Date published: 2025-9-8
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MYH Antibody (B-5) - Western Blotting - Image 286126
MYH 抗体 (B-5): sc-376157. 人骨骼肌 (A) 和大鼠骨骼肌 (B) 组织提取物中 MYH 表达的 Western 印迹分析.
MYH 抗体 (B-5): sc-376157. 人骨骼肌 (A) 和大鼠骨骼肌 (B) 组织提取物中 MYH 表达的 Western 印迹分析.

MYH 抗体 (B-5): sc-376157

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说明书
  • MYH 抗体 B-5 是小鼠单克隆 IgG1 κ，MYH抗体, 在106篇文献中引用，规格为200 µg/ml
  • 免疫human物种的myosin heavy chain 3的氨基酸1641-1940
  • MYH 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 skeletal and cardiac myosin heavy chain
  • 抗MYH抗体（B-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MYH (B-5): sc-376157的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 MYH 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MYH 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Myosin抗体，包括MRLC2, MYH, MYH1, MYH10, MYH11, MYH15, MYH2, MYH3, MYH4, MYH6, MYH7, MYH9, MYL, MYL1, MYL2和MYL3

MYH抗体（B-5）是一种IgG1 κ小鼠单克隆MYH抗体（也称为肌肉胚胎肌球蛋白抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的MYH蛋白。MYH抗体（B-5）既可作为非偶联抗MYH抗体形式提供，也可作为多种偶联形式的抗MYH抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肌球蛋白是一种高度保守、普遍表达的蛋白质，与肌动蛋白相互作用以产生细胞运动所需的力。常规肌球蛋白是由两个重链亚基、一对非磷酸化轻链亚基和一对磷酸化轻链亚基组成的六聚体蛋白质。已经克隆出三类肌球蛋白：平滑肌肌球蛋白、横纹肌肌球蛋白和非肌肉肌球蛋白。平滑肌的收缩活动受肌球蛋白轻链激酶对肌球蛋白轻链（MLC）的钙/钙调蛋白依赖性磷酸化的调节。由MYH基因家族编码的肌球蛋白重链具有肌动蛋白激活的ATP酶活性，可产生肌球蛋白的运动功能。肌球蛋白重链最初是从人胎儿骨骼肌中分离出来的，是决定骨骼肌收缩速度的主要因素。不同功能的肌肉中，会差异性表达各种肌球蛋白重链异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MYH 抗体 (B-5) 参考文献:

  1. 骨骼肌发育过程中肌球蛋白重链基因表达的时空变化  |  Lu, BD., et al. 1999. Dev Biol. 216: 312-26. PMID: 10588881
  2. 鉴定人类肌球蛋白重链的三种发育受控异构体  |  Bober, E., et al. 1990. Eur J Biochem. 189: 55-65. PMID: 1691980
  3. 生成全长的人类围产期肌球蛋白重链编码 cDNA。  |  Karsch-Mizrachi, I., et al. 1990. Gene. 89: 289-94. PMID: 2373371
  4. 通过 cDNA 克隆和免疫印迹分析鉴定两种平滑肌肌球蛋白重链同工酶。  |  Nagai, R., et al. 1989. J Biol Chem. 264: 9734-7. PMID: 2722872
  5. 优秀手球运动员三角肌和股外侧肌肌球蛋白重链同工型组成。  |  Mandroukas, A., et al. 2020. J Sports Sci. 38: 2390-2395. PMID: 32602402
  6. 人类围产期肌球蛋白重链转录本的特征。  |  Jullian, EH., et al. 1995. Eur J Biochem. 230: 1001-6. PMID: 7601129
  7. 调节血管平滑肌细胞的分化。  |  Owens, GK. 1995. Physiol Rev. 75: 487-517. PMID: 7624392
  8. 肌球蛋白超家族的系统发育分析。  |  Cheney, RE., et al. 1993. Cell Motil Cytoskeleton. 24: 215-23. PMID: 8477454
  9. 平滑肌收缩机制  |  Horowitz, A., et al. 1996. Physiol Rev. 76: 967-1003. PMID: 8874491
  10. 哺乳动物肌球蛋白重链基因家族。  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733
  11. 平滑肌肌球蛋白重链同工型。  |  Loukianov, E., et al. 1997. Comp Biochem Physiol B Biochem Mol Biol. 117: 13-8. PMID: 9180010
  12. 发育过程中的肌球蛋白重链转换。对呼吸肌的功能影响。  |  Watchko, JF., et al. 1998. Comp Biochem Physiol B Biochem Mol Biol. 119: 459-70. PMID: 9734330

订购信息

产品名称产品编号规格价格数量收藏夹

MYH 抗体 (B-5)

sc-376157
200 µg/ml
RMB2377.00

MYH (B-5): m-IgGκ BP-HRP 套装

sc-522854
200 µg Ab, 40 µg BP
RMB2662.00

MYH (B-5): m-IgG1 BP-HRP 套装

sc-533527
200 µg Ab; 20 µg BP
RMB2662.00

MYH 抗体 (B-5) AC

sc-376157 AC
500 µg/ml, 25% agarose
RMB3129.00

MYH 抗体 (B-5) HRP

sc-376157 HRP
200 µg/ml
RMB2377.00

MYH 抗体 (B-5) FITC

sc-376157 FITC
200 µg/ml
RMB2482.00

MYH 抗体 (B-5) PE

sc-376157 PE
200 µg/ml
RMB2580.00

MYH 抗体 (B-5) Alexa Fluor® 488

sc-376157 AF488
200 µg/ml
RMB2685.00

MYH 抗体 (B-5) Alexa Fluor® 546

sc-376157 AF546
200 µg/ml
RMB2685.00

MYH 抗体 (B-5) Alexa Fluor® 594

sc-376157 AF594
200 µg/ml
RMB2685.00

MYH 抗体 (B-5) Alexa Fluor® 647

sc-376157 AF647
200 µg/ml
RMB2685.00

MYH 抗体 (B-5) Alexa Fluor® 680

sc-376157 AF680
200 µg/ml
RMB2685.00

MYH 抗体 (B-5) Alexa Fluor® 790

sc-376157 AF790
200 µg/ml
RMB2685.00

Hi, We have already purchased the MYH skeletal muscle last years in August. It stopped working and now we want to try this antibody. Any possibility send us the sample for testing before buying in bulk. Thank you Shabir

Asked by: SHABIR ZATHAT
Thank you for your question. Please contact technical service for assistance. Please email [email protected] or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2025-03-27

To what part of MUTYH (MYH) does the antibody bind to? N-terminus, C-Terminus, or to the middle of the protein?

Asked by: cavasqu1
Thank you for your question. MYH Antibody (B-5): sc-376157 was raised against amino acids 1641-1940 of myosin heavy chain 3 of human origin, which corresponds to C-terminus of the protein.
Answered by: Tech Support Europe
Date published: 2021-07-15

Do mouse MYH2, MYH6, MYH7 can be detected by MYH(B-5) in WB?

Asked by: SYH05
Thank you for your question. This MYH antibody, sc-376157, will for sure detect MYH3, but has not been epitope mapped so we can not guarantee that it can pick up MYH2, MYH7 or MYH6. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-10-19

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-24
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Rated 1 out of 5 by from Does not stain muscle fibersWe are trying to do IF of MyHC in C2C12 myotubes...I am not geting good results.
Date published: 2025-07-28
Rated 4 out of 5 by from GoodMeets what was promised for WB, recognizes MYH in rat ventricular extract
Date published: 2019-09-03
Rated 5 out of 5 by from Great Technical Service and Great ProductI purchased this product one month ago and I must admit, I did have some bumps in the road when it came to receipt of the product and full understanding of the different variations. However, those bumps were easily smoothed out with a quick call to customer service and technical service helped me understand the product entirely. I'm very happy I ordered my antibodies from Santa Cruz Bio Technology.
Date published: 2017-07-07
Rated 2 out of 5 by from Weak for immunofluorescenceI used this antibody in thick sections (200 um) of breast xenografts. The staining is not very specific and weak.
Date published: 2017-06-26
Rated 5 out of 5 by from Very good in WB in C2C12 cellsCompare to MYH7 (A4.840), MYH （B5）shows strong signal in C2C12 differentiation for 7 days. It is a good antibody for WB in C2C12.
Date published: 2017-05-31
Rated 5 out of 5 by from Nice for WBThe MYH(B-5) mouse monoclonal antibody is very nice for Western blotting!
Date published: 2017-05-24
Rated 5 out of 5 by from Very good antibody for WBVery good antibody for WB and excellent immunohistochemistry, I suggest dilution this antibody at 1:200.
Date published: 2017-02-09
Rated 5 out of 5 by from superior replacementMYH Antibody (B-5) is raised against the same recombinant protein immunogen used to develop the rabbit polyclonal sc-20641 (H-300) antibody. This new monoclonal antibody is a superior replacement with greater specificity and no lot-to-lot variation.
Date published: 2017-01-07
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MYH 抗体 (B-5) is rated 4.1 out of 5 by 9.
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