Date published: 2025-9-7
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MYL9/MYL12A/B Antibody (E-4) - Western Blotting - Image 354901
MYL9/MYL12A/B 抗体 (E-4): sc-28329. COLO 205 (A) 和 A-431 (B) 全细胞裂解液中 MYL9/MYL12A/B 表达的 Western 印迹分析.
MYL9/MYL12A/B 抗体 (E-4): sc-28329. COLO 205 (A) 和 A-431 (B) 全细胞裂解液中 MYL9/MYL12A/B 表达的 Western 印迹分析.

MYL9/MYL12A/B 抗体 (E-4): sc-28329

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说明书
  • MYL9/MYL12A/B 抗体 E-4 是小鼠单克隆 IgG1 κ，MYL9/MYL12A/B抗体, 在27篇文献中引用，规格为200 µg/ml
  • 针对代表human起源的全长MRCL3的氨基酸1-172
  • MYL9/MYL12A/B 抗体 (E-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测 the myosin regulatory light chains encoded by MYL9, MYL12A and MYL12B, and LOC391722 of human origin; Mylc2b, Myl9 and Myl12a of mouse origin; and Mrlcb and Myl9 of rat origin
  • 抗MYL9/MYL12A/B抗体（E-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MYL9/MYL12A/B (E-4): sc-28329的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 MYL9/MYL12A/B 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MYL9/MYL12A/B 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Myosin 抗体 包括 MRLC2, MYH, MYH1, MYH10, MYH11, MYH15, MYH2, MYH3, MYH4和MYH6

MYL9/MYL12A/B抗体（E-4）是一种鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法，在小鼠、大鼠和人类样本中检测肌球蛋白轻链蛋白，特别是MYL9、MYL12A和MYL12B。MYL9/MYL12A/B (E-4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。肌球蛋白轻链通过与肌动蛋白丝相互作用，在肌肉收缩和细胞运动中发挥着关键作用，而磷酸化状态对于调节这些过程至关重要。肌球蛋白调节轻链的磷酸化，特别是在钙和钙调蛋白存在的情况下，通过肌球蛋白轻链激酶的作用，可增强肌动蛋白激活的肌球蛋白ATP酶活性，这对于肌肉收缩和各种细胞运动至关重要。此外，肌球蛋白轻链在横纹肌中的存在表明其在肌肉功能中可能发挥的作用仍有待充分阐明。了解肌球蛋白轻链磷酸化动力学及其在肌肉生理学和病理学中的意义，有助于深入了解各种肌肉疾病和潜在的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MYL9/MYL12A/B 抗体 (E-4) 参考文献:

  1. 抑制 Rho- 缔合激酶可阻断激动剂诱导的 Ca2+ 对豚鼠回肠肌球蛋白磷酸化和肌力的敏感性。  |  Swärd, K., et al. 2000. J Physiol. 522 Pt 1: 33-49. PMID: 10618150
  2. 平滑肌肌球蛋白轻链激酶 C 端结构域对平滑肌肌球蛋白磷酸化的功能作用  |  Numata, T., et al. 2001. J Biochem. 129: 437-44. PMID: 11226884
  3. Rho 激酶介导血清诱导的成纤维细胞纤维收缩，与肌球蛋白 LC20 磷酸化无关。  |  Nobe, H., et al. 2003. Am J Physiol Cell Physiol. 284: C599-606. PMID: 12388108
  4. ERK 介导的子宫动脉收缩：粗丝和细丝调节途径的作用。  |  Xiao, D., et al. 2004. Am J Physiol Heart Circ Physiol. 286: H1615-22. PMID: 15072969
  5. 导致家族性肥厚型心肌病的肌球蛋白 RLC 的 E22K 突变会增加转基因小鼠对钙的敏感性和 ATP 酶。  |  Szczesna-Cordary, D., et al. 2005. J Cell Sci. 118: 3675-83. PMID: 16076902
  6. 与整合素相连的激酶负责血管平滑肌不依赖于 Ca2+ 的肌球蛋白二磷酸化和收缩。  |  Wilson, DP., et al. 2005. Biochem J. 392: 641-8. PMID: 16201970
  7. 先天性大鼠气道高反应性的平滑肌肌球蛋白同工酶表达和 LC20 磷酸化  |  Gil, FR., et al. 2006. Am J Physiol Lung Cell Mol Physiol. 291: L932-40. PMID: 16766577
  8. 人血管平滑肌肌球蛋白轻链 2 同工酶在非肌肉细胞中的特征和差异表达。  |  Kumar, CC., et al. 1989. Biochemistry. 28: 4027-35. PMID: 2526655
  9. PRPF19通过MYL9的K63连接泛素化激活Src-YAP1通路，从而促进结直肠癌肝转移。  |  Zhou, R., et al. 2023. Cell Death Dis. 14: 258. PMID: 37031206
  10. MYL9在癌相关成纤维细胞中表达，可调节结直肠癌的免疫微环境，并以自泌方式促进肿瘤进展。  |  Deng, S., et al. 2023. J Exp Clin Cancer Res. 42: 294. PMID: 37926835
  11. 鲁帕他定抑制的OTUD3通过去泛素化MYL12A和PD-L1促进DLBCL进展和免疫逃逸。  |  Sui, Y., et al. 2024. Cell Death Dis. 15: 561. PMID: 39097608
  12. 鸡胚成纤维细胞中不依赖 Ca2+ 的肌球蛋白 II 磷酸化和收缩。  |  Kolodney, MS., et al. 1999. J Physiol. 515 (Pt 1): 87-92. PMID: 9925880

订购信息

产品名称产品编号规格价格数量收藏夹

MYL9/MYL12A/B 抗体 (E-4)

sc-28329
200 µg/ml
RMB2377.00

MYL9/MYL12A/B (E-4): m-IgG Fc BP-HRP 套装

sc-528426
200 µg Ab; 10 µg BP
RMB2662.00

MYL9/MYL12A/B (E-4): m-IgGκ BP-HRP 套装

sc-520800
200 µg Ab, 40 µg BP
RMB2662.00

MYL9/MYL12A/B (E-4): m-IgG1 BP-HRP 套装

sc-542927
200 µg Ab; 20 µg BP
RMB2662.00

MYL9/MYL12A/B 抗体 (E-4) AC

sc-28329 AC
500 µg/ml, 25% agarose
RMB3129.00

MYL9/MYL12A/B 抗体 (E-4) HRP

sc-28329 HRP
200 µg/ml
RMB2377.00

MYL9/MYL12A/B 抗体 (E-4) FITC

sc-28329 FITC
200 µg/ml
RMB2482.00

MYL9/MYL12A/B 抗体 (E-4) PE

sc-28329 PE
200 µg/ml
RMB2580.00

MYL9/MYL12A/B 抗体 (E-4) Alexa Fluor® 488

sc-28329 AF488
200 µg/ml
RMB2685.00

MYL9/MYL12A/B 抗体 (E-4) Alexa Fluor® 546

sc-28329 AF546
200 µg/ml
RMB2685.00

MYL9/MYL12A/B 抗体 (E-4) Alexa Fluor® 594

sc-28329 AF594
200 µg/ml
RMB2685.00

MYL9/MYL12A/B 抗体 (E-4) Alexa Fluor® 647

sc-28329 AF647
200 µg/ml
RMB2685.00

MYL9/MYL12A/B 抗体 (E-4) Alexa Fluor® 680

sc-28329 AF680
200 µg/ml
RMB2685.00

MYL9/MYL12A/B 抗体 (E-4) Alexa Fluor® 790

sc-28329 AF790
200 µg/ml
RMB2685.00

I am looking for an AB that will stain phosphorylated LC-20. Can this AB be used for this purpose or do you provide another version of AB that stains for phospho-LC-20 as well?

Asked by: doksana
Thank you for your question. MYL9/MYL12A/B Antibody (E-4): sc-28329 will recognize its target protein in both phosphorylated and unphosphorylated form. The antibody is unable to distinguish between the two forms however.
Answered by: Tech Support Europe
Date published: 2020-07-29

Could I use this MYL9/MYL12A/B Antibody (E-4): sc-28329 antibody for immunofluorescence of human bone marrow-derived mesenchymal stem cells(hBMSCs)? Is it only for smooth muscle myosins, or also for non-muscle myosins? Thanks.

Asked by: taiyang
Thank you for your question. MYL9/MYL12A/B Antibody (E-4): sc-28329 has been raised against amino acids 1-172 representing full length MRCL3 of human origin. It is recommended for recommended for detection of the myosin regulatory light chains encoded by MYL9, MYL12A and MYL12B, and LOC391722 of human origin; Mylc2b, Myl9 and Myl12a of mouse origin; and Mrlcb and Myl9 of rat origin by immunofluorescence, WB, IP, IHC(P) and ELISA. For further information, please contact our technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can I use MYL9/MYL12A/B (E-4): sc-28329 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: cjMara
Yes. We recommend either using a directly conjugated monoclonal antibody. MYL9/MYL12A/B (E-4): sc-28329 monoclonal antibody is available directly conjugated to either HRP, FITC, PE, Alexa Fluor® 488 or Alexa Fluor® 647. Alternatively, we recommend performing indirect staining using our recommended mouse IgG binding protein conjugated to HRP (sc-516102). https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp?requestFrom=search
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 1 out of 5 by from does not work for WBused this antibody 1:100 the same day it arrived for western blot. it did not work. With the same membrane I blotted for phospho-MLC2 (Rabbit antibody0, and it works beautifully.
Date published: 2023-04-04
Rated 1 out of 5 by from The Myl9(E4) didn't workThe Myl9(E4) didn't work in C2C12, HEK293, MDCK and Rat2 cell lysates. I used 1:1000 dilution.
Date published: 2017-12-04
Rated 4 out of 5 by from Buen resultadoNos mandaron la prueba del anticuerpo hace dos semanas. Lo usamos en western blot, en muestras invitro, con una concentración de 1:1000 y con 1% de BSA en TBST. Funcionó bastante bien, se veía una señal clara y no necesitaba mucha exposición. Compraremos el original próximamente.
Date published: 2017-04-19
Rated 5 out of 5 by from Just a great productOutstanding product for western blot, IF and IHC staining
Date published: 2017-02-05
Rated 5 out of 5 by from Clean WB Results!Great performance in Western, clean bands with no background. Also good in IF and IHC.
Date published: 2016-12-23
Rated 5 out of 5 by from Good antibodyThis MRLC antibody is good for Western blot experiment in C2C12 cells.
Date published: 2016-11-02
Rated 5 out of 5 by from Western blot publication using MCFWestern blot publication using MCF-10A samples -SCBT Publication Review
Date published: 2015-03-27
Rated 5 out of 5 by from Mouse astrocytes published in WB applicationMouse astrocytes published in WB application -SCBT Publication Review
Date published: 2015-03-23
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MYL9/MYL12A/B抗体 (E-4) is rated 4.2 out of 5 by 11.
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