, LC. 2006. Nature. 441: 424-30. PMID: 16724053
  9. 人类 DNA 中与哺乳动物肉瘤逆转录病毒转化基因相关的三个不同基因。  |  Wong-Staal, F., et al. 1981. Science. 213: 226-8. PMID: 6264598

订购信息

产品名称产品编号规格价格数量收藏夹

N-Ras 抗体 (F155)

sc-31
100 µg/ml
RMB2377.00

N-Ras (F155): m-IgG Fc BP-HRP 套装

sc-551590
100 µg Ab; 10 µg BP
RMB2662.00

N-Ras (F155): m-IgG1 BP-HRP 套装

sc-551589
100 µg Ab; 20 µg BP
RMB2662.00

N-Ras (F155): m-IgGκ BP-HRP 套装

sc-551591
100 µg Ab; 40 µg BP
RMB2662.00

N-Ras 抗体 (F155) AC

sc-31 AC
500 µg/ml, 25% agarose
RMB3129.00

N-Ras 抗体 (F155) HRP

sc-31 HRP
200 µg/ml
RMB2377.00

N-Ras 抗体 (F155) FITC

sc-31 FITC
200 µg/ml
RMB2482.00

N-Ras 抗体 (F155) PE

sc-31 PE
200 µg/ml
RMB2580.00

N-Ras 抗体 (F155) Alexa Fluor® 488

sc-31 AF488
200 µg/ml
RMB2685.00

N-Ras 抗体 (F155) Alexa Fluor® 546

sc-31 AF546
200 µg/ml
RMB2685.00

N-Ras 抗体 (F155) Alexa Fluor® 594

sc-31 AF594
200 µg/ml
RMB2685.00

N-Ras 抗体 (F155) Alexa Fluor® 647

sc-31 AF647
200 µg/ml
RMB2685.00

N-Ras 抗体 (F155) Alexa Fluor® 680

sc-31 AF680
200 µg/ml
RMB2685.00

N-Ras 抗体 (F155) Alexa Fluor® 790

sc-31 AF790
200 µg/ml
RMB2685.00

Hi - I see a question from 7 years ago asking about the immunogen peptide sequence for this antibody with the reply being that it's unknown. Is that still the case, or if it's known now, could you share what the immunogen peptide sequence is? Thank you.

Asked by: Stephanie
Thank you for your question . The sequence is proprietary.
Answered by: BlakeJ
Date published: 2024-06-26

I have been use your NRAS antibody sc-31 , but i heard from my co-work said he tried to order this antibody but wait for a long time then he has been told it is discontinued. I want to order two more, let me know

Asked by: Hong Xiao
Thank you for your question. For accurate availability, shipping and pricing information regarding our antibodies, or to receive a quote, please contact Customer Service.
Answered by: Technical Service
Date published: 2024-03-28

Hi, can this N-Ras antibody(F155) be used to detect the Nras encoded by mutated NRAS, such as NrasQ61R?

Asked by: sssylvana
Thank you for your question. This antibody is raised against recombinant N-Ras p21 of human origin, recommended for detection of N-Ras p21 of mouse, rat and human origin by WB, IP, IF and IHC(P), we didn't test it on mutated NRAS.
Answered by: Technical Support 12
Date published: 2024-06-24

Hello, I am interested in "N-Ras Antibody (F155): sc-31" for Western blot use. What were the duration, buffers, Ab concentrations, and temperature/conditions (for blocking/probing) used to QC this Ab? Thank you in advance

Asked by: Anonymous
Thank you for your question. If you are using ECL for detection, we recommend blocking (PVDF membrane) in 5% nonfat milk + TBST for 1 hour at room temperature. We recommend using the same buffer (freshly prepared) as a diluent for both primary and secondary antibodies. If you are using Near-Infrared (NIR) Detection methods, then we recommend using our UltraCruz Blocking Reagent: sc-516214 instead of milk or BSA to reduce background. Recommended starting dilution for Western blot for the primary antibody is 1:200 (range 1:100-1:1,000). We recommend incubating primary antibody for 1 hour at room temperature, or overnight at 4C. Please refer to our recommended protocol online (or use the link below) for step-by-step detailed methods for how this antibody was tested for the data shown on our website. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2020-05-07

Your data sheet says" N-Ras (F155) is a mouse monoclonal antibody raised against recombinant p21 protein" Is it ok to tell what the immunogen peptide sequence is? Thanks

Asked by: Zhi123456789
Thank you for your question. The exact recombinant protein p21 immunogen sequence used to develop this mouse monoclonal antibody, N-Ras (F155): sc-31, is unknown.
Answered by: Technical Support
Date published: 2017-09-11

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
No. Since no direct conjugates are available for this antibody, we would not recommend using it for IHC in mouse tissue. See pan Ras (C-4): sc-166691 for N-Ras antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Tech Service 5
Date published: 2016-12-16

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_7
  • loc_zh_CN, sid_31, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 101ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from N-Ras sc-31 for western blotVery good for western blotting (Hela cells). Detects a specific band at ~ 16 kDa.
Date published: 2024-02-10
Rated 4 out of 5 by from Application to ICCI have tested this Ab for the immunocytochemical detection of NRAS overexpressed in a thyroid-derived cell line. SIgnals were detected from cells transfected with the NRAS expression plasmid, although their intensity was low. No signals were observed in the non-transfected cell lines; therefore, detection of endogenous NRAS may be difficult.
Date published: 2023-02-21
Rated 4 out of 5 by from Specific for NRAS on WesternThis antibody specifically sees NRAS and does not crossreact with KRAS or HRAS. We use to confirm CRISPR of NRAS at 1:100. Leave primary on for 4 days for best results.
Date published: 2021-02-16
Rated 5 out of 5 by from NRASAplin lab used NRAS, SC-31 antibody for western blotting.
Date published: 2019-02-06
Rated 5 out of 5 by from Excellent AntibodyI tested this antibody on mouse brain lysate, detected a strong band (around 20 kDa) in WB (1:200 dilution).
Date published: 2018-07-09
Rated 5 out of 5 by from SC-31 NRASExcellent antibody. Specifically detects NRAS better than any other tested antibody. Can also be used in IF for overexpression systems (but not endogenous, although no RAS antibodies can be, this was the only one we tested that even worked specifically for overexpression).
Date published: 2017-10-11
Rated 5 out of 5 by from Great Western blot dataThe N-Ras Antibody (F155) shows strong Western blot signal with minimal background!
Date published: 2016-12-23
Rated 5 out of 5 by from Robust staining in paraffinRobust staining in paraffin-embedded human colon tissue but Unfortunately the nucleus have obvious coloring.
Date published: 2015-11-11
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_31, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
N-Ras 抗体 (F155) is rated 4.8 out of 5 by 13.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_31, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 119ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。