Date published: 2025-9-7
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MyoD Antibody (5.8A) - Western Blotting - Image 9954
MyoD 抗体 (5. 8A): sc-32758. 人重组 MyoD 融合蛋白的 Western 印迹分析.
MyoD Antibody (5.8A) - Western Blotting - Image 9954
MyoD 抗体 (5. 8A): sc-32758. 人重组 MyoD 融合蛋白的 Western 印迹分析.
MyoD Antibody (5.8A) - Western Blotting - Image 11964
用 MyoD 抗体 (5. 8A): sc-32758 免疫沉淀并用 MyoD (C-20): sc-304 检测大鼠骨骼肌组织提取物中 MyoD 表达的 Western 印迹分析.
MyoD Antibody (5.8A) - Western Blotting - Image 374634
MyoD 抗体 (5. 8A): sc-32758. Western 印迹分析 C2C12 全细胞裂解液中 MyoD 的表达.
MyoD 抗体 (5. 8A): sc-32758. 人重组 MyoD 融合蛋白的 Western 印迹分析.

MyoD 抗体 (5.8A): sc-32758

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寫評論提問

说明书
  • MyoD抗体(5.8A)是小鼠单克隆IgG1 κ, 在140篇文献中引用，规格为200 µg/ml
  • 针对mouse起源的重组MyoD，其表位与氨基酸180-189对应
  • 抗-MyoD 抗体 (5.8A) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 MyoD; 不会与 other MyoD family members产生交联反应
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-32758 X, 200 µg/0.1 ml)
  • MyoD抗体（5.8A）可与Alexa Fluor® 546或Alexa Fluor® 594结合，用于WB（RGB）、IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MyoD (5.8A): sc-32758的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 MyoD 抗体 (5.8A) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MyoD 抗体 (5.8A) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

MyoD抗体（5.8A）是一种小鼠单克隆IgG1 kappa轻链抗体，专门用于识别肌源分化1（MyoD），它是肌源调控因子（MRFs）家族中的一种关键转录因子，该家族还包括肌源素、Myf5和MRF4（赫库林）。MyoD 单克隆抗体（5.8A）是针对重组小鼠 MyoD 产生的，靶向位于氨基酸 180-189 之间的表位，确保与 MyoD 蛋白精确结合。MyoD通过与DNA中的E-box序列（CANNTG）结合，从而调节对肌肉发育和再生至关重要的基因的表达，在肌肉细胞分化中发挥着重要作用。通过与E2A、IF2和HEB等E蛋白形成异源二聚体，MyoD激活肌肉特异性基因的转录，使MyoD成为肌形成的关键调节因子。MyoD 单克隆抗体（5.8A）推荐用于多种物种（包括小鼠、大鼠和人类）的蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）应用，不会与 MyoD 家族的其他成员发生交叉反应。抗肌分化蛋白抗体（5.8A）有结合和非结合两种形式，是研究肌肉分化与再生分子机制的科学家不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MyoD 抗体 (5.8A) 参考文献:

  1. FHL3 与 MyoD 结合，对肌管的形成起负向调节作用。  |  Cottle, DL., et al. 2007. J Cell Sci. 120: 1423-35. PMID: 17389685
  2. Herculin是MyoD肌生成调控基因家族的第四个成员。  |  Miner, JH. and Wold, B. 1990. Proc Natl Acad Sci U S A. 87: 1089-93. PMID: 2300571
  3. Myf-6，人类肌原决定因子基因家族的新成员：12 号染色体上基因簇的证据。  |  Braun, T., et al. 1990. EMBO J. 9: 821-31. PMID: 2311584
  4. 肌生成素是一种调节肌生成的因子，其结构域与 MyoD 同源。  |  Wright, WE., et al. 1989. Cell. 56: 607-17. PMID: 2537150
  5. 鉴定 MRF4：肌肉调节因子基因家族的新成员。  |  Rhodes, SJ. and Konieczny, SF. 1989. Genes Dev. 3: 2050-61. PMID: 2560751
  6. 一种与 MyoD1 相关但不同于 MyoD1 的新型人类肌肉因子可诱导 10T1/2 成纤维细胞的成肌转换。  |  Braun, T., et al. 1989. EMBO J. 8: 701-9. PMID: 2721498
  7. 寻找MyoD和一路走来的经验教训。  |  Lassar, AB. 2017. Semin Cell Dev Biol. 72: 3-9. PMID: 29097153
  8. 主控：哺乳动物Myod的转录调控。  |  Wardle, FC. 2019. J Muscle Res Cell Motil. 40: 211-226. PMID: 31301002
  9. MyoD诱导的跨分化：剖析细胞承诺、分化与重编程分子机制的典范。  |  Battistelli, C., et al. 2022. Cells. 11: PMID: 36359831
  10. HLH 强迫二聚体：将 MyoD 与 E47 绑定可产生不受 Id 负向调节的显性正性肌生成因子。  |  Neuhold, LA. and Wold, B. 1993. Cell. 74: 1033-42. PMID: 7691411
  11. 一种细胞因子可刺激 MyoD 和 E47 的 DNA 结合活性。  |  Thayer, MJ. and Weintraub, H. 1993. Proc Natl Acad Sci U S A. 90: 6483-7. PMID: 8393567
  12. 利用条件性 MyoD 转录因子研究 MyoD 的反式激活和肌肉决定。  |  Hollenberg, SM., et al. 1993. Proc Natl Acad Sci U S A. 90: 8028-32. PMID: 8396258

订购信息

产品名称产品编号规格价格数量收藏夹

MyoD 抗体 (5.8A)

sc-32758
200 µg/ml
RMB2377.00

MyoD (5.8A): m-IgG Fc BP-HRP 套装

sc-526686
200 µg Ab; 10 µg BP
RMB2662.00

MyoD (5.8A): m-IgG1 BP-HRP 套装

sc-532059
200 µg Ab; 20 µg BP
RMB2662.00

MyoD 抗体 (5.8A) X

sc-32758 X
200 µg/0.1 ml
RMB2377.00

MyoD 抗体 (5.8A) Alexa Fluor® 546

sc-32758 AF546
200 µg/ml
RMB2685.00

MyoD 抗体 (5.8A) Alexa Fluor® 594

sc-32758 AF594
200 µg/ml
RMB2685.00

MyoD 抗体 (5.8A) Alexa Fluor® 680

sc-32758 AF680
200 µg/ml
RMB2685.00

MyoD 抗体 (5.8A) Alexa Fluor® 790

sc-32758 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2017-01-06
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Rated 1 out of 5 by from no signali bought it several months ago after i had seen somebody said signal was good. However, there is really no positive signal when i stained the TA tissue sections. As a positive control, sc-304 myod antibody stained specific and beautiful signal. Why does not produce sc-304?
Date published: 2019-09-25
Rated 4 out of 5 by from Buona reattivitàbuona reattività su rabdomiosarcoma canino a diluizione 1:100 dopo smascheramento con calore in tampone citrato a pH6
Date published: 2018-12-17
Rated 5 out of 5 by from WB on Danio rerio and Natrix natrixI tested antibody on Danio rerio and Natrix natrix embryos. Bands corresponding with molecular weight of MyoD were observed.
Date published: 2018-01-26
Rated 1 out of 5 by from IHC IF on mdx tissueI was able to attain a free sample of this antibody and tried a few different fixatives (2% PFA, 4%PFA and Acetone) including the one that was referenced in the paper. There is no convincing nuclear signal that overlaps with DAPI, which is what is expected for the MyoD transcription factor. No need to include images, as there is no signal.
Date published: 2017-09-03
Rated 5 out of 5 by from Dear professorsI feel good after bought it .And the result is very clear.I like it very much.
Date published: 2017-07-04
Rated 5 out of 5 by from good signalIt gave good tesult, clear band appears at 45 kd by WB
Date published: 2017-05-10
Rated 5 out of 5 by from ProductImmunohistochemical analysis using 1:100 dilution, the staining color is very good, It' useful.
Date published: 2017-04-07
Rated 4 out of 5 by from Ok productFor both wb and ihc not as good as g-1 alternative
Date published: 2016-12-30
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MyoD 抗体 (5.8A) is rated 3.9 out of 5 by 15.
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